1887

Abstract

A Gram-stain-positive coccus, strain JC304, was isolated from a saltern of Nari along the Bhavnagar Coast, Gujarat, India. The 16S rRNA gene sequence analysis and sequence comparison data indicated that JC304 represented a member of the genus Salinicoccus and was most closely related to Salinicoccus roseus 9 (99.6 %), Salinicoccus luteus YIM 70202 (97.0 %), Salinicoccus hispanicus J-82 (97.0 %) and the remaining species of the genus Salinicoccus (<97 %). Genome relatedness based on DNA–DNA hybridization of JC304 with the type strains of the most closely related species was less than 46 % and the ΔTm was >5 °C indicating that the strain represents a novel species of the genus Salinicoccus . Independent and concatenated phylogenetic analysis of recA/fusA gene translated product showed a clear distinction of JC304 from its phylogenetic neighbors. Diphosphotidylglycerol, phosphatidylglycerol, an unidentified glycolipid and three unidentified lipids (L1, L2 and L3) were the polar lipids of JC304. Iso-C15 : 0 and anteiso-C15 : 0 were the major (>10 %) fatty acids in strain JC304. The cell-wall amino acids were l-lysine and d-glycine. Hopanoids were not detected. The major isoprenoid quinone was menaquinone (MK-6). The DNA G+C content of JC304 was 48 mol%. On the basis of physiological, genotypic, phylogenetic and chemotaxonomic analyses, strain JC304 is considered to represent a novel species of the genus Salinicoccus , for which the name Salinicoccus amylolyticus sp. nov. is proposed. The type strain is JC304 (=KCTC 33661=LMG 28757).

Keyword(s): new species and Salinicoccus
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2016-10-01
2019-10-19
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