1887

Abstract

A Gram-stain-negative, rod-shaped bacterium, designated Ar-125, was isolated from Antarctic seawater. It produced carotenoid-like pigments and did not produce Bchl a. Ar-125 was positive for hydrolysis of DNA, aesculin, gelatin, starch, Tween 40 and Tween 60. The sole respiratory quinone was MK-6. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid, one unidentified glycolipid and two unidentified lipids. The principal fatty acids were branched-chain fatty acids, including iso-C15 : 0, iso-C15 : 1 G, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C16 : 0, iso-C17 : 0 3-OH, iso-C16 : 0 3-OH and iso-C15 : 0 3-OH, as well as C15 : 0. The genomic DNA G+C content was 31.8 mol%. On the basis of 16S rRNA gene sequence analysis, Ar-125 is closely related to the species of the genera Bizionia (with 16S rRNA gene pairwise sequence similarity of 93.7–96.5 %), Formosa (94.3–95.8 %), Gaetbulibacter (94.2–95.7 %), Geojedonia (95.5 %), Gelidibacter (93.3–95.4 %), Meridianimaribacter (95.3 %) and Psychroserpens (94.8–95.3 %), of the family Flavobacteriaceae . Phylogenetic analysis indicated that it represented an independent lineage and that the closest relatives were members of the genus Gelidibacter . Differential phenotypic properties and chemotaxonomic differences, together with phylogenetic distinctiveness, revealed that Ar-125 could be differentiated from members of closely related genera. Therefore, it is proposed that Ar-125 represents a novel species in a new genus, for which the name Aquaticitalea lipolytica gen. nov., sp. nov. (type strain Ar-125 =CGMCC 1.15295=JCM 30876) is proposed.

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2016-07-01
2019-10-17
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