1887

Abstract

A novel actinomycete, strain TRM 41495, was isolated from lake sediment of Xiaoerkule lake in Xinjiang province, China, and was examined using a polyphasic approach. Strain TRM 41495 was aerobic, Gram-stain-positive, growing best on ISP medium 7 with abundant white aerial mycelium formed and brown soluble pigments produced. The optimum NaCl concentration for growth was 3.0 % (w/v), and the optimum temperature for growth was 37 °C. On the basis of 16S rRNA gene sequence analysis, strain TRM 41495 was shown to belong to the genus with a sequence similarity of 97.50 % with the most closely related species, . The organism had chemical and morphological features typical of the genus . The cell wall of strain TRM 41495 contained -diaminopimelic acid, and galactose, arabinose, ribose and mannose were the major whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and an unknown phosphoglycolipid. The predominant menaquinones were MK-9(H) and MK-9(H). The major fatty acids were iso-C, C, Cω6, anteiso-C and iso-CH. The G+C content of the DNA was 72.9 mol%. However, DNA–DNA hybridization studies between strain TRM 41495 and showed only 43.9 % relatedness. Whole-cell sugars, menaquinones and fatty acids differentiate strain TRM 41495 from . On the basis of the polyphasic evidence, strain TRM 41495 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM 41495 (=CCTCC AA 2015038=KCTC 39727).

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2016-12-01
2021-07-30
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