1887

Abstract

A novel actinobacterial strain, B2-12, was isolated from soil of a crater lake, Baekrokdam, Hallasan, Jeju, Republic of Korea. Cells of strain B2-12 were Gram-stain-positive, non-motile, non-spore-forming and coccoid to short-rod-shaped. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain B2-12 belonged to the genus and shared highest sequence similarity with ‘’ KIS31-44 (98.4 %). The predominant isoprenoid quinone was MK-8(H). The major fatty acids of strain B2-12 were C (7 and/or 6), summed feature 9 (iso-C 9 and/or C 10-methyl), C 10-methyl, iso-C, C and C 6. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. It contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the polar lipids. The DNA G+C content was 67.0 mol%. Based on phylogenetic, chemotaxonomic and phenotypic data, 16S rRNA gene sequence comparisons and DNA–DNA hybridization data, strain B2-12 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is B2-12 (=KCTC 39748=NRRL B-65313=DSM 100725).

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2016-10-01
2021-10-27
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