1887

Abstract

Recently, two novel species of were detected in the Australian paralysis tick, , by 16S rRNA gene metabarcoding. Analysis of these sequences suggested that these novel organisms are closely related to the genus ‘ Neoehrlichia’. In this study, phylogenetic analysis of 16S rRNA (1264 bp), (1047 bp) and (561 bp) gene sequences, and concatenated (2872 bp) sequences, all concur that these novel species belong in the genus ' Neoehrlichia' and are most closely related to, but distinct from the only other recognised members of this genus, ‘ Neoehrlichia mikurensis’ and ‘ Neoehrlichia lotoris’. Based on their unique molecular signature, we propose to designate these species ‘ Neoehrlichia australis’ (reference strain HT41) and ‘ Neoehrlichia arcana’ (reference strain HT94). Identical ‘ Neoehrlichia australis’ 16S rRNA, and sequences were detected in 34/391 (8.7 %) individual ticks, and sequences were most similar to ‘ Neoehrlichia lotoris’ (96.2 %, 83.1 % and 67.2 %, respectively) and ‘ Neoehrlichia mikurensis’ (96.2 %, 84 % and 68.4 % respectively). Likewise, identical ‘ Neoehrlichia arcana’ 16S rRNA, and sequences were detected in 12/391 (3.1 %) ticks, and sequences were most similar to ‘ Neoehrlichia lotoris’ (98.5 %, 88.7 % and 79.3 %, respectively) and ‘ Neoehrlichia mikurensis’ (96.3 %, 84 % and 67.4 % respectively). These new species are the first (except spp.) to be found to be endemic to Australia. The pathogenic consequences of these organisms are yet to be determined.

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2016-10-01
2019-12-11
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