1887

Abstract

A yellow, Gram-staining-positive, aerobic, rod-shaped and non-motile bacterial strain, designated CJ663, was isolated from the tidal flat sediment in Ganghwa-do, South Korea. Strain CJ663 grew optimally on R2A at 30 °C and pH 7.0 and did not require NaCl for growth. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain CJ663 belonged to the genus within the family and was most closely related to KACC 17467 (98.3 % similarity), followed by KACC 17917 (97.4 %). DNA–DNA relatedness levels of strain CJ663 were 42.9 % to KACC 17467 and 48.6 % to KACC 17917. The major isoprenoid quinone was menaquinone 7 (MK-7). The predominant polar lipids were phosphatidylethanolamine, aminophospholipid and two unidentified lipids. The major cellular fatty acids of strain CJ663 were iso-C, anteiso-C and iso-C 3-OH. The G+C content of the genomic DNA was 47.7 mol%. On the basis of data from this polyphasic taxonomic study, strain CJ663 represents a novel species in the genus , for which name sp. nov. is proposed; the type strain is CJ663 (=KACC 18874=JCM 31431).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001349
2016-11-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/11/4310.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001349&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y., Holmes B., Flavobacteri S. T.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  2. Collins M. D. 1985 Chemical Methods in Bacterial Systematics London: Academic Press;
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane-filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  6. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 , pp. 21–132 Edited by Munro H. N. New York: Academic Press; [CrossRef]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  8. Kim W. H., Lee S., Ahn T. Y. 2014; Flavihumibacter cheonanensis sp. nov., isolated from sediment of a shallow stream. Int J Syst Evol Microbiol 64:3235–3239 [View Article][PubMed]
    [Google Scholar]
  9. Kumar S., Stecher G., Tamura K. 2016; mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  10. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  11. Lee H. J., Jeong S. E., Cho M. S., Kim S., Lee S. S., Lee B. H., Jeon C. O. 2014; Flavihumibacter solisilvae sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 64:2897–2901 [View Article][PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  13. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  15. Spormann A. M. 1999; Gliding motility in bacteria: insights from studies of Myxococcus xanthus. Microbiol Mol Biol Rev 63:621–641[PubMed]
    [Google Scholar]
  16. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  17. Tindall B. J. 1990a; A comparative study of the lipid composition of saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  18. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I, Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  20. Zhang N. N., Qu J. H., Yuan H. L., Sun Y. M., Yang J. S. 2010; Flavihumibacter petaseus gen. nov., sp. nov., isolated from soil of a subtropical rainforest. Int J Syst Evol Microbiol 60:1609–1612 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001349
Loading
/content/journal/ijsem/10.1099/ijsem.0.001349
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error