1887

Abstract

A Gram-staining-negative, non-motile, orange-coloured and rod-shaped aerobic bacterium, designated W18RD, was isolated from the alpine meadow soil of the Tibetan plateau. Phylogenetic analysis based on 16S rRNA gene sequences positioned strain W18RD as a representative of a novel species under the genus which was most closely related to DSM 13665 with a sequence similarity level of 97.14 %. Meanwhile, it also had a high level of sequence similarity with DSM 25432 (96.51 %), CGMCC 1.10206 (96.43 %) and DSM 24164 (96.26 %). The G+C content of the genomic DNA of the type strain W18RD was 66.4mol%. DNA–DNA relatedness for the type strain W18RD with respect to its closest phylogenetic relative DSM 13665was 21.54±1.2 %. Major cellular fatty acids in strain W18RD were C 7 and/or C 6 (48.12 %), C 7 and/or C 6 (21.98 %) and C 2-OH (14.93 %), with ubiquinone-10 (Q-10) as the predominant respiratory quinone. The polar lipid profile of the strain consisted of phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, diphosphatidylglycerol and two unknown lipids. Based on the evidence from a combination of phenotypic, taxonomic and phylogenetic analyses, strain W18RD represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W18RD (=CGMCC 1.15645=DSM 103336).

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2016-10-01
2021-10-23
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