1887

Abstract

A novel Gram-stain-negative, motile, aerobic and rod-shaped alphaproteobacterium, designated strain KHS03, was isolated from the intestinal tract of a Japanese flying squid, , which was collected from the East Sea, Korea. The new isolate shared 97.4 % 16S rRNA gene sequence similarity with NH52F. The isolate grew optimally at 25 °C and pH 7 in the presence of 1–2 % (w/v) NaCl, and had an absolute requirement of sodium ions for growth. The major cellular fatty acid was C 7. The primary isoprenoid quinone was ubiquinone-10. Polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and two unidentified lipids. Genotypic analyses indicated that the whole genomic DNA of the isolate had a G+C content of 59.9 mol%. DNA–DNA hybridization showed that the isolate shared 17.1±2.3 % (reciprocal 17.0±1.9 %) genomic relatedness with the closest related type strain, NH52F. Strain KHS03 is thus suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KHS03 (=KCTC 42412=JCM 31160).

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2016-09-01
2020-04-09
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