1887

Abstract

Two novel (S9 and S12) Gram-stain-positive, rod shaped, non-motile and endospore forming bacteria were isolated from Narayan Sarovar lake, in India. The high 16S rRNA gene sequence similarity (99.9 %) and DNA–DNA relatedness (86±2 %) indicated that strains S9and S12 were members of a single species. Based on the 16S rRNA gene sequence analysis, these strains were identified as belonging to the class and were most closely related to PN-105 (96.8 % sequence similarity), JSM 071004 (96.5 %) and JC167 (96.1 %). However, these strains shared only 90.3 % 16S rRNA gene sequence similarity with subsp. DSM 10, indicating that they might not be members of the genus . The cell-wall peptidoglycan contained -diaminopimelic acid. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and an unknown lipid. The predominant isoprenoid quinone was MK-7. Major fatty acids (>5 %) included anteiso-C, C, iso-C, anteiso-C, isoCand summed feature 3. The results of phylogenetic, chemotaxonomic and biochemical tests allowed a clear differentiation of strains S9 and S12 from all other members of the family The strains therefore represent a novel member of a new genus from the family , for which the name gen. nov., sp. nov. is proposed. The type strain is S9(=KCTC 33633=LMG 28644). Based on the present study, it is also proposed to transfer and to this new genus as comb. nov. and comb. nov.

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2016-07-01
2020-03-29
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