1887

Abstract

A Gram-stain-positive, catalase-positive, facultatively anaerobic, non-motile, coryneform bacterium, designated strain 32, was isolated from a closed pus sample from a patient having finger necrosis in Korea. Strain 32 was considered as representing a novel species according to its initial identification by matrix-assisted laser desorption/ionization-time-of-flight MS. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 32belonged to the genus Dermabacter and was closely related to Dermabacter hominis DSM 7083 (=ATCC 49369) (98.34 % similarity). Optimal growth was observed at 30–40 °C and pH 7. Growth occurred in the presence of 0–6 % (w/v) NaCl. Menaquinones MK-8, MK-7 and MK-9 were the major respiratory quinones. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, glycolipid and two unknown lipids. The major cellular fatty acids were anteiso-C17 : 0, anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0. The DNA G+C content of strain 32 was 62.58 mol%, and the mean level of DNA–DNA relatedness between strain 32and D. hominis ATCC 49369 was 49±1.6 %. Based on the phenotypic and genotypic characteristics, strain 32 is confirmed to represent a novel species of the genus Dermabacter , for which the name Dermabacter jinjuensis sp. nov. is proposed. The type strain is 32(=NCCP 16133=DSM 101003).

Keyword(s): Dermabacter and new taxa
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001092
2016-07-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/7/2573.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001092&mimeType=html&fmt=ahah

References

  1. Bavbek M., Caner H., Arslan H., Demirhan B., Tunçbilek S., Altinörs N..( 1998;). Cerebral Dermabacter hominis abscess. . Infection 26: 181–183. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W..( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57: 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E..( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100: 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E..( 1989;). Fluorometric Deoxyribonucleic acid-Deoxyribonucleic acid Hybridization in Microdilution wells as an alternative to membrane filter Hybridization in which Radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39: 224–229. [CrossRef]
    [Google Scholar]
  5. Felsenstein J..( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17: 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  6. Funke G., Stubbs S., Pfyffer G. E., Marchiani M., Collins M. D..( 1994;). Characteristics of CDC group 3 and group 5 coryneform bacteria isolated from clinical specimens and assignment to the genus Dermabacter. . J Clin Microbiol 32: 1223–1228.[PubMed]
    [Google Scholar]
  7. Gruner E., Steigerwalt A. G., Hollis D. G., Weyant R. S., Weaver R. E., Moss C. W., Daneshvar M., Brenner D. J..( 1994;). Recognition of Dermabacter hominis, formerly CDC fermentative coryneform group 3 and group 5, as a potential human pathogen. . J Clin Microbiol 32: 1918–1922.[PubMed]
    [Google Scholar]
  8. Gómez-Garcés J. L., Oteo J., García G., Aracil B., Alós J. I., Funke G..( 2001;). Bacteremia by Dermabacter hominis, a rare pathogen. . J Clin Microbiol 39: 2356–2357. [CrossRef] [PubMed]
    [Google Scholar]
  9. Hollis D. G..( 1992;). Potential new CDC coryneform groups. Presented at the 92nd General Meeting of the American Society for Microbiology, 26–30. New Orleans, LA, USA:: American Society for Microbiology;.
    [Google Scholar]
  10. Jones D., Collins M. D..( 1988;). Taxonomic studies on some human cutaneous coryneform bacteria: Description of Dermabacter hominis gen.nov., sp.nov. . FEMS Microbiology Letters 51: 51–55. [CrossRef]
    [Google Scholar]
  11. Kim S. R., Rhee M. S., Kim B. C., Kim K. H..( 2007;). Modeling the inactivation of Escherichia coli O157:H7 and generic Escherichia coli by supercritical carbon dioxide. . Int J Food Microbiol 118: 52–61. [CrossRef] [PubMed]
    [Google Scholar]
  12. Lane D. J..( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt. E., Goodfellow M.. Chichester:: Wiley;.
    [Google Scholar]
  13. Lee H.-J., Cho C.-H., Kwon M.-J., Nam M.-H., Lee K.-N., Lee C.-K..( 2011;). A patient with fatal septicemia caused by a rare pathogen dermabacter hominis. . Infection and Chemotherapy 43: 86–88. [CrossRef]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B..( 1989;). Precise measurement of the G+C content of Deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriopl 39: 159–167. [CrossRef]
    [Google Scholar]
  15. Minnikin D. E., Collins M. D., Goodfellow M..( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47: 87–95. [CrossRef]
    [Google Scholar]
  16. Radtke A., Bergh K., Øien C. M., Bevanger L. S..( 2001;). Peritoneal dialysis-associated peritonitis caused by Dermabacter hominis. . J Clin Microbiol 39: 3420–3421. [CrossRef] [PubMed]
    [Google Scholar]
  17. Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406–425.[PubMed]
    [Google Scholar]
  18. Skerman V. B. D..( 1967;). A Guide to the Identification of the Genera of Bacteria, , 2nd edn., Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  19. Tamura K., Dudley J., Nei M., Kumar S..( 2007;). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. . Mol Biol Evol 24: 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001092
Loading
/content/journal/ijsem/10.1099/ijsem.0.001092
Loading

Data & Media loading...

Supplementary File 1



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error