1887

Abstract

A Gram-stain-negative, non-flagellated, non-gliding, non-spore-forming bacterial strain, designated YKTF-3, was isolated from a tidal flat on the Yellow Sea, South Korea. Strain YKTF-3 grew optimally at 30 °C and in the presence of 2.0–4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain YKTF-3 fell within the clade comprising the type strains of species. The novel strain exhibited 16S rRNA gene sequence similarity values of 97.22–98.34 % to the type strains of , , , , and , and of 95.08–96.95 % to the type strains of the other species. Strain YKTF-3 contained MK-6 as the predominant menaquinone and iso-C, summed feature 3 (C 7 and/or C 6) and iso-C 3-OH as the major fatty acids. The major polar lipids of strain YKTF-3 were phosphatidylethanolamine, one unidentified lipid, one unidentified aminophospholipid, one unidentified glycolipid and one unidentified aminolipid. The DNA G+C content was 32.3 mol% and its mean DNA–DNA relatedness values with the type strains of the six phylogenetically closely related species were 10–27 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain YKTF-3 is separate from other recognized species of the genus . On the basis of the data presented, strain YKTF-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YKTF-3 (=KCTC 52210=NBRC 111991).

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2016-07-01
2020-04-06
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References

  1. Barrow G. I., Feltham R. K. A.. 1993; Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press;[CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B.. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  3. Bernardet J.-F.. 2011; Family I. Flavobacteriaceae Reichenbach 1992. In Bergey’s Manual of Systematic Bacteriology, 2nd edn.vol. 4 pp106–111 Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig. W., Whitman W. B.. New York: Springer;
    [Google Scholar]
  4. Bowman J. P.. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of antarctic algae, and reclassification of zobell and upham 1944) reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol50:1861–1868 [CrossRef][PubMed]
    [Google Scholar]
  5. Bruns A., Rohde M., Berthe-Corti L.. 2001; Muricauda ruestringensis gen. nov ., sp. nov., a facultatively anaerobic, appendaged bacterium from german north sea intertidal sediment. syst evol microbiol. 511997–2006
  6. Choi D. H., Kim Y. G., Hwang C. Y., Yi H., Chun J., Cho B. C.. 2006; Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol56:635–640 [CrossRef][PubMed]
    [Google Scholar]
  7. Embley T. M., Wait R.. 1994; Structural lipids of eubacteria. In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics pp121–161 Goodfellow. M., O’Donnell A. G.. Chichester: John Wiley & Sons;
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39:224–229 [CrossRef]
    [Google Scholar]
  9. Frette L., Jørgensen N. O., Irming H., Kroer N.. 2004; Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in skagerrak, denmark. Int J Syst Evol Microbiol54:519–524 [CrossRef][PubMed]
    [Google Scholar]
  10. Heindl H., Wiese J., Imhoff J. F.. 2008; Tenacibaculum adriaticum sp. nov., from a bryozoan in the Adriatic Sea. Int J Syst Evol Microbiol58:542–547 [CrossRef][PubMed]
    [Google Scholar]
  11. Jung S. Y., Oh T. K., Yoon J. H.. 2006; Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in korea. Int J Syst Evol Microbiol56:1577–1581 [CrossRef][PubMed]
    [Google Scholar]
  12. Kang S. J., Lee S. Y., Lee M. H., Oh T. K., Yoon J. H.. 2012; Tenacibaculum geojense sp. nov., isolated from seawater. Int J Syst Evol Microbiol62:18–22 [CrossRef][PubMed]
    [Google Scholar]
  13. Kim Y. O., Park S., Nam B. H., Jung Y. T., Kim D. G., Jee Y. J., Yoon J. H.. 2013; Tenacibaculum halocynthiae sp. nov., a member of the family flavobacteriaceae isolated from sea squirt halocynthia roretzi. Antonie Van Leeuwenhoek103:1321–1327 [CrossRef][PubMed]
    [Google Scholar]
  14. Kim Y. O., Park I. S., Park S., Nam B. H., Park J. M., Kim D. G., Yoon J. H.. 2016; Tenacibaculum ascidiaceicola sp. nov., isolated from the golden sea squirt halocynthia aurantium . Int J Syst Evol Microbiol66:1174–1179[CrossRef]
    [Google Scholar]
  15. Komagata K., Suzuki K.-I.. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol19:161–207 [CrossRef]
    [Google Scholar]
  16. Lanyi B.. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol19:1–67 [CrossRef]
    [Google Scholar]
  17. Lee Y. S., Baik K. S., Park S. Y., Kim E. M., Lee D. H., Kahng H. Y., Jeon C. O., Jung J. S.. 2009; Tenacibaculum crassostreae sp. nov., isolated from the pacific oyster, Crassostrea gigas . Int J Syst Evol Microbiol59:1609–1614 [CrossRef][PubMed]
    [Google Scholar]
  18. Leifson E.. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol85:1183–1184
    [Google Scholar]
  19. Li Y., Wei J., Yang C., Lai Q., Chen Z., Li D., Zhang H., Tian Y., Zheng W., Zheng T.. 2013; Tenacibaculum xiamenense sp. nov., an algicidal bacterium isolated from coastal seawater. Int J Syst Evol Microbiol63:3481–3486 [CrossRef][PubMed]
    [Google Scholar]
  20. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  21. Oh Y. S., Kahng H. Y., Lee D. H., Lee S. B.. 2012; Tenacibaculum jejuense sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol62:414–419 [CrossRef][PubMed]
    [Google Scholar]
  22. Park S., Yoon J. H.. 2013; Tenacibaculum caenipelagi sp. nov., a member of the family flavobacteriaceae isolated from tidal flat sediment. Antonie Van Leeuwenhoek104:225–231 [CrossRef][PubMed]
    [Google Scholar]
  23. Park S., Park D. S., Bae K. S., Yoon J. H.. 2014; Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol64:1378–1383 [CrossRef][PubMed]
    [Google Scholar]
  24. Parte A. C.. 2014; LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res42:D613–D616 [CrossRef][PubMed]
    [Google Scholar]
  25. Piñeiro-Vidal M., Riaza A., Santos Y.. 2008; Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. Int J Syst Evol Microbiol58:21–25 [CrossRef][PubMed]
    [Google Scholar]
  26. Piñeiro-Vidal M., Gijón D., Zarza C., Santos Y.. 2012; Tenacibaculum dicentrarchi sp. nov., a marine bacterium of the family flavobacteriaceae isolated from european sea bass. Int J Syst Evol Microbiol62:425–429 [CrossRef][PubMed]
    [Google Scholar]
  27. Reichenbach H.. 1992; The order Cytophagales. In The Prokaryotes. a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn. pp.3631–3675 Balows A., Trüper H. G., Dworkin M., Harder. W., Schleifer K. H.. New York: Springer;
    [Google Scholar]
  28. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI Inc;
    [Google Scholar]
  29. Sheu S. Y., Lin K. Y., Chou J. H., Chang P. S., Arun A. B., Young C. C., Chen W. M.. 2007; Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. Int J Syst Evol Microbiol57:1148–1153 [CrossRef][PubMed]
    [Google Scholar]
  30. Stackebrandt E., Goebel B. M.. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol44:846–849 [CrossRef]
    [Google Scholar]
  31. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S.. 2001; Phylogenetic analysis and taxonomic study of marine cytophaga-like bacteria: Proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and T enacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol51:1639–1652[CrossRef]
    [Google Scholar]
  32. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  33. Wang J. T., Chou Y. J., Chou J. H., Chen C. A., Chen W. M.. 2008; Tenacibaculum aiptasiae sp. nov., isolated from a sea anemone aiptasia pulchella. Int J Syst Evol Microbiol58:761–766[CrossRef]
    [Google Scholar]
  34. Wayne L. G., Colwell R. R., Brenner D. J.. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464 [CrossRef]
    [Google Scholar]
  35. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H.. 1996; Identification of saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol46:502–505[CrossRef]
    [Google Scholar]
  36. Yoon J. H., Lee S. T., Park Y. H.. 1998; Inter- and intraspecific phylogenetic analysis of the genus nocardioides and related taxa based on 16S rRNA sequences. Int J Syst Bacteriol48:187–194[CrossRef]
    [Google Scholar]
  37. Yoon J. H., Kim I. G., Shin D. Y., Kang K. H., Park Y. H.. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a korean salt marsh. Int J Syst Evol Microbiol53:53–57 [CrossRef][PubMed]
    [Google Scholar]
  38. Yoon J. H., Kang S. J., Oh T. K.. 2005; Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the yellow sea, Korea. Int J Syst Evol Microbiol55:793–798 [CrossRef][PubMed]
    [Google Scholar]
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