1887

Abstract

During a survey of yeasts associated with insects in Central China's natural ecosystems, 116 yeast strains were isolated from the gut of adult insects in two families and from one beetle larva. Among the yeasts isolated in this study, 102 strains were identified as 20 known species in the class . The remaining 14 strains were identified as representing five novel species in the clade based on the combined sequences of the D1/D2 domains of the LSU rRNA gene and the internal transcribed spacer (ITS) regions, as well as other taxonomic characteristics. sp. nov. (type strain CBS 14171=CICC 33087=NYNU 15764T) formed a clade with and . The other four novel species, namely sp. nov. (type strain CBS 14175=CICC 33091=NYNU 15857), sp. nov. (type strain CBS 13923=CICC 33056=NYNU 1488), sp. nov. (type strain CBS 13928=CICC 33058=NYNU 14959) and sp. nov. (type strain CBS 14180=CICC 33084=NYNU 1582), showed close relationships to the species near , , and . Descriptions of these novel yeast species are provided as well as discussions of their ecology in relation to their insect hosts.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001446
2016-11-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/11/4881.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001446&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  2. Chang C. F., Lin Y. C., Chen S. F., Carvajal Barriga E. J., Carvaja Barriga E. J., Barahona P. P., James S. A., Bond C. J., Roberts I. N., Lee C. F. 2012; Candida theae sp. nov., a new anamorphic beverage-associated member of the Lodderomyces clade. Int J Food Microbiol 153:10–14 [View Article][PubMed]
    [Google Scholar]
  3. Daniel H. M., Lachance M. A., Kurtzman C. P. 2014; On the reclassification of species assigned to Candida and other anamorphic ascomycetous yeast genera based on phylogenetic circumscription. Antonie van Leeuwenhoek 106:67–84 [View Article][PubMed]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  5. Gouliamova D. E., Dimitrov R. A., Smith M. T., Groenewald M., Stoilova-Disheva M. M., Guéorguiev B. V., Boekhout T. 2016; DNA barcoding revealed Nematodospora valgi gen. nov., sp. nov. and Candida cetoniae sp. nov. in the Lodderomyces clade. Fungal Biol 120:179–190 [View Article][PubMed]
    [Google Scholar]
  6. Hui F. L., Niu Q. H., Ke T., Li Y. X., Lee C. F. 2013; Candida sanyaensis sp. nov., an ascomycetous yeast species isolated from soil. Antonie van Leeuwenhoek 103:47–52 [View Article][PubMed]
    [Google Scholar]
  7. Ji Z. H., Jia J. H., Bai F. Y. 2009; Four novel Candida species in the Candida albicans/Lodderomyces elongisporus clade isolated from the gut of flower beetles. Antonie van Leeuwenhoek 95:23–32 [View Article][PubMed]
    [Google Scholar]
  8. Jindamorakot S., Limtong S., Yongmanitchai W., Tuntirungkij M., Potacharoen W., Kawasaki H., Nakase T. 2007; Two new anamorphic yeasts, Candida thailandica sp. nov. and Candida lignicola sp. nov., isolated from insect frass in Thailand. FEMS Yeast Res 7:1409–1414 [View Article][PubMed]
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  10. Kurtzman C. P. 2011; Lodderomyces van der Walt (1971). In The Yeasts, a Taxonomic Study, 5th edn. vol. 2 pp. 561–563 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  11. Kurtzman C. P., Fell J. W., Boekhout T., Robert V. 2011; Methods for isolation, phenotypic characterization and maintenance of yeasts. In The Yeasts, a Taxonomic Study, 5th edn. vol. 1 pp. 87–110 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  12. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371[PubMed] [CrossRef]
    [Google Scholar]
  13. Lachance M. A., Boekhout T., Scorzetti G., Fell J. W., Kurtzman C. P. 2011; Candida Berkhout (1923). In The Yeasts, a Taxonomic Study, 5th edn. vol. 2 pp. 987–1278 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  14. Lachance M. A., Ewing C. P., Bowles J. M., Starmer W. T. 2005; Metschnikowia hamakuensis sp. nov., Metschnikowia kamakouana sp. nov. and Metschnikowia mauinuiana sp. nov., three endemic yeasts from Hawaiian nitidulid beetles. Int J Syst Evol Microbiol 55:1369–1377 [View Article][PubMed]
    [Google Scholar]
  15. Limtong S., Koowadjanakul N., Jindamorakot S., Yongmanitchai W., Nakase T. 2012; Candida sirachaensis sp. nov. and Candida sakaeoensis sp. nov. two anamorphic yeast species from phylloplane in Thailand. Antonie van Leeuwenhoek 102:221–229 [View Article]
    [Google Scholar]
  16. Nakase T., Jindamorakot S., Ninomiya S., Imanishi Y., Kawasaki H. 2009; Candida wancherniae sp. nov. and Candida morakotiae sp. nov., two novel ascomycetous anamorphic yeast species found in Thailand. J Gen Appl Microbiol 55:93–100 [View Article][PubMed]
    [Google Scholar]
  17. Nguyen N. H., Suh S. O., Blackwell M. 2007; Five novel Candida species in insect-associated yeast clades isolated from Neuroptera and other insects. Mycologia 99:842–858 [View Article][PubMed]
    [Google Scholar]
  18. Nguyen N. H., Suh S. O., Marshall C. J., Blackwell M. 2006; Morphological and ecological similarities: wood-boring beetles associated with novel xylose-fermenting yeasts, Spathaspora passalidarum gen. sp. nov. and Candida jeffriesii sp. nov. Mycol Res 110:1232–1241 [View Article][PubMed]
    [Google Scholar]
  19. Nitiyon S., Boonmak C., Am-In S., Jindamorakot S., Kawasaki H., Yongmanitchai W., Limtong S. 2011; Candida saraburiensis sp. nov. and Candida prachuapensis sp. nov., xylose-utilizing yeast species isolated in Thailand. Int J Syst Evol Microbiol 61:462–468 [View Article]
    [Google Scholar]
  20. Ren Y. C., Xu L. L., Zhang L., Hui F. L. 2015; Candida baotianmanensis sp. nov. and Candida pseudoviswanathii sp. nov., two ascosporic yeast species isolated from the gut of beetles. Int J Syst Evol Microbiol 65:3580–3585 [View Article][PubMed]
    [Google Scholar]
  21. Sipiczki M. 2013; Detection of yeast species also occurring in substrates associated with animals and identification of a novel dimorphic species in Verbascum flowers from Georgia. Antonie van Leeuwenhoek 103:567–576 [View Article][PubMed]
    [Google Scholar]
  22. Suh S. O., McHugh J. V., Blackwell M. 2004; Expansion of the Candida tanzawaensis yeast clade: 16 novel Candida species from basidiocarp-feeding beetles. Int J Syst Evol Microbiol 54:2409–2429 [View Article][PubMed]
    [Google Scholar]
  23. Suh S. O., Nguyen N. H., Blackwell M. 2006; A yeast clade near Candida kruisii uncovered: nine novel Candida species associated with basidioma-feeding beetles. Mycol Res 110:1379–1394 [View Article][PubMed]
    [Google Scholar]
  24. Suh S. O., Nguyen N. H., Blackwell M. 2008; Yeasts isolated from plant-associated beetles and other insects: seven novel Candida species near Candida albicans. FEMS Yeast Res 8:88–102 [View Article][PubMed]
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  27. Urbina H., Schuster J., Blackwell M. 2013; The gut of Guatemalan passalid beetles: a habitat colonized by cellobiose- and xylose-fermenting yeasts. Fungal Ecology 6:339–355 [View Article]
    [Google Scholar]
  28. White T. J., Bruns T., Lee S., Taylor J. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide for Methods and Applications pp. 315–322 Edited by Innis M. A., Gelfand D. H., Sninsky J. J., Taylor T. J. New York: Academic;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001446
Loading
/content/journal/ijsem/10.1099/ijsem.0.001446
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error