1887

Abstract

A novel Gram-stain-negative, aerobic and rod-shaped bacterial strain, designated 65, was isolated from surface-sterilized root tissue of maize, collected from Fangshan District of Beijing, People’s Republic of China, and was subjected to a taxonomic study by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 65 belonged to the genus and had highest 16S rRNA gene sequence similarity to CGMCC 1.12969 (99.1 %), CGMCC 1.6375 (98.8 %), DSM 18053 (98.6 %) and KCTC 22481 (98.6 %). However, the new isolate exhibited relatively low levels of DNA–DNA relatedness with respect to CGMCC 1.12969 (18.2±1.3 %), CGMCC 1.6375 (14.2±2.0 %), DSM 18053 (14.1±2.0 %) and KCTC 22481 (13.8±0.6 %). The predominant respiratory quinone was menaquinone-7 (MK-7) and the major cellular fatty acids were summed feature 3 (C 7 and/or iso-C 2-OH), iso-C , iso-C 3-OH, C 5, iso-C 3-OH, C 3-OH and C. The polar lipid profile of strain 65 revealed the presence of phosphatidylethanolamine, four aminolipids and two unidentified phospholipids. The DNA G+C content was 46.6 mol%. The results of physiological and biochemical tests and the differences in the fatty acid profiles allowed the clear phenotypic differentiation of strain 65 from closely related species of the genus Strain 65 thus represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 65 (=CGMCC 1.15288=DSM 100786).

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2016-10-01
2020-03-28
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