1887

Abstract

An endophytic actinobacterial strain, designated py1292, was isolated from the root of Smith var. collected from Yunnan province, China, and subjected to a polyphasic taxonomic characterization. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate in the family and clustered with (showing the highest similarity of 99.1 %). DNA–DNA relatedness between strain py1292 and YIM 45900 was 45.6±3.2 % (reciprocal 47.8±3.6 %). The novel isolate was found to be a Gram-staining-positive rod, catalase- and oxidase-positive. It grew at pH 6.0–8.0, with 0–9 % NaCl and at 20–45 ºC, optimally at pH 7.0, with 0–3 % NaCl and at 28 ºC. The predominant menaquinone was MK-8(H), while the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unknown phospholipids and two unknown polar lipids. The major cellular fatty acids were iso-C, iso-C, iso-C and anteiso-C. The whole-cell hydrolysates contained mannose, ribose, rhamnose, glucose and galactose. The peptidoglycan contained alanine, glycine, serine, aspartic acid, glutamic acid and lysine. The genomic DNA G+C content was determined to be 65.6 mol%. Phylogenetic, phenotypic and chemotaxonomic data (especially the same peptidoglycan type) showed that strain py1292 should be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is py1292 (=KCTC 39612=LMG 29070).

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2016-10-01
2020-01-20
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