1887

Abstract

A novel actinobacterial strain, B2-12, was isolated from soil of a crater lake, Baekrokdam, Hallasan, Jeju, Republic of Korea. Cells of strain B2-12 were Gram-stain-positive, non-motile, non-spore-forming and coccoid to short-rod-shaped. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain B2-12 belonged to the genus and shared highest sequence similarity with ‘’ KIS31-44 (98.4 %). The predominant isoprenoid quinone was MK-8(H). The major fatty acids of strain B2-12 were C (7 and/or 6), summed feature 9 (iso-C 9 and/or C 10-methyl), C 10-methyl, iso-C, C and C 6. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. It contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the polar lipids. The DNA G+C content was 67.0 mol%. Based on phylogenetic, chemotaxonomic and phenotypic data, 16S rRNA gene sequence comparisons and DNA–DNA hybridization data, strain B2-12 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is B2-12 (=KCTC 39748=NRRL B-65313=DSM 100725).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001340
2016-10-01
2020-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/4231.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001340&mimeType=html&fmt=ahah

References

  1. Ahn J. H., Lim J. M., Kim S. J., Song J., Kwon S. W., Weon H. Y.. 2014; Nocardioides paucivorans sp. nov. isolated from soil. J Microbiol52:990–994 [CrossRef][PubMed]
    [Google Scholar]
  2. Euzéby J. P.. 1997; List of bacterial names with standing in nomenclature: a folder available on the internet. Int J Syst Evol Microbiol47:590–592 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39:224–229 [CrossRef]
    [Google Scholar]
  4. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  6. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20:406–416 [CrossRef]
    [Google Scholar]
  7. Gomori G.. 1955; Preparation of buffers for use in enzyme studies. Methods Enzymol1:138–146[CrossRef]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R.. 1969; Evolution of protein molecules. In Mammalian Protein Metabolismvol. 3 pp21–132 Edited by Munro H. N.. New York, NY: Academic Press;[CrossRef]
    [Google Scholar]
  9. Kim J. K., Liu Q. M., Park H. Y., Kang M. S., Kim S. C., Im W. T., Yoon M. H.. 2013; Nocardioides panaciterrulae sp. nov., isolated from soil of a ginseng field, with ginsenoside converting activity. Antonie Van Leeuwenhoek103:1385–1393 [CrossRef][PubMed]
    [Google Scholar]
  10. Komagata K., Suzuki K.-I.. 1987; Lipid and cell-wallanalysis in bacterial systematics. In Methods in Microbiologyvol. 19 pp.182–190 Edited by Colwell R. R., Grigorova R.. London: Academic Press;
    [Google Scholar]
  11. Lee K. C., Kim K. K., Eom M. K., Kim M. J., Lee J.-S.. 2011; Fontibacillus panacisegetis sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol61:369–374 [CrossRef][PubMed]
    [Google Scholar]
  12. Lányí B.. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol19:1–67[CrossRef]
    [Google Scholar]
  13. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol27:104–117 [CrossRef]
    [Google Scholar]
  14. O'Donnell A. G., Goodfellow M., Minnikin D. E.. 1982; Lipids in the classification of Nocardioides: reclassification of Arthrobacter simplex (Jensen) lochhead in the genus Nocardioides (Prauser) emend. O'Donnell et al. as Nocardioides simplex comb. nov. Arch Microbiol133:323–329 [CrossRef][PubMed]
    [Google Scholar]
  15. Prauser H.. 1976; Nocardioides, a new genus of the order Actinomycetales. Int J Syst Bacteriol26:58–65 [CrossRef]
    [Google Scholar]
  16. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  17. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
    [Google Scholar]
  18. Schleifer K. H., Kandler O.. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev36:407–477[PubMed]
    [Google Scholar]
  19. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  20. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  21. Urzì C., Salamone P., Schumann P., Stackebrandt E.. 2000; Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. Int J Syst Evol Microbiol50:529–536 [CrossRef][PubMed]
    [Google Scholar]
  22. Yoon J.-H., Kang S.-J., Lee C.-H., Oh T.-K.. 2007; Nocardioides insulae sp. nov., isolated from soil. Int J Syst Evol Microbiol57:136–140 [CrossRef][PubMed]
    [Google Scholar]
  23. Zhang J. Y., Liu X. Y., Liu S. J.. 2009; Nocardioides terrae sp. nov., isolated from forest soil. Int J Syst Evol Microbiol59:2444–2448 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001340
Loading
/content/journal/ijsem/10.1099/ijsem.0.001340
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error