1887

Abstract

A Gram-stain-negative, spore-forming, anaerobic bacterium designated strain RU4 was isolated from a saprolite core collected from Oak Ridge, Tennessee, USA. Cells were slightly curved rods and exhibited an outer membrane exterior to a thin cell wall. Strain RU4 formed heat-resistant endospores in late-log phase and stationary phase cultures. Under anaerobic conditions, strain RU4 grew by fermenting fumarate and maleate, but did not grow on glucose, glycerol, pyruvate, lactate, succinate, citrate, formate, acetate, propionate, butyrate or valerate. Strain RU4 did not reduce sulfate or ferric iron. The main cellular fatty acids were C cyclo, C and C. The DNA G+C content was 52 mol%. Analysis of the 16S rRNA, , , , and gene sequences indicated that the isolate is related to members of the family . Based on 92 % sequence similarity of the 16S rRNA gene to its closest relatives in the family and divergent physiological traits, the newly-cultivated isolate was assigned to a novel species of a new genus, gen. nov., sp. nov. The type strain of is RU4 (=DSM 29728=ATCC BAA-2723).

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2016-10-01
2022-01-24
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