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Abstract

The taxonomy of strain 1DS3-10, a Gram-staining-positive, endospore-forming bacterium isolated from rice rhizosphere, was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that the novel strain was grouped with established members of the genus and appeared to be closely related to the type strains DSM 5391 (97.9 %), DSM 11 (97.7 %), JCM 21709 (97.3 %), JCM 21710 (97.3 %), CGMCC 1.10115 (97.3 %) and FO-92 (97.1 %). The fatty acid profile of strain 1DS3-10, which showed a predominance of iso-C and anteiso-C, supported the allocation of the strain to the genus . The predominant menaquinone was MK-7 (100 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unknown aminolipids. Cell-wall peptidoglycan contained -diaminopimelic acid. DNA–DNA hybridization values between strain 1DS3-10 and the type strains of closely related species were 25–33 %, which supported that 1DS3-10 represented a novel species in the genus . The results of some physiological and biochemical tests also allowed the phenotypic differentiation of strain 1DS3-10 from the most closely related recognized species. On the basis of the phylogenetic and phenotypic evidence, strain 1DS3-10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the novel species is 1DS3-10 (=ACCC 19781=DSM 29761).

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2016-09-01
2021-07-26
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