1887

Abstract

A red-pigmented, Gram-stain-negative, rod-shaped, aerobic bacterium, designated strain CCM 8646, was isolated from stone fragments in James Ross Island, Antarctica. Strain CCM 8646 was able to grow from 10 to 40 °C, in the presence of up to 1 % (w/v) NaCl and at pH 7.0–11.0. Analysis of the 16S rRNA gene sequence placed strain CCM 8646 in the genus with the closest relative being H359 (97.07 % 16S rRNA gene sequence similarity). The digital DNA–DNA hybridization values between strain CCM 8646 and H359 were low (21.30±2.34 %). The major quinone was menaquinone MK-7. The polar lipids comprised phosphatidylethanolamine, an unknown aminoglycolipid and six unknown polar lipids. The G+C content of strain CCM 8646 was 51.54 mol%. On the basis of phenotypic, chemotaxonomic and genotyping results, strain CCM 8646 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is CCM 8646 (=LMG 29438).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001364
2016-11-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/11/4401.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001364&mimeType=html&fmt=ahah

References

  1. Abaydulla G., Luo X., Shi J., Peng F., Liu M., Wang Y., Dai J., Fang C.. 2012; Rufibacter tibetensis gen. nov., sp. nov., a novel member of the family Cytophagaceae isolated from soil. Antonie Van Leeuwenhoek101:725–731 [CrossRef][PubMed]
    [Google Scholar]
  2. Altenburgera P., Kämpferb P., Makristathisc A., Lubitza W., Bussea H.-J.. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol47:39–52 [CrossRef]
    [Google Scholar]
  3. Atlas R. M.. 2010; Handbook of Microbiological Media, 4th edn. Washington, D.C.: ASM Press;[CrossRef]
    [Google Scholar]
  4. Barrow G. I., Feltham R. K. A.. 1993; Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Great Britain: Cambridge University Press;[CrossRef]
    [Google Scholar]
  5. CLSI 2015; Performance Standards for Antimicrobial Susceptibility Testing Twenty-Fifth Informational Supplement (M100-S25) vol. 35, No. 3
    [Google Scholar]
  6. Da X., Jiang F., Chang X., Ren L., Qiu X., Kan W, Deng S., Fang C., Zhang Y. et al. 2015; Pedobacter ardleyensis sp. nov., isolated from soil in Antarctica. Int J Syst Evol Microbiol65:3841–3846[CrossRef]
    [Google Scholar]
  7. Edwards U., Rogall T., Blöcker H., Emde M., Böttger E. C.. 1989; Isolation and direct complete nucleotide determination of entire genes. characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res17:7843–7853 [CrossRef][PubMed]
    [Google Scholar]
  8. EUCAST 2015; European Committee on Antimicrobial Susceptibility Testing. EUCAST Clinical Breakpoints – Bacteria (Version 5.0;). http://www.eucast.org
  9. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M.. 2007; DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol57:81–91 [CrossRef][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  11. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  12. Kosina M., Barták M., Mašlaňová I., Pascutti A. V., Šedo O., Lexa M., Sedláček I.. 2013; Pseudomonas prosekii sp. nov., a novel psychrotrophic bacterium from Antarctica. Curr Microbiol67:637–646 [CrossRef][PubMed]
    [Google Scholar]
  13. Lagesen K., Hallin P. F., Rødland E., Stærfeldt H. H., Rognes T., Ussery D. W.. 2007; RNAmmer: consistent annotation of rRNA genes in genomic sequences. Nucleic Acids Res35:3100–3108[CrossRef]
    [Google Scholar]
  14. Liu Q., Liu H. C., Zhang J. L., Zhou Y. G., Xin Y. H.. 2016; Rufibacter glacialis sp. nov., a psychrotolerant bacterium isolated from glacier soil. Int J Syst Evol Microbiol66:315–318 [CrossRef][PubMed]
    [Google Scholar]
  15. Meier-Kolthoff J. P., Auch A. F., Klenk H. P., Göker M.. 2013; Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics14:60 [CrossRef][PubMed]
    [Google Scholar]
  16. Polkade A. V., Ramana V. V., Joshi A., Pardesi L., Shouche Y. S.. 2015; Rufibacter immobilis sp. nov., isolated from a high-altitude saline lake. Int J Syst Evol Microbiol65:1592–1597[CrossRef]
    [Google Scholar]
  17. Richter M., Rosselló-Móra R.. 2009; Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A106:19126–19131 [CrossRef][PubMed]
    [Google Scholar]
  18. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
    [Google Scholar]
  19. Sedláček I., Kwon S.-W., Černohlávková J., Mašlanˇová I., Kýrová K., Švec P., Holochová P., Busse H.-J., Švec P. et al. 2016; Aquitalea pelogenes sp. nov., isolated from mineral peloid. Int J Syst Evol Microbiol66:962–967[CrossRef]
    [Google Scholar]
  20. Stolz A., Busse H.-J., Kämpfer P.. 2007; Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol57:572–576 [CrossRef][PubMed]
    [Google Scholar]
  21. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  22. Tindall B. J.. 1990a; Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett66:199–202 [CrossRef]
    [Google Scholar]
  23. Tindall B. J.. 1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol13:128–130 [CrossRef]
    [Google Scholar]
  24. Zhang Z. D., Gu M. Y., Zhu J., Li S. H., Zhang L. J., Xie Y. Q., Shi Y. H., Wang W., Li W. J.. 2015; Rufibacter roseus sp. nov., isolated from radiation-polluted soil. Int J Syst Evol Microbiol65:1572–1577 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001364
Loading
/content/journal/ijsem/10.1099/ijsem.0.001364
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error