1887

Abstract

Strain Eup-16, isolated from the torch coral , was characterized using a polyphasic taxonomy approach. Cells of strain Eup-16 were Gram-staining-negative, aerobic, motile by means of a single polar flagellum, contained poly-β-hydroxybutyrate, rod-shaped and formed pale yellow colonies. Optimal growth occurred at 25–30 °C, pH 7.5–9 and in the presence of 1 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Eup-16 belonged to the genus and was most closely related to CL-22 with sequence similarity of 98.4 %. Strain Eup-16 contained summed feature 3 (Cω7 and/or Cω6), Cω7, C and Cω8 as the predominant fatty acids. The predominant isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content of strain Eup-16 was 43.2 mol%. The DNA–DNA hybridization value for strain Eup-16 with CL-22 was less than 34 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain Eup-16 should be classified as a representative of a novel species of the genus , for which the name sp. nov. is presented. The type strain is Eup-16 (=BCRC 80910=LMG 29001=KCTC 42743).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001466
2016-12-01
2021-09-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5039.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001466&mimeType=html&fmt=ahah

References

  1. Anzai Y., Kudo Y., Oyaizu H. 1997; The phylogeny of the genera Chryseomonas, Flavimonas, and Pseudomonas supports synonymy of these three genera. Int J Syst Bacteriol 47:249–251 [View Article][PubMed]
    [Google Scholar]
  2. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  3. Bowman J. P., McMeekin T. A. 2005; Order X. Alteromonadales ord. nov. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2 pp. 443–490 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer; [CrossRef]
    [Google Scholar]
  4. Chen W. M., Laevens S., Lee T. M., Coenye T., De Vos P., Mergeay M., Vandamme P. 2001; Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51:1729–1735 [View Article][PubMed]
    [Google Scholar]
  5. Chen W. M., Liu L. P., Chen C. A., Wang J. T., Sheu S. Y. 2016; Thalassotalea montiporae sp. nov., isolated from the encrusting pore coral Montipora aequituberculata . Int J Syst Evol Microbiol 66:4077–4084 [CrossRef]
    [Google Scholar]
  6. Cole J. R., Wang Q., Cardenas E., Fish J., Chai B., Farris R. J., Kulam-Syed-Mohideen A. S., McGarrell D. M., Marsh T. et al. 2009; The Ribosomal database project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res 37:D141–D145 [View Article][PubMed]
    [Google Scholar]
  7. Collins M. D. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp. 265–309 Edited by Goodfellow M., O’Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  8. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. Chemical Methods in Prokaryotic Systematics pp. 121–161 Edited by Goodfellow M., O’Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  9. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  10. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  11. Felsenstein J. 1993; PHYLIP (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA
  12. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  13. Hosoya S., Adachi K., Kasai H. 2009; Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. Int J Syst Evol Microbiol 59:686–690 [View Article][PubMed]
    [Google Scholar]
  14. Hou T. T., Liu Y., Zhong Z. P., Liu H. C., Liu Z. P. 2015; Thalassotalea marina sp. nov., isolated from a marine recirculating aquaculture system, reclassification of Thalassomonas eurytherma as Thalassotalea eurytherma comb. nov. and emended description of the genus Thalassotalea . Int J Syst Evol Microbiol 65:4710–4715 [View Article][PubMed]
    [Google Scholar]
  15. Jung Y.-T., Park S., Yoon J.-H. 2014; Thalassomonas fusca sp. nov., a novel gammaproteobacterium isolated from tidal flat sediment. Antonie van Leeuwenhoek 105:81–87 [View Article][PubMed]
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  17. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  18. Kluge A. G., Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  19. Kumar S., Stecher G., Tamura K. 2016; mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  21. Nokhal T-H., Schlegel H. G. 1983; Taxonomic study of Paracoccus denitrificans . Int J Syst Bacteriol 33:26–37 [View Article]
    [Google Scholar]
  22. Park S., Jung Y.-T., Kang C.-H., Park J.-M., Yoon J.-H. 2014; Thalassotalea ponticola sp. nov., isolated from seawater, reclassification of Thalassomonas fusca as Thalassotalea fusca comb. nov. and emended description of the genus Thalassotalea . Int J Syst Evol Microbiol 64:3676–3682 [View Article][PubMed]
    [Google Scholar]
  23. Powers E. M. 1995; Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61:3756–3758[PubMed]
    [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  25. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  26. Schlegel H. G., Lafferty R., Krauss I. 1970; The isolation of mutants not accumulating poly-beta-hydroxybutyric acid. Arch Mikrobiol 71:283–294 [View Article][PubMed]
    [Google Scholar]
  27. Spiekermann P., Rehm B. H., Kalscheuer R., Baumeister D., Steinbüchel A. 1999; A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol 171:73–80 [View Article][PubMed]
    [Google Scholar]
  28. Thompson F. L., Barash Y., Sawabe T., Sharon G., Swings J., Rosenberg E. 2006; Thalassomonas loyana sp. nov., a causative agent of the white plague-like disease of corals on the Eilat coral reef. Int J Syst Evol Microbiol 56:365–368 [View Article][PubMed]
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  30. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematic. In Methods for General and Molecular Bacteriology, 3rd edn. pp. 330–393 Edited by Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R. 1987; International committee on systematic bacteriology. report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  32. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703 [View Article][PubMed]
    [Google Scholar]
  33. Wen C. M., Tseng C. S., Cheng C. Y., Li Y. K. 2002; Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2. Biotechnol Appl Biochem 35:213–219 [View Article][PubMed]
    [Google Scholar]
  34. Yi H., Bae K. S., Chun J. 2004; Thalassomonas ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:377–380 [View Article][PubMed]
    [Google Scholar]
  35. Zhang Y., Tang K., Shi X., Zhang X.-H. 2014; Description of Thalassotalea piscium gen. nov., sp. nov., isolated from flounder (Paralichthys olivaceus), reclassification of four species of the genus Thalassomonas as members of the genus Thalassotalea gen. nov. and emended description of the genus Thalassomonas . Int J Syst Evol Microbiol 64:1223–1228 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001466
Loading
/content/journal/ijsem/10.1099/ijsem.0.001466
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error