1887

Abstract

Several fast- to intermediate-growing, acid-fast, scotochromogenic bacteria were isolated from pitcher waters in Minnesota sphagnum peat bogs. Two strains (DL734 and DL739) were among these isolates. On the basis of 16S rRNA gene sequences, the phylogenetic positions of both strains is in the genus with no obvious relation to any characterized type strains of mycobacteria. Phenotypic characterization revealed that neither strain was similar to the type strains of known species of the genus in the collective properties of growth, pigmentation or fatty acid composition. Strain DL734 grew at temperatures between 28 and 32 °C, was positive for 3-day arylsulfatase production, and was negative for Tween 80 hydrolysis, urease and nitrate reduction. Strain DL739 grew at temperatures between 28 and 37 °C, and was positive for Tween 80 hydrolysis, urea, nitrate reduction and 3-day arylsulfatase production. Both strains were catalase-negative while only DL739 grew with 5 % NaCl. Fatty acid methyl ester profiles were unique for each strain. DL739 showed an ability to survive at 8 °C with little to no cellular replication and is thus considered to be psychrotolerant. Therefore, strains DL734 and DL739 represent two novel species of the genus with the proposed names sp. nov. and sp. nov., respectively. The type strains are DL734 (=JCM 30395=NCCB 100519) and DL739 (=JCM 30396=NCCB 100520), respectively.

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2016-11-01
2019-12-11
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