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Abstract

An agarose- and alginate-assimilating, Gram-reaction-negative, non-motile, rod-shaped bacterium, designated strain SA2, was isolated from the gut of a turban shell sea snail (Turbo cornutus) collected near Noto Peninsula, Ishikawa Prefecture, Japan. The 16S rRNA gene sequence of strain SA2 was 99.59 % identical to that of Vibrio rumoiensis DSM 19141 and 98.19 % identical to that of Vibrio litoralis DSM 17657. This suggested that strain SA2 could be a subspecies of V. rumoiensis or V. litoralis . However, DNA–DNA hybridization results showed only 37.5 % relatedness to DSM 19141 and 44.7 % relatedness to DSM 17657, which was far lower than the 70 % widely accepted to define common species. Strain SA2 could assimilate agarose as a sole carbon source, whereas strains DSM 19141 and DSM 17657 could not assimilate it at all. Furthermore, results using API 20NE and API ZYM kits indicated that their enzymic and physiological phenotypes were also different. These results suggested that strain SA2 represented a novel species within the genus Vibrio . The major isoprenoid quinone in SA2 was Q-8, and its major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major fatty acids were summed feature 3, (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, and summed feature 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c). The DNA G+C content of SA2 was 40.7 mol%. The name proposed for this novel species of the genus Vibrio is Vibrio algivorus sp. nov., with the type strain designated SA2 (=DSM 29824=NBRC 111146).

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2016-08-01
2019-10-17
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