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Abstract

A Gram-stain-negative, aerobic, non-motile, long rods with no flagellum strain, designated THG-YS3.2.1, was isolated from rhizosphere soil of mugunghwa, collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10−40 °C (optimum 28 °C), at pH 6.0−8.0 (optimum pH 7.0) and with 0−1.0 % NaCl (optimum 1.0 %). The predominant menaquinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso-C, iso-C, iso-C, anteiso-C, iso-C G, anteiso-C A, C 2-OH, C, iso-C, C 3-OH, iso-C 3-OH, iso-C G, C 2-OH, iso-C 3-OH, Cω6, Cω6 (6, 9, 12), C, summed feature 3 (Cω7 and/or iso-C 2-OH). The major polar lipids were phosphatidylmethylethanolamine), phosphatidylethanolamine, five unidentified aminolipids and three unidentified lipids. The DNA G+C content of strain THG-YS3.2.1 was 45.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.2.1 were KCTC 22560 (98.7 % 16S rRNA gene sequence similarity), KACC 17399 (96.9 %), KCTC 42474 (96.2 %), KACC 11466 (95.5 %) and KACC 11465 (95.1 %). DNA–DNA hybridization values between strain THG-YS3.2.1 and KCTC 22560, KACC 17399, . KCTC 42474, KACC 11466 and KACC 11465were 55.8±1.0, 39.5±0.5, 35.2±0.1, 17.6±0.3 and 12.5±1.2 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA−DNA hybridization data, strain THG-YS3.2.1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is THG-YS3.2.1 (=KCTC 52084=CCTCC AB 2015356).

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2016-12-01
2022-01-21
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