1887

Abstract

Two bacterial strains, HKU54 and HKU55, were isolated from the oral cavity of two Chinese cobras (Naja atra) in Hong Kong. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU54 and HKU55, and the two strains shared 99.0 % sequence identities with T sukamurella inchonensis ATCC 700082. The two strains had unique biochemical profiles distinguishable from closely related species of the genus Tsukamurella . DNA–DNA hybridization confirmed that they belonged to the same species (≥92.1±7.9 % DNA–DNA relatedness) but were distinct from all other known species of the genus Tsukamurella (≤52.6±5.3 % DNA–DNA relatedness). Chemotaxonomic and morphological analyses of the two strains also demonstrated results consistent with their classification in the genus Tsukamurella . The DNA G+C contents of strains HKU54 and HKU55 were 69.2±1.5 mol% and 69.2±1.3 mol% (mean±sd; n=3) respectively. A novel species, Tsukamurella serpentis sp. nov., is proposed to accommodate strains HKU54 and HKU55, with HKU54 (=JCM 31017=DSM 100915) designated as the type strain.

Keyword(s): novel , serpentis , snake and Tsukamurella
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2016-09-01
2019-10-14
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