1887

Abstract

A novel sulfate-reducing bacterium, strain S606, was isolated from a sulfide sample collected at a depth of 2764 m from a deep-sea vent chimney wall in the Indian Ocean. Phylogenetic 16S rRNA gene sequence analyses placed strain S606 within the genus , with highest sequence similarity of 98.2 % to DSM 15286, followed by AT1325 (97.4 %). The average nucleotide identity (ANI) values between S606 and the two other type strains ( DSM 15286 and AT1325) were 79.2 % and 71.5 %, respectively. The digital DNA–DNA hybridization estimate values between S606 and these two type strains were 22.7±2.4 % and 18.1±2.3 %, respectively. Cells were Gram-stain-negative, anaerobic, motile rods (1–1.8×0.5–0.7 µm). The novel isolate grew at NaCl concentrations ranging from 1.5 to 4.5 % (optimum 2.5–3 %), from pH 5.5 to 8 (optimum 6.5–7.0) and at temperatures between 50 and 80 °C (optimum 65–70 °C). S606 grew chemolithoautotrophically in an H/CO atmosphere (80 : 20, v/v; 200 kPa), used sulfate as a terminal electron acceptor, but not sulfur, sulfite nor thiosulfate. The predominant fatty acids were C (24.2 %), summed feature 8 (Cω6 and/or C 7, 26.3 %), C (22.2 %) and Cω9 (9.2 %). The DNA G+C content of the chromosomal DNA was 43.1 mol%. The combined genotypic, chemotaxonomic and phenotypic traits show that S606 should be described as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S606 (=DSM 101864=MCCC 1A01871).

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2016-10-01
2024-12-14
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References

  1. Alain K., Rolland S., Crassous P., Lesongeur F., Zbinden M., le Gall C., Godfroy A., Page A., Juniper S. K. et al. 2003; Desulfurobacterium crinifex sp. nov., a novel thermophilic, pinkish-streamer forming, chemolithoautotrophic bacterium isolated from a Juan de Fuca Ridge hydrothermal vent and amendment of the genus Desulfurobacterium. Extremophiles 7:361–370 [View Article][PubMed]
    [Google Scholar]
  2. Alain K., Postec A., Grinsard E., Lesongeur F., Prieur D., Godfroy A. 2010; Thermodesulfatator atlanticus sp. nov., a thermophilic, chemolithoautotrophic, sulfate-reducing bacterium isolated from a Mid-Atlantic Ridge hydrothermal vent. Int J Syst Evol Microbiol 60:33–38 [View Article][PubMed]
    [Google Scholar]
  3. Anderson I., Saunders E., Lapidus A., Nolan M., Lucas S., Tice H., Del Rio T. G., Cheng J. F., Han C. et al. 2012; Complete genome sequence of the thermophilic sulfate-reducing ocean bacterium Thermodesulfatator indicus type strain (CIR29812T). Stand Genomic Sci 6:155–164 [View Article][PubMed]
    [Google Scholar]
  4. Auch A. F., Klenk H. P., Göker M. 2010a; Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2:142–148 [View Article]
    [Google Scholar]
  5. Auch A. F., von Jan M., Klenk H. P., Göker M. 2010b; Digital DNA–DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2:117–134 [View Article]
    [Google Scholar]
  6. Cord-Ruwisch R. 1985; A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Meth 4:33–36 [View Article]
    [Google Scholar]
  7. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. 2007; DNA–DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57:81–91 [View Article][PubMed]
    [Google Scholar]
  8. Heidelberg J. F., Seshadri R., Haveman S. A., Hemme C. L., Paulsen I. T., Kolonay J. F., Eisen J. A., Ward N., Methe B. et al. 2004; The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough. Nat Biotechnol 22:554–559 [View Article][PubMed]
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  10. Luo R., Liu B., Xie Y., Li Z., Huang W., Yuan J., He G., Chen Y., Pan Q. et al. 2012; SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 1:18 [View Article][PubMed]
    [Google Scholar]
  11. Meier-Kolthoff J. P., Auch A. F., Klenk H. P., Göker M. 2013; Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14:60 [View Article][PubMed]
    [Google Scholar]
  12. Moussard H., L'Haridon S., Tindall B. J., Banta A., Schumann P., Stackebrandt E., Reysenbach A. L., Jeanthon C. 2004; Thermodesulfatator indicus gen. nov., sp. nov., a novel thermophilic chemolithoautotrophic sulfate-reducing bacterium isolated from the Central Indian Ridge. Int J Syst Evol Microbiol 54:227–233 [View Article][PubMed]
    [Google Scholar]
  13. Richter M., Rosselló-Móra R. 2009; Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  15. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Tecnical Note 101 Newark, DE: MIDI;
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  17. Wayne L. G., Stackebrandt E., Kandler O., Colwell R. R., Krichevsky M. I., Truper H. G., Murray R. G. E., Moore W. E. C., Grimont P. A. D. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
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