1887

Abstract

A Gram-stain-negative, spore-forming, anaerobic bacterium designated strain RU4 was isolated from a saprolite core collected from Oak Ridge, Tennessee, USA. Cells were slightly curved rods and exhibited an outer membrane exterior to a thin cell wall. Strain RU4 formed heat-resistant endospores in late-log phase and stationary phase cultures. Under anaerobic conditions, strain RU4 grew by fermenting fumarate and maleate, but did not grow on glucose, glycerol, pyruvate, lactate, succinate, citrate, formate, acetate, propionate, butyrate or valerate. Strain RU4 did not reduce sulfate or ferric iron. The main cellular fatty acids were C17 : 0 cyclo, C16 : 0 and C15 : 0. The DNA G+C content was 52 mol%. Analysis of the 16S rRNA, rpoB, recA, infB, gyrB and atpD gene sequences indicated that the isolate is related to members of the family Sporomusaceae . Based on 92 % sequence similarity of the 16S rRNA gene to its closest relatives in the family Sporomusaceae and divergent physiological traits, the newly-cultivated isolate was assigned to a novel species of a new genus, Anaerosporomusa subterranea gen. nov., sp. nov. The type strain of Anaerosporomusa subterranea is RU4 (=DSM 29728=ATCC BAA-2723).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001275
2016-10-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/3848.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001275&mimeType=html&fmt=ahah

References

  1. Aziz R. K., Bartels D., Best A. A., DeJongh M., Disz T., Edwards R. A., Formsma K., Gerdes S., Glass E. M. et al.( 2008;). The RAST Server: rapid annotations using subsystems technology. . BMC Genomics 9: 75. [CrossRef] [PubMed]
    [Google Scholar]
  2. Biebl H., Schwab-Hanisch H., Spröer C., Lünsdorf H..( 2000;). Propionispora vibrioides, nov. gen., nov. sp., a new gram-negative, spore-forming anaerobe that ferments sugar alcohols. . Arch Microbiol 174: 239–247. [CrossRef] [PubMed]
    [Google Scholar]
  3. Boga H. I., Ludwig W., Brune A..( 2003;). Sporomusa aerivorans sp. nov., an oxygen-reducing homoacetogenic bacterium from the gut of a soil-feeding termite. . Int J Syst Evol Microbiol 53: 1397–1404. [CrossRef] [PubMed]
    [Google Scholar]
  4. Campbell C., Adeolu M., Gupta R. S..( 2015;). Genome-based taxonomic framework for the class Negativicutes: division of the class Negativicutes into the orders Selenomonadales emend., Acidaminococcales ord. nov. and Veillonellales ord. nov. . Int J Syst Evol Microbiol 65: 3203–3215. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M..( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81: 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  6. Church D. L., Simmon K. E., Sporina J., Lloyd T., Gregson D. B..( 2011;). Identification by 16S rRNA gene sequencing of Negativicoccus succinicivorans recovered from the blood of a patient with hemochromatosis and pancreatitis. . J Clin Microbiol 49: 3082–3084. [CrossRef] [PubMed]
    [Google Scholar]
  7. Cline J. D..( 1969;). Spectrophotometric determination of hydrogen sulfide in natural waters. . Limnol Oceanogr 14: 454–458.[CrossRef]
    [Google Scholar]
  8. Cole J. R., Wang Q., Fish J. A., Chai B., McGarrell D. M., Sun Y., Brown C. T., Porras-Alfaro A., Kuske C. R., Tiedje J. M..( 2014;). Ribosomal database project: data and tools for high throughput rRNA analysis. . Nucleic Acids Res 42: D633–D642. [CrossRef] [PubMed]
    [Google Scholar]
  9. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R..( 1994;). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  10. Gihring T. M., Zhang G., Brandt C. C., Brooks S. C., Campbell J. H., Carroll S., Criddle C. S., Green S. J., Jardine P. et al.( 2011;). A limited microbial consortium is responsible for extended bioreduction of uranium in a contaminated aquifer. . Appl Environ Microbiol 77: 5955–5965. [CrossRef] [PubMed]
    [Google Scholar]
  11. Jumas-Bilak E., Carlier J. P., Jean-Pierre H., Teyssier C., Gay B., Campos J., Marchandin H..( 2004;). Veillonella montpellierensis sp. nov., a novel, anaerobic, Gram-negative coccus isolated from human clinical samples. . Int J Syst Evol Microbiol 54: 1311–1316. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kane M. D., Breznak J. A..( 1991;). Acetonema longum gen. nov. sp. nov., an H2/CO2 acetogenic bacterium from the termite, Pterotermes occidentis. . Arch Microbiol 156: 91–98. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E..( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. . Int J Syst Bacteriol 38: 358–361. [CrossRef]
    [Google Scholar]
  15. Marchandin H., Teyssier C., Campos J., Jean-Pierre H., Roger F., Gay B., Carlier J. P., Jumas-Bilak E..( 2010;). Negativicoccus succinicivorans gen. nov., sp. nov., isolated from human clinical samples, emended description of the family Veillonellaceae and description of Negativicutes classis nov., Selenomonadales ord. nov. and Acidaminococcaceae fam. nov. in the bacterial phylum Firmicutes. . Int J Syst Evol Microbiol 60: 1271–1279. [CrossRef] [PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B..( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high- performance liquid chromatography. . Int J Syst Bacteriol 39: 159–167. [CrossRef]
    [Google Scholar]
  17. Miller L. T..( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16: 584–586.[PubMed]
    [Google Scholar]
  18. Moe W. M., Stebbing R. E., Rao J. U., Bowman K. S., Nobre M. F., da Costa M. S., Rainey F. A..( 2012;). Pelosinus defluvii sp. nov., isolated from chlorinated solvent-contaminated groundwater, emended description of the genus Pelosinus and transfer of Sporotalea propionica to Pelosinus propionicus comb. nov. . Int J Syst Evol Microbiol 62: 1369–1376. [CrossRef] [PubMed]
    [Google Scholar]
  19. Möller B., Oßmer R., Howard B. H., Gottschalk G., Hippe H..( 1984;). Sporomusa, a new genus of gram-negative anaerobic bacteria including Sporomusa sphaeroides spec. nov. and Sporomusa ovata spec. nov. . Arch Microbiol 139: 388–396. [CrossRef]
    [Google Scholar]
  20. Moriya Y., Itoh M., Okuda S., Yoshizawa A. C., Kanehisa M..( 2007;). KAAS: an automatic genome annotation and pathway reconstruction server. . Nucleic Acids Res 35: W182–W185. [CrossRef] [PubMed]
    [Google Scholar]
  21. Saldanha A. J..( 2004;). Java Treeview – extensible visualization of microarray data. . Bioinformatics 20: 3246–3248. [CrossRef] [PubMed]
    [Google Scholar]
  22. Sass H., Overmann J., Rütters H., Babenzien H. D., Cypionka H..( 2004;). Desulfosporomusa polytropa gen. nov., sp. nov., a novel sulfate-reducing bacterium from sediments of an oligotrophic lake. . Arch Microbiol 182: 204–211. [CrossRef] [PubMed]
    [Google Scholar]
  23. Sattley W. M., Jung D. O., Madigan M. T..( 2008;). Psychrosinus fermentans gen. nov., sp. nov., a lactate-fermenting bacterium from near-freezing oxycline waters of a meromictic Antarctic lake. . FEMS Microbiol Lett 287: 121–127. [CrossRef] [PubMed]
    [Google Scholar]
  24. Shah M., Lin C.-C., Kukkadapu R., Engelhard M. H., Zhao X., Wang Y., Barkay T., Yee N..( 2014;). Syntrophic effects in a subsurface clostridial consortium on Fe(III)-(oxyhydr)oxide reduction and secondary mineralization. . Geomicrobiol J 31: 101–115. [CrossRef]
    [Google Scholar]
  25. Shelobolina E. S., Nevin K. P., Blakeney-Hayward J. D., Johnsen C. V., Plaia T. W., Krader P., Woodard T., Holmes D. E., Vanpraagh C. G., Lovley D. R..( 2007;). Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses. . Int J Syst Evol Microbiol 57: 126–135. [CrossRef] [PubMed]
    [Google Scholar]
  26. Strömpl C., Tindall B. J., Lünsdorf H., Wong T. Y., Moore E. R., Hippe H..( 2000;). Reclassification of Clostridium quercicolum as Dendrosporobacter quercicolus gen. nov., comb. nov. . Int J Syst Evol Microbiol 50: 101–106. [CrossRef] [PubMed]
    [Google Scholar]
  27. Tamaoka J., Komagata K..( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25: 125–128. [CrossRef]
    [Google Scholar]
  28. Tamura K., Dudley J., Nei M., Kumar S..( 2007;). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24: 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  29. Teeling H., Waldmann J., Lombardot T., Bauer M., Glöckner F. O..( 2004;). TETRA: a web-service and a stand-alone program for the analysis and comparison of tetranucleotide usage patterns in DNA sequences. . BMC Bioinformatics 5: 163. [CrossRef] [PubMed]
    [Google Scholar]
  30. Ueki A., Watanabe M., Ohtaki Y., Kaku N., Ueki K..( 2014;). Description of Propionispira arcuata sp. nov., isolated from a methanogenic reactor of cattle waste, reclassification of Zymophilus raffinosivorans and Zymophilus paucivorans as Propionispira raffinosivorans comb. nov. and Propionispira paucivorans comb. nov. and emended description of the genus Propionispira. . Int J Syst Evol Microbiol 64: 3571–3577. [CrossRef] [PubMed]
    [Google Scholar]
  31. Viollier E., Inglett P. W., Hunter K., Roychoudhury A. N., Van Cappellen P..( 2000;). The ferrozine method revisited: Fe(II)/Fe(III) determination in natural waters. . Appl Geochem 15: 785–790. [CrossRef]
    [Google Scholar]
  32. Wang C. H., Kan L. P., Sun J. R., Yu C. M., Yin T., Huang T. W., Tsai W. C., Yang Y. S..( 2015;). Empyema caused by Anaeroglobus geminates, a case report with literature review. . Infection 43: 117–120. [CrossRef] [PubMed]
    [Google Scholar]
  33. Wolin E. A., Wolin M. J., Wolfe R. S..( 1963;). Formation of methane by bacterial extracts. . J Biol Chem 238: 2882–2886.
    [Google Scholar]
  34. Yarza P., Yilmaz P., Pruesse E., Glöckner F. O., Ludwig W., Schleifer K. H., Whitman W. B., Euzéby J., Amann R., Rosselló-Móra R..( 2014;). Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. . Nat Rev Microbiol 12: 635–645. [CrossRef] [PubMed]
    [Google Scholar]
  35. Yutin N., Galperin M. Y..( 2013;). A genomic update on clostridial phylogeny: gram-negative spore formers and other misplaced clostridia. . Environ Microbiol 15: 2631–2641. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001275
Loading
/content/journal/ijsem/10.1099/ijsem.0.001275
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error