1887

Abstract

A Gram-stain-positive, catalase-positive, facultatively anaerobic, non-motile, coryneform bacterium, designated strain 32, was isolated from a closed pus sample from a patient having finger necrosis in Korea. Strain 32 was considered as representing a novel species according to its initial identification by matrix-assisted laser desorption/ionization-time-of-flight MS. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 32belonged to the genus and was closely related to DSM 7083 (=ATCC 49369) (98.34 % similarity). Optimal growth was observed at 30–40 °C and pH 7. Growth occurred in the presence of 0–6 % (w/v) NaCl. Menaquinones MK-8, MK-7 and MK-9 were the major respiratory quinones. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, glycolipid and two unknown lipids. The major cellular fatty acids were anteiso-C, anteiso-C, iso-C and iso-C. The DNA G+C content of strain 32 was 62.58 mol%, and the mean level of DNA–DNA relatedness between strain 32and ATCC 49369 was 49±1.6 %. Based on the phenotypic and genotypic characteristics, strain 32 is confirmed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 32(=NCCP 16133=DSM 101003).

Keyword(s): Dermabacter and new taxa
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2016-07-01
2020-04-02
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References

  1. Bavbek M., Caner H., Arslan H., Demirhan B., Tunçbilek S., Altinörs N.. 1998; Cerebral Dermabacter hominis abscess. Infection26:181–183 [CrossRef][PubMed]
    [Google Scholar]
  2. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric Deoxyribonucleic acid-Deoxyribonucleic acid Hybridization in Microdilution wells as an alternative to membrane filter Hybridization in which Radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39:224–229 [CrossRef]
    [Google Scholar]
  5. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  6. Funke G., Stubbs S., Pfyffer G. E., Marchiani M., Collins M. D.. 1994; Characteristics of CDC group 3 and group 5 coryneform bacteria isolated from clinical specimens and assignment to the genus Dermabacter. J Clin Microbiol32:1223–1228[PubMed]
    [Google Scholar]
  7. Gruner E., Steigerwalt A. G., Hollis D. G., Weyant R. S., Weaver R. E., Moss C. W., Daneshvar M., Brenner D. J.. 1994; Recognition of Dermabacter hominis, formerly CDC fermentative coryneform group 3 and group 5, as a potential human pathogen. J Clin Microbiol32:1918–1922[PubMed]
    [Google Scholar]
  8. Gómez-Garcés J. L., Oteo J., García G., Aracil B., Alós J. I., Funke G.. 2001; Bacteremia by Dermabacter hominis, a rare pathogen. J Clin Microbiol39:2356–2357 [CrossRef][PubMed]
    [Google Scholar]
  9. Hollis D. G.. 1992; Potential new CDC coryneform groups. Presented at the 92nd General Meeting of the American Society for Microbiology26–30 New Orleans, LA, USA: American Society for Microbiology;
    [Google Scholar]
  10. Jones D., Collins M. D.. 1988; Taxonomic studies on some human cutaneous coryneform bacteria: Description of Dermabacter hominis gen.nov., sp.nov. FEMS Microbiology Letters51:51–55 [CrossRef]
    [Google Scholar]
  11. Kim S. R., Rhee M. S., Kim B. C., Kim K. H.. 2007; Modeling the inactivation of Escherichia coli O157:H7 and generic Escherichia coli by supercritical carbon dioxide. Int J Food Microbiol118:52–61 [CrossRef][PubMed]
    [Google Scholar]
  12. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp115–175 Edited by Stackebrandt. E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  13. Lee H.-J., Cho C.-H., Kwon M.-J., Nam M.-H., Lee K.-N., Lee C.-K.. 2011; A patient with fatal septicemia caused by a rare pathogen dermabacter hominis. Infection and Chemotherapy43:86–88 [CrossRef]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of Deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriopl39:159–167 [CrossRef]
    [Google Scholar]
  15. Minnikin D. E., Collins M. D., Goodfellow M.. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol47:87–95 [CrossRef]
    [Google Scholar]
  16. Radtke A., Bergh K., Øien C. M., Bevanger L. S.. 2001; Peritoneal dialysis-associated peritonitis caused by Dermabacter hominis. J Clin Microbiol39:3420–3421 [CrossRef][PubMed]
    [Google Scholar]
  17. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  18. Skerman V. B. D.. 1967; A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  19. Tamura K., Dudley J., Nei M., Kumar S.. 2007; MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
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