1887

Abstract

Three Gram-stain-negative, rod-shaped bacteria, designated strains NH153, F-2-11 and M-1-78, were isolated from surface seawater of the South China Sea and the East China Sea. The three isolates were able to grow at 15–45 °C (optimum 28–37 °C), but no growth occurred at 4 or 50 °C. The pH range for growth was pH 5.5–9.5 (optimum pH 7.5–8.5). The isolates required sea salts for growth and growth occurred in the presence of 0–10 % (w/v) NaCl (optimum 3–5 %); no growth occurred in the presence of 12.0, 15.0 or 20.0 % (w/v) NaCl. They were positive for hydrolysis of gelatin and Tween 80. The sole respiratory quinone was ubiquinone-8 (Q-8). The major cellular fatty acids (>10 %) were C, C 7 and summed feature 3 (C 7 and/or iso-C 2-OH). The major polar lipid components were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid, one unidentified phospholipid and one unidentified lipid. The genomic DNA G+C content of strain NH153 was 41.4 mol%. Based on 16S rRNA gene sequence analysis, the isolates were closely related to the type strain of (98.0–98.6 % sequence similarity). The 16S rRNA gene sequence similarities between the three isolates were 98.8–99.7 %. Phylogenetic analysis indicated that they formed a distinct lineage and clustered with and . The level of DNA–DNA relatedness among the three isolates was 78.0–85.5 %. Strain NH153 exhibited average nucleotide identity values of 93.4 and 84.2 % with respect to JCM 18891 and JCM 17292, respectively. The genome-to-genome distance analysis revealed that strain NH153 shared 52.4 % DNA relatedness with JCM 18891 and 28.1 % with JCM 17292. On the basis of the phenotypic, genotypic and chemotaxonomic characterizations, as well as phylogenetic inference obtained in this study, strains NH153, F-2-11 and M-1-78 represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is NH153 (=CGMCC 1.15370=DSM 100951), and F-2-11 (=CGMCC 1.15364=DSM 100953) and M-1-78 (=CGMCC 1.15365=DSM 100952), are additional strains of the species.

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2016-09-01
2020-01-28
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