1887

Abstract

A halotolerant actinobacterium, designated J12GA03, was isolated from a rhizosphere soil sample of Suaeda salsa collected from a dried saline lake in Hebei Province, China. Cells were Gram-staining-positive, non-motile and non-spore-forming cocci. Strain J12GA03 grew optimally at 28‒37 °C, 0‒3 % NaCl (w/v) and pH 6.5‒7.5. It contained meso-diaminopimelic acid as the diagnostic diamino acid and arabinose, galactose and ribose as the diagnostic whole-cell sugars. MK-8 and MK-7 were detected as predominant menaquinones. Major fatty acids were C17 : 1 ω8c, C16 : 0 and C17 : 0. Polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphoglycolipids, glycolipids, unidentified phospholipids and additional lipids. The muramyl residue was acetyl. Mycolic acids (34–38 carbon atoms) were present. The G+C content of the genomic DNA was 55.8 mol%. It shared the highest 16S rRNA gene sequence similarities with Amycolicicoccus subflavus DQS3-9A1 (98.18 %) and Hoyosella altamirensis OFN S31 (97.75 %). Phylogenetic trees showed that strain J12GA03 firmly formed a distinct monophyletic branch in the clade with A . subflavus DQS3-9A1 and H . altamirensis DSM 45258. The levels of DNA–DNA relatedness with A . subflavus DSM 45089 and H . altamirensis DSM 45258 were 39.7±3.9 % and 35.7±3.0 %, respectively. Combining the evidence from the polyphasic taxonomic study, strain J12GA03 represents a novel species of the genus Hoyosella , for which the name Hoyosella rhizosphaerae sp. nov. is proposed. The type strain is J12GA03 (=DSM 101985=CGMCC 1.15478).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001416
2016-11-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/11/4716.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001416&mimeType=html&fmt=ahah

References

  1. Atlas R. M..( 1993;). Handbook of Microbiological Media. Edited by Parks L. C.. Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E..( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol100:221–230. [CrossRef][PubMed]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A..( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem12:133–142. [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J..( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol17:368–376. [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J..( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution39:783–791. [CrossRef]
    [Google Scholar]
  6. Fitch W. M..( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool20:406–416. [CrossRef]
    [Google Scholar]
  7. Funke G., Stubbs S., Altwegg M., Carlotti A., Collins M. D..( 1994;). Turicella otitidis gen. nov., sp. nov., a coryneform bacterium isolated from patients with otitis media. . Int J Syst Bacteriol44:270–273. [CrossRef][PubMed]
    [Google Scholar]
  8. Goodfellow M., Jones A. L..( 2012;). Order V. Corynebacteriales ord. nov. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,vol 5, part A pp. 235–509. Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K., Ludwig W., Whitman W. B.. New York:: Springer;.[CrossRef]
    [Google Scholar]
  9. Guo L., Tuo L., Habden X., Zhang Y. Q., Liu J. M., Jiang Z. K., Liu S. W., Dilbar T., Sun C. H..( 2015;). Allosalinactinospora lopnorensis gen. nov., sp. nov., a new member of the family Nocardiopsaceae isolated from soil. . Int J Syst Evol Microbiol65:206–213. [CrossRef][PubMed]
    [Google Scholar]
  10. Hall T. A..( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Window 95/98/NT. . Nucleic Acids Symp Ser41:95–98.
    [Google Scholar]
  11. Hasegawa T., Takizawa M., Tanida S..( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol29:319–322. [CrossRef]
    [Google Scholar]
  12. Jurado V., Kroppenstedt R. M., Saiz-Jimenez C., Klenk H. P., Mouniée D., Laiz L., Couble A., Pötter G., Boiron P., Rodríguez-Nava V..( 2009;). Hoyosella altamirensis gen. nov., sp. nov., a new member of the order Actinomycetales isolated from a cave biofilm. . Int J Syst Evol Microbiol59:3105–3110. [CrossRef][PubMed]
    [Google Scholar]
  13. Kelly K. L..( 1964;). Inter-Society Color Council‒National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  14. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol62:716–721. [CrossRef][PubMed]
    [Google Scholar]
  15. Kimura M..( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16:111–120. [CrossRef][PubMed]
    [Google Scholar]
  16. Kimura M..( 1983;). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  17. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A..( (editors)) ( 1992;). International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  18. Li W.-J., Xu P., Schumann P., Zhang Y.-Q., Pukall R., Xu L.-H., Stackebrandt E., Jiang C.-L..( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol57:1424–1428. [CrossRef]
    [Google Scholar]
  19. Magee C. M., Rodeheaver G., Edgerton M. T., Edlich R. F..( 1975;). A more reliable gram staining technic for diagnosis of surgical infections. . Am J Surg130:341–346. [CrossRef]
    [Google Scholar]
  20. Marmur J..( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol3:IN1–208. [CrossRef]
    [Google Scholar]
  21. Marmur J., Doty P..( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol5:109–118. [CrossRef][PubMed]
    [Google Scholar]
  22. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H..( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2:233–241. [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol4:406–425.[PubMed]
    [Google Scholar]
  24. Sasser M..( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, , MIDI Technical Note 101.. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  25. Shirling E. B., Gottlieb D..( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol16:313–340. [CrossRef]
    [Google Scholar]
  26. Staneck J. L., Roberts G. D..( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol28:226–231.[PubMed]
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S..( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol28:2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  28. Uchida K., Kudo T., Suzuki K., Nakase T..( 1999;). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. . J Gen Appl Microbiol45:49–56. [CrossRef][PubMed]
    [Google Scholar]
  29. Wang Y.-N., Chi C.-Q., Cai M., Lou Z.-Y., Tang Y.-Q., Zhi X.-Y., Li W.-J., Wu X.-L., Du X..( 2010;). Amycolicicoccus subflavus gen. nov., sp. nov., an actinomycete isolated from a saline soil contaminated by crude oil. . Int J Syst Evol Microbiol60:638–643. [CrossRef][PubMed]
    [Google Scholar]
  30. Wayne L., Brenner D., Colwell R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al.( 1987;). International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37:463–464.[CrossRef]
    [Google Scholar]
  31. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J..( 1983;). Numerical classification of Streptomyces and related genera. . J Gen Microbiol129:1743–1813. [CrossRef][PubMed]
    [Google Scholar]
  32. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L..( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. . Int J Syst Evol Microbiol55:1149–1153. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001416
Loading
/content/journal/ijsem/10.1099/ijsem.0.001416
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error