1887

Abstract

A Gram-staining-negative, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated WG4, was isolated from soil of the Tianmen Mountain located in Hunan province, PR China. 16S rRNA gene sequence analysis showed that the strain belongs to the genus Chryseobacterium in the family Flavobacteriaceae , with 97.4 % and 97.1 % sequence identities to Chryseobacterium anthropi NF 1366 and Chryseobacterium haifense H38, respectively. In comparison with the other strains representing the genus Chryseobacterium , the 16S rRNA gene sequence identities were less than 97.0 %. The DNA–DNA relatedness values were 63.3 % (±1) between C. anthropi NF 1366 and strain WG4 and 62.7 % (±2) between C. haifense DSM 19056 and strain WG4. The DNA G+C content of strain WG4 was 37.7 mol%. The predominant fatty acids of strain WG4 were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, three unidentified lipids and two unidentified aminolipids. The major menaquinone of strain WG4 was menaquinone 6. Strain WG4 showed some unique physiological and biochemical characteristics, such as being negative for gelatin hydrolysis, and valine arylamidase and α-glucosidase activity, and positive for acid production from cellobiose. Based on the differentiating phylogenetic inference and biochemical data, strain WG4 represents a novel species, for which the name Chryseobacterium montanum sp. nov. is proposed, with the type strain WG4 (=KCTC 52204=CCTCC AB 2016058).

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2016-10-01
2019-10-21
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