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Abstract

A Gram-stain-negative, light pink, non-motile, rod-shaped, aerobic bacterium, designated strain XNV015, was isolated from soil of a vanadium mine. Phylogenetic analyses based on 16S rRNA gene sequences indicated that it belongs to the genus Pedobacter and was closely related to Pedobacter suwonensis DSM 18130 (96.93 % sequence similarity), Pedobacter alluvionis NWER-II11 (96.66 %), Pedobacter terrae DS-57 (96.54 %), Pedobacter kyungheensis KACC 16221 (96.54 %) and Pedobacter soli KACC 14939 (96.47 %). This strain clearly differed from the closely related species in terms of acid production from rhamnose and ethanol. Menaquinone-7 was the predominant respiratory quinone. The predominant fatty acids included iso-C15 : 0, C16 : 1 ω5c, summed feature 3, iso-C17 : 0 3-OH and C17 : 0 2-OH. The major polar lipids were phosphatidylethanolamine, glycolipids, lipids and aminolipids. The genomic DNA G+C content was 43.8 mol%. The genotypic analysis, biochemical properties, and phenotypic and chemotaxonomic characteristics indicate that strain XNV015 represents a novel species of the genus Pedobacter , for which the name Pedobacter vanadiisoli sp. nov. is proposed. The type strain is XNV015 (=CCTCC AB 2015319=KCTC 42866).

Keyword(s): Pedobacter and vanadium mine soil
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2016-12-01
2019-10-21
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