1887

Abstract

Actinomycete strain CMU-AB204 was isolated from oil palm rhizosphere soil collected in Chiang Mai University (Chiang Mai, Thailand). Based on morphological and chemotaxonomic characteristics, the organism was considered to belong to the genus . Whole cell-wall hydrolysates consisted of -diaminopimelic acid, glucose, ribose and galactose. The predominant menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-8(H). The fatty acid profile contained iso-C, iso-C and anteiso-C as major components. The principal phospholipids detected were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content of strain CMU-AB204 was 70.9 mol%. Based on 16S rRNA gene sequence similarity, strain CMU-AB204 was closely related to JCM 4546 (98.7 %), NBRC 12884 (98.5 %), CGMCC 4.1662 (98.5 %), JCM 4905 (98.4 %) and JCM 16958 (98.3 %). Phylogenetic trees revealed that the new strain had a distinct taxonomic position from closely related type strains of the genus . Spiny to hairy spores clearly differentiated strain CMU-AB204 from the five most closely related species, which produced smooth spores. On the basis of evidence from this polyphasic study, it is proposed that strain CMU-AB204 represents a novel species of the genus , namely sp. nov. The type strain is CMU-AB204 (=JCM 31289=TBRC 1999).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001298
2016-10-01
2021-07-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/3983.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001298&mimeType=html&fmt=ahah

References

  1. Bérdy J. 2005; Bioactive microbial metabolites. J Antibiot (Tokyo) 58:1–26 [View Article][PubMed]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
    [Google Scholar]
  3. Felsenstein J. 1981; Evolutionary trees from dna sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  5. Hamdali H., Virolle M. J., von Jan M., Spröer C., Klenk H. P., Ouhdouch Y. 2011; Streptomyces youssoufiensis sp. nov., isolated from a Moroccan phosphate mine. Int J Syst Evol Microbiol 61:1104–1108 [View Article][PubMed]
    [Google Scholar]
  6. Hatano K., Nishii T., Kasai H. 2003; Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA-DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. rev. Int J Syst Evol Microbiol 53:1519–1529 [View Article][PubMed]
    [Google Scholar]
  7. Kämpfer P. 2012; Genus I. Streptomyces Waksman and Henrici 1943, 339AL emend. Witt and Stackebrandt 1990, 370 emend. Wellington, Stackebrandt, Sanders, Wolstrup and Jorgensen 1992, 159. In Bergey’s Manual of Systematic Bacteriology, Part B, 2nd edn. vol. 5 pp. 1455–1767 . Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K.-I., Ludwig W., Whitman W. B. New York: Springer; [CrossRef]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  10. Kluge A. G., Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [View Article]
    [Google Scholar]
  11. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp. 173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  12. Küster E., Williams S. T. 1964; Selection of media for isolation of streptomycetes. Nature 202:928–929 [View Article]
    [Google Scholar]
  13. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. 2007; clustal w and clustal x version 2.0. Bioinformatics 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  14. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
    [Google Scholar]
  15. Li W., Lanoot B., Zhang Y., Vancanneyt M., Swings J., Liu Z. 2002; Streptomyces scopiformis sp. nov., a novel streptomycete with fastigiate spore chains. Int J Syst Evol Microbiol 52:1629–1633 [View Article][PubMed]
    [Google Scholar]
  16. Locci R. 1989; Streptomycetes and related genera. In Bergey’s Manual of Systematic Bacteriology vol. 4 pp. 2451–2452 . Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  17. Mikami H., Ishida Y. 1983; Post-column fluorometric detection of reducing sugarin high-performance liquid chromatography using arginine. Bunseki . Kagaku 32:E207E210
    [Google Scholar]
  18. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Meth 2:233–241 [View Article]
    [Google Scholar]
  19. Mohammadipanah F., Hamedi J., Spröer C., Rohde M., Montero-Calasanz M. C., Klenk H. P. 2014; Streptomyces zagrosensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 64:3434–3440 [View Article][PubMed]
    [Google Scholar]
  20. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. 1999; Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49:1761–1767 [View Article][PubMed]
    [Google Scholar]
  21. Raeder U., Broda P. 1985; Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1:17–20 [View Article]
    [Google Scholar]
  22. Rong X., Huang Y. 2012; Taxonomic evaluation of the Streptomyces hygroscopicus clade using multilocus sequence analysis and DNA-DNA hybridization, validating the MLSA scheme for systematics of the whole genus. Syst Appl Microbiol 35:7–18 [View Article][PubMed]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  24. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  25. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  26. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  27. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  28. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  29. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36 [View Article]
    [Google Scholar]
  30. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  31. Taylor H. D., Knoche L., Grauville W. C. 1958 Color Harmony Manual, 4th edn. Chicago: Container Corporation of America;
    [Google Scholar]
  32. Waksman S. A., Henrici A. T. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol 46:337–341[PubMed]
    [Google Scholar]
  33. Watve M. G., Tickoo R., Jog M. M., Bhole B. D. 2001; How many antibiotics are produced by the genus Streptomyces? . Arch Microbiol 176:386–390 [View Article][PubMed]
    [Google Scholar]
  34. Zhao X. Q., Li W. J., Jiao W. C., Li Y., Yuan W. J., Zhang Y. Q., Klenk H. P., Suh J. W., Bai F. W. 2009; Streptomyces xinghaiensis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 59:2870–2874 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001298
Loading
/content/journal/ijsem/10.1099/ijsem.0.001298
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error