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Abstract

A Gram-reaction-negative, heterotrophic, marine bacterium, designated strain SPT1, was isolated from an aged seawater sample which was collected from the shallow coastal region of Nanya, Keelung, Taiwan and stored at room temperature for more than 7 years. Strain SPT1 was a motile rod which exhibited monotrichous flagellation. It required NaCl for growth and exhibited optimal growth at 30–35 °C, 1–3 % NaCl and pH 7–8. The strain was a strictly aerobic bacterium, incapable of anaerobic growth by nitrate reduction or denitrification, or by fermenting glucose or other carbohydrates. Cellular fatty acids were dominated by Cω7 and/or Cω6 (23.4 %), Cω8 (18.1 %), C (8.5 %), Cω7 (8.4 %) and C 3-OH (6.3 %). The predominant isoprenoid quinone was Q-8. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and phosphatidic acid. The DNA G+C content was 57.9 mol%. Phylogeny based on 16S rRNA gene sequences showed that strain SPT1 formed a distinct species-level lineage within the genus of the class and shared sequence similarities of 94.4–96.2 % with and the only two species of the genus with validly published names. The 16S rRNA gene sequence similarities between strain SPT1 and other species were less than 93.1 %. Polyphasic taxonomic data obtained in this study indicated that strain SPT1 could be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SPT1 (=JCM 31012=BCRC 80916).

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2016-10-01
2020-01-29
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