1887

Abstract

A Gram-stain-negative, rod-shaped, yellow-pigmented, gliding bacterial strain, designated S5-23-3, was isolated from a sediment sample of the Yellow Sea in China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S5-23-3 was related to the genus and had highest 16S rRNA gene sequence similarities with JCM 15527 (97.6 %), CECT 7392 (97.4 %) and KCTC 32261(97.2 %). The predominant cellular fatty acids were iso-C, iso-C G, iso-C 3-OH and iso-C 3-OH. Strain S5-23-3 contained MK-6 as the predominant menaquinone. The polar lipid profile contained phosphatidylethanolamine, two aminolipids, one aminoglycolipid, one aminophospholipid, one unidentified phospholipid and seven unidentified polar lipids. The genomic DNA G+C content of strain S5-23-3 was 36.1 mol%. Combined data from phenotypic, chemotaxonomic, phylogenetic and DNA–DNA relatedness studies demonstrated that strain S5-23-3 is a representative of a novel species of the genus , for which the name sp. nov. (type strain S5-23-3=LMG 28075=DSM 28134) is proposed.

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2016-08-01
2024-10-09
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References

  1. Alarico S., Rainey F. A., Empadinhas N., Schumann P., Nobre M. F., da Costa M. S. 2002; Rubritepida flocculans gen. nov., sp. nov., a new slightly thermophilic member of the alpha-1 subclass of the Proteobacteria . Syst Appl Microbiol 25:198–206 [View Article][PubMed]
    [Google Scholar]
  2. Begum Z., Srinivas T. N., Manasa P., Sailaja B., Sunil B., Prasad S., Shivaji S. 2013; Winogradskyella psychrotolerans sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from Arctic sediment. Int J Syst Evol Microbiol 63:1646–1652 [View Article][PubMed]
    [Google Scholar]
  3. Bernardet J.-F., Bowman J. P. 2006; The genus Flavobacterium . In The Prokaryotes, 3rd edn. vol. 7 pp 481–531 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; [CrossRef]
    [Google Scholar]
  4. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [View Article][PubMed]
    [Google Scholar]
  5. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  7. Huss V. A., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
    [Google Scholar]
  8. Kang C. H., Lee S. Y., Yoon J. H. 2013; Winogradskyella litorisediminis sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 63:1793–1799 [View Article][PubMed]
    [Google Scholar]
  9. Kim J. Y., Park S. H., Seo G. Y., Kim Y. J., Oh D. C. 2015; Winogradskyella eckloniae sp. nov., a marine bacterium isolated from the brown alga Ecklonia cava . Int J Syst Evol Microbiol 65:2791–2796 [View Article][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Kim S. J., Choi Y. R., Park S. J., Kim J. G., Shin K. S., Roh D. H., Rhee S. K. 2013; Winogradskyella pulchriflava sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 63:3062–3068 [View Article][PubMed]
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  13. Lee D. H., Cho S. J., Kim S. M., Lee S. B. 2013; Winogradskyella damuponensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63:321–326 [View Article][PubMed]
    [Google Scholar]
  14. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  15. Margesin R., Gander S., Zacke G., Gounot A. M., Schinner F. 2003; Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. Extremophiles 7:451–458 [View Article][PubMed]
    [Google Scholar]
  16. Margesin R., Zhang D. C. 2013; Humitalea rosea gen. nov., sp. nov., an aerobic bacteriochlorophyll-containing bacterium of the family Acetobacteraceae isolated from soil. Int J Syst Evol Microbiol 63:1411–1416 [View Article][PubMed]
    [Google Scholar]
  17. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  18. Montero-Calasanz M. C., Göker M., Rohde M., Spröer C., Schumann P., Busse H. J., Schmid M., Tindall B. J., Klenk H. P., Camacho M. 2013; Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium . Int J Syst Evol Microbiol 63:4386–4395 [View Article][PubMed]
    [Google Scholar]
  19. Nedashkovskaya O. I., Kim S. B., Han S. K., Snauwaert C., Vancanneyt M., Swings J., Kim K. O., Lysenko A. M., Rohde M. et al. 2005; Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae . Int J Syst Evol Microbiol 55:49–55 [View Article][PubMed]
    [Google Scholar]
  20. Nedashkovskaya O. I., Kukhlevskiy A. D., Zhukova N. V. 2012; Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia . Int J Syst Evol Microbiol 62:1450–1456 [View Article][PubMed]
    [Google Scholar]
  21. Park S., Yoon J. H. 2013; Winogradskyella undariae sp. nov., a member of the family Flavobacteriaceae isolated from a brown algae reservoir. Antonie Van Leeuwenhoek 104:619–626 [View Article][PubMed]
    [Google Scholar]
  22. Park S., Park J. M., Won S. M., Bae K. S., Yoon J. H. 2014; Winogradskyella wandonensis sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 64:1520–1525 [View Article][PubMed]
    [Google Scholar]
  23. Park S., Park J. M., Won S. M., Yoon J. H. 2015; Winogradskyella crassostreae sp. nov., isolated from an oyster (Crassostrea gigas). Int J Syst Evol Microbiol 65:2890–2895 [View Article][PubMed]
    [Google Scholar]
  24. Pinhassi J., Nedashkovskaya O. I., Hagström A., Vancanneyt M. 2009; Winogradskyella rapida sp. nov., isolated from protein-enriched seawater. Int J Syst Evol Microbiol 59:2180–2184 [View Article][PubMed]
    [Google Scholar]
  25. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V. 2009; Winogradskyella arenosi sp. nov., a member of the family Flavobacteriaceae isolated from marine sediments from the Sea of Japan. Int J Syst Evol Microbiol 59:1443–1446 [View Article][PubMed]
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  27. Sambrook J., Frisch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. Technical Note 101 Newark, DE: MIDI;
    [Google Scholar]
  29. Süßmuth R., Eberspächer J., Haag R., Springer W. 1987 Biochemisch-Mikrobiologisches Praktikum Stuttgart: Georg Thieme Verlag;
    [Google Scholar]
  30. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  31. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  32. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiology Letters 66:199–202 [View Article]
    [Google Scholar]
  33. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp. 330–393 Edited by Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. , Schmidt T. M., Snyder L. R. Washington, DC: ASM Press;
    [Google Scholar]
  34. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the Ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  35. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English Translation of Microbiology (Beijing)] 16:176–178
    [Google Scholar]
  36. Zhang D. C., Wang H. X., Liu H. C., Dong X. Z., Zhou P. J. 2006; Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. Int J Syst Evol Microbiol 56:2921–2925 [View Article][PubMed]
    [Google Scholar]
  37. Zhang D. C., Redzic M., Schinner F., Margesin R. 2011; Glaciimonas immobilis gen. nov., sp. nov., a member of the family Oxalobacteraceae isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 61:2186–2190 [View Article][PubMed]
    [Google Scholar]
  38. Zhang D. C., Margesin R. 2014; Martelella radicis sp. nov. and Martelella mangrovi sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 64:3104–3108 [View Article][PubMed]
    [Google Scholar]
  39. Zhang D. C., Margesin R. 2015; Gelidibacter sediminis sp. nov., isolated from a sediment sample of the Yellow Sea. Int J Syst Evol Microbiol 65:2304–2309 [View Article][PubMed]
    [Google Scholar]
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