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Abstract

Actinomycete strain CMU-AB204 was isolated from oil palm rhizosphere soil collected in Chiang Mai University (Chiang Mai, Thailand). Based on morphological and chemotaxonomic characteristics, the organism was considered to belong to the genus . Whole cell-wall hydrolysates consisted of -diaminopimelic acid, glucose, ribose and galactose. The predominant menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-8(H). The fatty acid profile contained iso-C, iso-C and anteiso-C as major components. The principal phospholipids detected were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content of strain CMU-AB204 was 70.9 mol%. Based on 16S rRNA gene sequence similarity, strain CMU-AB204 was closely related to JCM 4546 (98.7 %), NBRC 12884 (98.5 %), CGMCC 4.1662 (98.5 %), JCM 4905 (98.4 %) and JCM 16958 (98.3 %). Phylogenetic trees revealed that the new strain had a distinct taxonomic position from closely related type strains of the genus . Spiny to hairy spores clearly differentiated strain CMU-AB204 from the five most closely related species, which produced smooth spores. On the basis of evidence from this polyphasic study, it is proposed that strain CMU-AB204 represents a novel species of the genus , namely sp. nov. The type strain is CMU-AB204 (=JCM 31289=TBRC 1999).

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2016-10-01
2020-09-22
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References

  1. Bérdy J.. 2005; Bioactive microbial metabolites. J Antibiot (Tokyo)58:1–26 [CrossRef][PubMed]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  3. Felsenstein J.. 1981; Evolutionary trees from dna sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  5. Hamdali H., Virolle M. J., von Jan M., Spröer C., Klenk H. P., Ouhdouch Y.. 2011; Streptomyces youssoufiensis sp. nov., isolated from a Moroccan phosphate mine. Int J Syst Evol Microbiol61:1104–1108 [CrossRef][PubMed]
    [Google Scholar]
  6. Hatano K., Nishii T., Kasai H.. 2003; Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA-DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. rev. Int J Syst Evol Microbiol53:1519–1529 [CrossRef][PubMed]
    [Google Scholar]
  7. Kämpfer P.. 2012; Genus I. Streptomyces Waksman and Henrici 1943, 339AL emend. Witt and Stackebrandt 1990, 370 emend. Wellington, Stackebrandt, Sanders, Wolstrup and Jorgensen 1992, 159. In Bergey’s Manual of Systematic Bacteriology, Part B, 2nd edn.vol. 5 pp.1455–1767 . Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K.-I., Ludwig W., Whitman W. B.. New York: Springer;[CrossRef]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  9. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  10. Kluge A. G., Farris J. S.. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool18:1–32 [CrossRef]
    [Google Scholar]
  11. Kroppenstedt R. M.. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp.173–199 Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;
    [Google Scholar]
  12. Küster E., Williams S. T.. 1964; Selection of media for isolation of streptomycetes. Nature202:928–929 [CrossRef]
    [Google Scholar]
  13. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. 2007; clustal w and clustal x version 2.0. Bioinformatics23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  14. Lechevalier M. P., Lechevalier H. A.. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol20:435–443 [CrossRef]
    [Google Scholar]
  15. Li W., Lanoot B., Zhang Y., Vancanneyt M., Swings J., Liu Z.. 2002; Streptomyces scopiformis sp. nov., a novel streptomycete with fastigiate spore chains. Int J Syst Evol Microbiol52:1629–1633 [CrossRef][PubMed]
    [Google Scholar]
  16. Locci R.. 1989; Streptomycetes and related genera. In Bergey’s Manual of Systematic Bacteriology vol. 4 pp.2451–2452 . Edited by Williams S. T., Sharpe M. E., Holt J. G.. Baltimore: Williams & Wilkins;
    [Google Scholar]
  17. Mikami H., Ishida Y.. 1983; Post-column fluorometric detection of reducing sugarin high-performance liquid chromatography using arginine. Bunseki. Kagaku32:E207E210
    [Google Scholar]
  18. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Meth2:233–241 [CrossRef]
    [Google Scholar]
  19. Mohammadipanah F., Hamedi J., Spröer C., Rohde M., Montero-Calasanz M. C., Klenk H. P.. 2014; Streptomyces zagrosensis sp. nov., isolated from soil. Int J Syst Evol Microbiol64:3434–3440 [CrossRef][PubMed]
    [Google Scholar]
  20. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T.. 1999; Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol49:1761–1767 [CrossRef][PubMed]
    [Google Scholar]
  21. Raeder U., Broda P.. 1985; Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol1:17–20 [CrossRef]
    [Google Scholar]
  22. Rong X., Huang Y.. 2012; Taxonomic evaluation of the Streptomyces hygroscopicus clade using multilocus sequence analysis and DNA-DNA hybridization, validating the MLSA scheme for systematics of the whole genus. Syst Appl Microbiol35:7–18 [CrossRef][PubMed]
    [Google Scholar]
  23. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  24. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  25. Shirling E. B., Gottlieb D.. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol16:313–340 [CrossRef]
    [Google Scholar]
  26. Stackebrandt E., Ebers J.. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today33:152–155
    [Google Scholar]
  27. Staneck J. L., Roberts G. D.. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28:226–231[PubMed]
    [Google Scholar]
  28. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  29. Tamaoka J., Katayama-Fujimura Y., Kuraishi H.. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol54:31–36 [CrossRef]
    [Google Scholar]
  30. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  31. Taylor H. D., Knoche L., Grauville W. C.. 1958; Color Harmony Manual, 4th edn. Chicago: Container Corporation of America;
    [Google Scholar]
  32. Waksman S. A., Henrici A. T.. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol46:337–341[PubMed]
    [Google Scholar]
  33. Watve M. G., Tickoo R., Jog M. M., Bhole B. D.. 2001; How many antibiotics are produced by the genus Streptomyces?. Arch Microbiol176:386–390 [CrossRef][PubMed]
    [Google Scholar]
  34. Zhao X. Q., Li W. J., Jiao W. C., Li Y., Yuan W. J., Zhang Y. Q., Klenk H. P., Suh J. W., Bai F. W.. 2009; Streptomyces xinghaiensis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol59:2870–2874 [CrossRef][PubMed]
    [Google Scholar]
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