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Abstract

A taxonomic study using a polyphasic approach was carried out on strain PB8B, which was isolated from the deep water of the Indian Ocean. Cells of the bacterium were Gram-stain-negative, oxidase- and catalase-positive, curved rods and motile. Growth was observed at salinities of 0–15 % and at temperatures of 10–41°C. The isolate could reduce nitrate to nitrite and degrade Tween 80, but not degrade gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PB8B belonged to the genus , with the highest sequence similarity to the closely related type strain 1-1B (99.7 %), followed by WP0211 (99.6 %). Multilocus sequence analysis demonstrated low similarities of 94.1 and 93.7 % between strain PB8B and the two reference type strains. Digital DNA–DNA hybridization values between strain PB8B and the two above-mentioned type strains were, respectively, 56.3 and 55.3 %. The principal fatty acids of strain PB8B were C 6/C 7, C cyclo 8 and C. The G+C content of the chromosomal DNA was 54.9 mol%. The quinone was determined to be Q-10 (100 %). Phosphatidylglycerol, phosphatidylethanolamine, and several unidentified phospholipids and lipids were present. Based on phenotypic and genotypic characteristics, strain PB8B represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain of the novel species is PB8B (=MCCC 1A01103=LMG 29620).

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2016-12-01
2024-12-06
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References

  1. Auch A. F., Klenk H. P., Göker M. 2010a; Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2:142–148 [View Article][PubMed]
    [Google Scholar]
  2. Auch A. F., von Jan M., Klenk H. P., Göker M. 2010b; Digital DNA–DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2:117–134 [View Article][PubMed]
    [Google Scholar]
  3. Cao J., Lai Q., Liu Y., Li G., Shao Z. 2014; Ottowia beijingensis sp. nov., isolated from coking wastewater activated sludge, and emended description of the genus Ottowia . Int J Syst Evol Microbiol 64:963–967 [View Article][PubMed]
    [Google Scholar]
  4. Collins M. D. 1985; Isoprenoid quinone analyses in bacterial classification and identification. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series No. 20) pp 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  5. Dong X., Cai M. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press;
    [Google Scholar]
  6. Ivanova E. P., López-Pérez M., Webb H. K., Ng H. J., Dang T. H., Zhukova N. V., Mikhailov V. V., Crawford R. J., Rodriguez-Valera F. 2016; Thalassospira australica sp. nov. isolated from sea water. Antonie van Leeuwenhoek 109:1091–1100 [View Article][PubMed]
    [Google Scholar]
  7. Kates M. 1986 Techniques of Lipidology, 2 rev ed. Netherlands: Elsevier;
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  9. Kodama Y., Stiknowati L. I., Ueki A., Ueki K., Watanabe K. 2008; Thalassospira tepidiphila sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from seawater. Int J Syst Evol Microbiol 58:711–715 [View Article][PubMed]
    [Google Scholar]
  10. Lai Q., Liu Y., Shao Z. 2014a; Bacillus xiamenensis sp. nov., isolated from intestinal tract contents of a flathead mullet (Mugil cephalus) . Antonie van Leeuwenhoek 105:99–107 [View Article][PubMed]
    [Google Scholar]
  11. Lai Q., Liu Y., Yuan J., Du J., Wang L., Sun F., Shao Z. 2014b; Multilocus sequence analysis for assessment of phylogenetic diversity and biogeography in Thalassospira bacteria from diverse marine environments. PLoS One 9:e106353 [View Article][PubMed]
    [Google Scholar]
  12. Liu C., Wu Y., Li L., Ma Y., Shao Z. 2007; Thalassospira xiamenensis sp. nov. and Thalassospira profundimaris sp. nov. Int J Syst Evol Microbiol 57:316–320 [View Article][PubMed]
    [Google Scholar]
  13. Liu Y., Lai Q., Du J., Shao Z. 2016; Bacillus zhangzhouensis sp. nov. and Bacillus australimaris sp. nov. Int J Syst Evol Microbiol 66:1193–1199 [CrossRef]
    [Google Scholar]
  14. López-López A., Pujalte M. J., Benlloch S., Mata-Roig M., Rosselló-Mora R., Garay E., Rodríguez-Valera F. 2002; Thalassospira lucentensis gen. nov., sp. nov., a new marine member of the alpha-Proteobacteria . Int J Syst Evol Microbiol 52:1277–1283 [View Article][PubMed]
    [Google Scholar]
  15. Meier-Kolthoff J. P., Auch A. F., Klenk H. P., Göker M. 2013; Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14:60 [View Article][PubMed]
    [Google Scholar]
  16. Nogi Y., Yoshizumi M., Miyazaki M. 2014; Thalassospira povalilytica sp. nov., a polyvinyl-alcohol-degrading marine bacterium. Int J Syst Evol Microbiol 64:1149–1153 [View Article][PubMed]
    [Google Scholar]
  17. Plotnikova E. G., Anan'ina L. N., Krausova V. I., Ariskina E. V., Prisyazhnaya N. V., Lebedev A. T., Demakov V. A., Evtushenko L. I. 2011; Thalassospira permensis sp. nov., a new terrestrial halotolerant bacterium isolated from a naphthalene-utilizing microbial consortium. Mikrobiologiia 80:691–699 [View Article][PubMed]
    [Google Scholar]
  18. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark. DE: MIDI Inc;
    [Google Scholar]
  19. Shivaji S., Sathyanarayana Reddy G., Sundareswaran V. R., Thomas C. 2015; Description of Thalassospira lohafexi sp. nov., isolated from Southern Ocean, Antarctica. Arch Microbiol 197:627–637 [View Article][PubMed]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  21. Tsubouchi T., Ohta Y., Haga T., Usui K., Shimane Y., Mori K., Tanizaki A., Adachi A., Kobayashi K. et al. 2014; Thalassospira alkalitolerans sp. nov. and Thalassospira mesophila sp. nov., isolated from a decaying bamboo sunken in the marine environment, and emended description of the genus Thalassospira . Int J Syst Evol Microbiol 64:107–115 [View Article][PubMed]
    [Google Scholar]
  22. Wayne L. G., Brenner D., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  23. Zhao B., Wang H., Li R., Mao X. 2010; Thalassospira xianhensis sp. nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium. Int J Syst Evol Microbiol 60:1125–1129 [View Article][PubMed]
    [Google Scholar]
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