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Abstract

A pale-yellowish bacterium, strain KWS-1, was isolated from seawater during a study of the bacterial diversity of the marine environment of the Kanyakumari coastal region of the Bay of Bengal, India, and was studied by using a polyphasic taxonomic approach. Strain KWS-1 had morphological and chemotaxonomic properties (cell-wall diamino acid, menaquinone and fatty acid profile) consistent with its classification in the genus . Phylogenetic analysis of the 16S rRNA gene sequence showed that strain KWS-1 was related most closely to JCM 17781, followed by DSM 23186, JCM 19356, JCM 11609 and JCM 11608 (98.45, 98.24, 98.12, 98.10 and 98.10 % similarity, respectively), whereas the sequence similarity values with respect to the other species with validly published names were between 97.4 and 94.2 %. However, the DNA–DNA hybridization values between strain KWS-1 and the five most closely related species were less than the threshold value for species discrimination. The major lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine and the major quinone was menaquinone MK-7. The DNA G+C content of strain KWS-1 was 71.8 mol%. The above data in combination with the phenotypic distinctiveness of strain KWS-1 from other reference strains clearly indicate that the strain represents a novel species, for which the name sp. nov. is proposed. The type strain is KWS-1 (=MTCC 11836=DSM 28796=JCM 30059).

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2016-11-01
2020-08-11
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