1887

Abstract

Two bacterial strains, HKU54 and HKU55, were isolated from the oral cavity of two Chinese cobras () in Hong Kong. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU54 and HKU55, and the two strains shared 99.0 % sequence identities with ATCC 700082. The two strains had unique biochemical profiles distinguishable from closely related species of the genus . DNA–DNA hybridization confirmed that they belonged to the same species (≥92.1±7.9 % DNA–DNA relatedness) but were distinct from all other known species of the genus (≤52.6±5.3 % DNA–DNA relatedness). Chemotaxonomic and morphological analyses of the two strains also demonstrated results consistent with their classification in the genus . The DNA G+C contents of strains HKU54 and HKU55 were 69.2±1.5 mol% and 69.2±1.3 mol% (mean±; =3) respectively. A novel species, sp. nov., is proposed to accommodate strains HKU54 and HKU55, with HKU54 (=JCM 31017=DSM 100915) designated as the type strain.

Keyword(s): novel , serpentis , snake and Tsukamurella
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001187
2016-09-01
2020-04-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/9/3329.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001187&mimeType=html&fmt=ahah

References

  1. Brown B. A., Springer B., Steingrube V. A., Wilson R. W., Pfyffer G. E., Garcia M. J., Menendez M. C., Rodriguez-Salgado B., Jost K. C. et al. 1999; Description of Mycobacterium wolinskyi sp. nov. and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy. Int J Syst Bacteriol49:1493–1511 [CrossRef][PubMed]
    [Google Scholar]
  2. Collins M. D., Smida J., Dorsch M., Stackebrandt E.. 1988; Tsukamurella gen. nov. Harboring Corynebacterium paurometabolum and Rhodococcus aurantiacus. Int J Syst Bacteriol38:385–391 [CrossRef]
    [Google Scholar]
  3. Conville P. S., Witebsky F. G.. 1998; Variables affecting results of sodium chloride tolerance test for identification of rapidly growing mycobacteria. J Clin Microbiol36:1555–1559[PubMed]
    [Google Scholar]
  4. Goodfellow M., Kumar Y.. 2012; Genus I. Tsukamurella Collins, Smida, Dorsch and Stackebrandt. 1988, 387. In Bergey’s Manual of Systematic Bacteriology, 2nd edn.vol. 5 . The Actinobacteria, part A and B , pp.500–509 Edited by Goodfellow M., Kämpfer P., Busse B. H.-J., Trujillo M. E., Suzuki K.-I., Ludwig W., Whitman W. B.. New York: Springer;[CrossRef]
    [Google Scholar]
  5. Hall T. A.. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser41:95–98
    [Google Scholar]
  6. Jiang Y., Chen X., Han L., Huang X. S., Qiu S. M., Jiang. C. L.. 2013; Diversity of cultivable actinomycetes in 6 species of herbivore feces. Int J Microbiol Res1:76–84
    [Google Scholar]
  7. Kattar M. M., Cookson B. T., Carlson L. D., Stiglich S. K., Schwartz M. A., Nguyen T. T., Daza R., Wallis C. K., Yarfitz S. L. et al. 2001; Tsukamurella strandjordae sp. nov., a proposed new species causing sepsis. J Clin Microbiol39:1467–1476 [CrossRef][PubMed]
    [Google Scholar]
  8. Lechevalier M. P., De Bievre C., Lechevalier H.. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol5:249–260 [CrossRef]
    [Google Scholar]
  9. Maeda Y., Stanley T., Stirling J., Griffiths M., Calvert A., Stuart Elborn J., Cherie Millar B., Goldsmith C. E., Rendall J. et al. 2010; No evidence of transmission of bacteria between reptiles and a CF patient–a case report of a young adult CF patient and reptiles. Zoonoses Public Health57:e47e53 [CrossRef][PubMed]
    [Google Scholar]
  10. Marmur J., Doty P.. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  11. Nam S. W., Chun J., Kim S., Kim W., Zakrzewska-Czerwinska J., Goodfellow M.. 2003; Tsukamurella spumae sp. nov., a novel actinomycete associated with foaming in activated sludge plants. Syst Appl Microbiol26:367–375 [CrossRef][PubMed]
    [Google Scholar]
  12. Nam S. W., Kim W., Chun J., Goodfellow M.. 2004; Tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol54:1209–1212 [CrossRef][PubMed]
    [Google Scholar]
  13. Olson J. B., Harmody D. K., Bej A. K., McCarthy P. J.. 2007; Tsukamurella spongiae sp. nov., a novel actinomycete isolated from a deep-water marine sponge. Int J Syst Evol Microbiol57:1478–1481 [CrossRef][PubMed]
    [Google Scholar]
  14. Park S. W., Kim S. M., Park S. T., Kim Y. M.. 2009; Tsukamurella carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete. Int J Syst Evol Microbiol59:1541–1544 [CrossRef][PubMed]
    [Google Scholar]
  15. Rhuland L. E., Work E., Denman R. F., Hoare D. S.. 1955; The Behavior of the Isomers of α,ε-Diaminopimelic Acid on Paper Chromatograms. J Am Chem Soc77:4844–4846 [CrossRef]
    [Google Scholar]
  16. Sasser M.. 1990; Identification of bacteria through fatty acid analysis. In Methods in Phytobacteriology , pp.199–204 Edited by Klement Z., Rudolph K., Sands D. C.. Budapest: Akademiai Kiado;
    [Google Scholar]
  17. Seong C. N., Kim Y. S., Baik K. S., Choi S. K., Kim M. B., Kim S. B., Goodfellow M.. 2003; Tsukamurella sunchonensis sp. nov., a bacterium associated with foam in activated sludge. J Microbiol41:83–88
    [Google Scholar]
  18. Shek K. C., Tsui K. L., Lam K. K., Crow P., Ng K. H., Ades G., Yip K. T., Grioni A., Tan K. S. et al. 2009; Oral bacterial flora of the Chinese cobra (Naja atra) and bamboo pit viper (Trimeresurus albolabris) in Hong Kong SAR, China. Hong Kong Med J15:183–190[PubMed]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R.. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology , pp.607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Staneck J. L., Roberts G. D.. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28:226–231[PubMed]
    [Google Scholar]
  21. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  22. Teng J. L., Tang Y. Y., Wong S. S., Ngan A. H., Huang Y., Tsang C. C., Choi G. K., Lau S. K., Woo P. C.. 2015; Tsukamurella hongkongensis sp. nov. and Tsukamurella sinensis sp. nov., isolated from patients with keratitis, catheter-related bacteraemia and conjunctivitis in Hong Kong. Int J Syst Bacteriol66:391–397[CrossRef]
    [Google Scholar]
  23. Tindall B. J.. 1990; Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Let66:199–202 [CrossRef]
    [Google Scholar]
  24. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R.. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. , pp.330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. To K. K., Fung A. M., Teng J. L., Curreem S. O., Lee K. C., Yuen K. Y., Lam C. W., Lau S. K., Woo P. C.. 2013; Characterization of a Tsukamurella pseudo-outbreak by phenotypic tests, 16S rRNA sequencing, pulsed-field gel electrophoresis, and metabolic footprinting. J Clin Microbiol51:334–338 [CrossRef][PubMed]
    [Google Scholar]
  26. Weon H.-Y., Yoo S.-H., Anandham R., Schumann P., Kroppenstedt R. M., Kwon S.-W., Stackebrandt E.. 2010; Tsukamurella soli sp. nov., isolated from soil. In J Sys Bacteriol60:1667–1671 [CrossRef]
    [Google Scholar]
  27. Woo P. C., Fong A. H., Ngan A. H., Tam D. M., Teng J. L., Lau S. K., Yuen K. Y.. 2009; First report of Tsukamurella keratitis: association between T . tyrosinosolvens and T. pulmonis and ophthalmologic infections. J Clin Microbiol47:1953–1956 [CrossRef][PubMed]
    [Google Scholar]
  28. Woo P. C., Ngan A. H., Lau S. K., Yuen K. Y.. 2003; Tsukamurella conjunctivitis: a novel clinical syndrome. J Clin Microbiol41:3368–3371 [CrossRef][PubMed]
    [Google Scholar]
  29. Yassin A. F., Rainey F. A., Brzezinka H., Burghardt J., Lee H. J., Schaal K. P.. 1995; Tsukamurella inchonensis sp. nov. Int J Syst Bacteriol45:522–527 [CrossRef][PubMed]
    [Google Scholar]
  30. Yassin A. F., Rainey F. A., Brzezinka H., Burghardt J., Rifai M., Seifert P., Feldmann K., Schaal K. P.. 1996; Tsukamurella pulmonis sp. nov. Int J Syst Bacteriol46:429–436 [CrossRef][PubMed]
    [Google Scholar]
  31. Yassin A. F., Rainey F. A., Burghardt J., Brzezinka H., Schmitt S., Seifert P., Zimmermann O., Mauch H., Gierth D. et al. 1997; Tsukamurella tyrosinosolvens sp. nov. Int J Syst Bacteriol47:607–614 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001187
Loading
/content/journal/ijsem/10.1099/ijsem.0.001187
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error