1887

Abstract

Three closely related, non-sporulating, aerobic, Gram-stain-negative, motile, rod-shaped isolates (S5-53, S6-62 and S6-64) were obtained from mucus of corals Favia veroni from the Andaman Sea, India. Colonies grown on marine agar were small, circular and cream-coloured. Heterotrophic growth was observed at 10–40 °C and pH 6–10; optimum growth occurred at 25–30 °C and pH 7–8. 16S rRNA gene sequence analysis confirmed the isolates belonged to the genus Sulfitobacter and the three isolates shared more than 99 % pairwise sequence similarity. Strain S5-53 shared highest 16S rRNA gene sequence similarity of 98.43 % with Sulfitobacter dubius KMM 3554. DNA–DNA relatedness among the three isolates was above 70 % whereas strain S5-53 showed less than 70 % relatedness with the type strains of closely related species. The DNA G+C content of strain S5-53 was 61 mol%. It contained phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and diphosphatidylglycerol as major polar lipids. Predominant fatty acids included C18 : 1 ω7c, C18 : 1 ω7c 11-methyl, C16 : 0 and C10 : 0 3-OH. Q10 was the major respiratory quinone. Based on this polyphasic analysis, the new isolates (S5-53, S6-62 and S6-64) are considered to represent a novel species of the genus Sulfitobacter , for which the name Sulfitobacter faviae sp. nov. is proposed. The type strain is S5-53(=JCM 31093=LMG 29156).

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2016-10-01
2019-10-18
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