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Abstract

A novel Gram-stain-negative, aerobic, motile by gliding and filamentous strain, designated 772,was isolated from surface-sterilized root tissue of maize planted in the Fangshan District of Beijing, China. 16S rRNA gene sequence analysis indicated that strain 772 was closely related to Filimonas endophytica SR2-06 and Filimonas lacunae YT21of the family Chitinophagaceae with sequence similarities of 99.0 and 96.9 %, respectively. However, the new isolate exhibited relatively low levels of DNA–DNA relatedness with respect to Filimonas. endophytica KCTC 42060 (18.7±1.8 %) and Filimonas. lacunae DSM 21054(17.9±2.0%). The DNA G+C content of strain 772 was 44.9 mol%. The respiratory quinone was menaquinone-7 and the polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and one unidentified lipid. The major fatty acids were iso-C15 : 0 and iso-C15 : 1 G. The results of the physiological and biochemical tests and minor differences in the fatty acid profiles allowed the clear phenotypic differentiation of strain 772 from the closely related species Filimonas. endophytica andF. lacunae. Strain 772thus represents a novel species within the genus Filimonas , for which the name Filimonas zeae sp. nov. is proposed. The type strain is 772 (=CGMCC 1.15290=DSM 100760).

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2016-07-01
2019-10-22
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