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Abstract

A Gram-reaction-negative, heterotrophic, marine bacterium, designated strain SPT1, was isolated from an aged seawater sample which was collected from the shallow coastal region of Nanya, Keelung, Taiwan and stored at room temperature for more than 7 years. Strain SPT1 was a motile rod which exhibited monotrichous flagellation. It required NaCl for growth and exhibited optimal growth at 30–35 °C, 1–3 % NaCl and pH 7–8. The strain was a strictly aerobic bacterium, incapable of anaerobic growth by nitrate reduction or denitrification, or by fermenting glucose or other carbohydrates. Cellular fatty acids were dominated by Cω7 and/or Cω6 (23.4 %), Cω8 (18.1 %), C (8.5 %), Cω7 (8.4 %) and C 3-OH (6.3 %). The predominant isoprenoid quinone was Q-8. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and phosphatidic acid. The DNA G+C content was 57.9 mol%. Phylogeny based on 16S rRNA gene sequences showed that strain SPT1 formed a distinct species-level lineage within the genus of the class and shared sequence similarities of 94.4–96.2 % with and the only two species of the genus with validly published names. The 16S rRNA gene sequence similarities between strain SPT1 and other species were less than 93.1 %. Polyphasic taxonomic data obtained in this study indicated that strain SPT1 could be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SPT1 (=JCM 31012=BCRC 80916).

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2016-10-01
2021-10-24
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References

  1. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  2. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  3. Graeber I., Kaesler I., Borchert M. S., Dieckmann R., Pape T., Lurz R., Nielsen P., von Döhren H., Michaelis W., Szewzyk U. 2008; Spongiibacter marinus gen. nov., sp. nov., a halophilic marine bacterium isolated from the boreal sponge Haliclona sp. 1. Int J Syst Evol Microbiol 58:585–590 [View Article][PubMed]
    [Google Scholar]
  4. Hwang C. Y., Cho B. C. 2009; Spongiibacter tropicus sp. nov., isolated from a Synechococcus culture. Int J Syst Evol Microbiol 59:2176–2179 [View Article][PubMed]
    [Google Scholar]
  5. Jang G. I., Hwang C. Y., Choi H. G., Kang S. H., Cho B. C. 2011; Description of Spongiibacter borealis sp. nov., isolated from Arctic seawater, and reclassification of Melitea salexigens Urios et al. 2008 as a later heterotypic synonym of Spongiibacter marinus Graeber et al. 2008 with emended descriptions of the genus Spongiibacter and Spongiibacter marinus . Int J Syst Evol Microbiol 61:2895–2900 [View Article][PubMed]
    [Google Scholar]
  6. Jean W. D., Shieh W. Y., Chiu H. H. 2006; Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae . Int J Syst Evol Microbiol 56:899–905 [View Article][PubMed]
    [Google Scholar]
  7. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 , pp. 21–132 Edited by Munro H. N. New York, NY: Academic Press; [CrossRef]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  9. Lin Y. T., Shieh W. Y. 2006; Zobellella denitrificans gen. nov., sp. nov. and Zobellella taiwanensis sp. nov., denitrifying bacteria capable of fermentative metabolism. Int J Syst Evol Microbiol 56:1209–1215 [View Article][PubMed]
    [Google Scholar]
  10. Lo N., Kang H. J., Jeon C. O. 2014; Zhongshania aliphaticivorans sp. nov., an aliphatic hydrocarbon-degrading bacterium isolated from marine sediment, and transfer of Spongiibacter borealis Jang et al. 2011 to the genus Zhongshania as Zhongshania borealis comb. nov. Int J Syst Evol Microbiol 64:3768–3774 [View Article][PubMed]
    [Google Scholar]
  11. Nawrocki E. P., Kolbe D. L., Eddy S. R. 2009; Infernal 1.0: inference of RNA alignments. Bioinformatics 25:1335–1337 [View Article][PubMed]
    [Google Scholar]
  12. Nishijima M., Araki-Sakai M., Sano H. 1997; Identification of isoprenoid quinones by frit-FAB liquid chromatography–mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods 28:113–122 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  14. Sasser M. 2001 Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  15. Shieh W. Y., Chen A. L., Chiu H. H. 2000; Vibrio aerogenes sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production. Int J Syst Evol Microbiol 50:321–329 [View Article][PubMed]
    [Google Scholar]
  16. Shieh W. Y., Liu T. Y., Lin S. Y., Jean W. D., Chen J. S. 2008; Simiduia agarivorans gen. nov., sp. nov., a marine, agarolytic bacterium isolated from shallow coastal water from Keelung, Taiwan. Int J Syst Evol Microbiol 58:895–900 [View Article][PubMed]
    [Google Scholar]
  17. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  18. Urios L., Agogué H., Intertaglia L., Lesongeur F., Lebaron P. 2008; Melitea salexigens gen. nov., sp. nov., a gammaproteobacterium from the Mediterranean sea. Int J Syst Evol Microbiol 58:2479–2483 [View Article][PubMed]
    [Google Scholar]
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