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Abstract

An extremely halophilic archaeal strain YJ-50-S2 was isolated from Yangjiang marine solar saltern, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-50-S2 was able to grow at 25–50 °C (optimum 37 °C), with 0.9–4.8 M NaCl (optimum 2.6 M NaCl) and 0–1.0 M MgCl (optimum 0.03 M MgCl), and at pH 5.0–9.5 (optimum pH 7.5). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 5 % (w/v). The 16S rRNA gene and ′ gene of strain YJ-50-S2 were phylogenetically related to the corresponding genes of GX10 (97.0 % and 90.5 % similarities, respectively). The major polar lipids of strainYJ-50-S2 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively. Several unidentified glycolipids were also detected. The DNA G+C content of strain YJ-50-S2 was 65.2 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ-50-S2represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YJ-50-S2 (=CGMCC 1.12572=JCM 30033).

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2016-08-01
2020-09-30
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