1887

Abstract

A Gram-stain-negative, non-flagellated, non-gliding, non-spore-forming bacterial strain, designated YKTF-3, was isolated from a tidal flat on the Yellow Sea, South Korea. Strain YKTF-3 grew optimally at 30 °C and in the presence of 2.0–4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain YKTF-3 fell within the clade comprising the type strains of Tenacibaculumspecies. The novel strain exhibited 16S rRNA gene sequence similarity values of 97.22–98.34 % to the type strains of Tenacibaculum gallaicum, Tenacibaculum ascidiaceicola , Tenacibaculum litoreum, Tenacibaculum discolor , Tenacibaculum aestuarii and Tenacibaculum lutimaris, and of 95.08–96.95 % to the type strains of the other Tenacibaculum species. Strain YKTF-3 contained MK-6 as the predominant menaquinone and iso-C15 : 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids of strain YKTF-3 were phosphatidylethanolamine, one unidentified lipid, one unidentified aminophospholipid, one unidentified glycolipid and one unidentified aminolipid. The DNA G+C content was 32.3 mol% and its mean DNA–DNA relatedness values with the type strains of the six phylogenetically closely relatedTenacibaculum species were 10–27 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain YKTF-3 is separate from other recognized species of the genus Tenacibaculum . On the basis of the data presented, strain YKTF-3 is considered to represent a novel species of the genus Tenacibaculum , for which the name Tenacibaculum sediminilitoris sp. nov. is proposed. The type strain is YKTF-3 (=KCTC 52210=NBRC 111991).

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2016-07-01
2019-10-20
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References

  1. Barrow G. I. , Feltham R. K. A. . ( 1993;). Cowan and Steel's Manual for the Identification of Medical Bacteria, , 3rd edn., Cambridge:: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  2. Bernardet J.-F. , Nakagawa Y. , Holmes B. . Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52: 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bernardet J.-F. . ( 2011;). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,vol. 4 pp. 106–111. Krieg N. R. , Staley J. T. , Brown D. R. , Hedlund B. P. , Paster B. J. , Ward N. L. , Ludwig. W. , Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  4. Bowman J. P. . ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of antarctic algae, and reclassification of zobell and upham 1944) reichenbach 1989 as Cellulophaga uliginosa comb. nov. . Int J Syst Evol Microbiol 50: 1861–1868. [CrossRef] [PubMed]
    [Google Scholar]
  5. Bruns A. , Rohde M. , Berthe-Corti L. . ( 2001;). Muricauda ruestringensis gen. nov ., sp. nov., a facultatively anaerobic, appendaged bacterium from german north sea intertidal sediment. syst evol microbiol. .51 1997–2006.
  6. Choi D. H. , Kim Y. G. , Hwang C. Y. , Yi H. , Chun J. , Cho B. C. . ( 2006;). Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 56: 635–640. [CrossRef] [PubMed]
    [Google Scholar]
  7. Embley T. M. , Wait R. . ( 1994;). Structural lipids of eubacteria. . In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 121–161. Goodfellow. M. , O’Donnell A. G. . Chichester:: John Wiley & Sons;.
    [Google Scholar]
  8. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39: 224–229. [CrossRef]
    [Google Scholar]
  9. Frette L. , Jørgensen N. O. , Irming H. , Kroer N. . ( 2004;). Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in skagerrak, denmark. . Int J Syst Evol Microbiol 54: 519–524. [CrossRef] [PubMed]
    [Google Scholar]
  10. Heindl H. , Wiese J. , Imhoff J. F. . ( 2008;). Tenacibaculum adriaticum sp. nov., from a bryozoan in the Adriatic Sea. . Int J Syst Evol Microbiol 58: 542–547. [CrossRef] [PubMed]
    [Google Scholar]
  11. Jung S. Y. , Oh T. K. , Yoon J. H. . ( 2006;). Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in korea. . Int J Syst Evol Microbiol 56: 1577–1581. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kang S. J. , Lee S. Y. , Lee M. H. , Oh T. K. , Yoon J. H. . ( 2012;). Tenacibaculum geojense sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 62: 18–22. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kim Y. O. , Park S. , Nam B. H. , Jung Y. T. , Kim D. G. , Jee Y. J. , Yoon J. H. . ( 2013;). Tenacibaculum halocynthiae sp. nov., a member of the family flavobacteriaceae isolated from sea squirt halocynthia roretzi. . Antonie Van Leeuwenhoek 103: 1321–1327. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kim Y. O. , Park I. S. , Park S. , Nam B. H. , Park J. M. , Kim D. G. , Yoon J. H. . ( 2016;). Tenacibaculum ascidiaceicola sp. nov., isolated from the golden sea squirt halocynthia aurantium . . Int J Syst Evol Microbiol 66: 1174–1179.[CrossRef]
    [Google Scholar]
  15. Komagata K. , Suzuki K.-I. . ( 1987;). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19: 161–207. [CrossRef]
    [Google Scholar]
  16. Lanyi B. . ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19: 1–67. [CrossRef]
    [Google Scholar]
  17. Lee Y. S. , Baik K. S. , Park S. Y. , Kim E. M. , Lee D. H. , Kahng H. Y. , Jeon C. O. , Jung J. S. . ( 2009;). Tenacibaculum crassostreae sp. nov., isolated from the pacific oyster, Crassostrea gigas . . Int J Syst Evol Microbiol 59: 1609–1614. [CrossRef] [PubMed]
    [Google Scholar]
  18. Leifson E. . ( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85: 1183–1184.
    [Google Scholar]
  19. Li Y. , Wei J. , Yang C. , Lai Q. , Chen Z. , Li D. , Zhang H. , Tian Y. , Zheng W. , Zheng T. . ( 2013;). Tenacibaculum xiamenense sp. nov., an algicidal bacterium isolated from coastal seawater. . Int J Syst Evol Microbiol 63: 3481–3486. [CrossRef] [PubMed]
    [Google Scholar]
  20. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2: 233–241. [CrossRef]
    [Google Scholar]
  21. Oh Y. S. , Kahng H. Y. , Lee D. H. , Lee S. B. . ( 2012;). Tenacibaculum jejuense sp. nov., isolated from coastal seawater. . Int J Syst Evol Microbiol 62: 414–419. [CrossRef] [PubMed]
    [Google Scholar]
  22. Park S. , Yoon J. H. . ( 2013;). Tenacibaculum caenipelagi sp. nov., a member of the family flavobacteriaceae isolated from tidal flat sediment. . Antonie Van Leeuwenhoek 104: 225–231. [CrossRef] [PubMed]
    [Google Scholar]
  23. Park S. , Park D. S. , Bae K. S. , Yoon J. H. . ( 2014;). Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. . Int J Syst Evol Microbiol 64: 1378–1383. [CrossRef] [PubMed]
    [Google Scholar]
  24. Parte A. C. . ( 2014;). LPSN-list of prokaryotic names with standing in nomenclature. . Nucleic Acids Res 42: D613–D616. [CrossRef] [PubMed]
    [Google Scholar]
  25. Piñeiro-Vidal M. , Riaza A. , Santos Y. . ( 2008;). Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. . Int J Syst Evol Microbiol 58: 21–25. [CrossRef] [PubMed]
    [Google Scholar]
  26. Piñeiro-Vidal M. , Gijón D. , Zarza C. , Santos Y. . ( 2012;). Tenacibaculum dicentrarchi sp. nov., a marine bacterium of the family flavobacteriaceae isolated from european sea bass. . Int J Syst Evol Microbiol 62: 425–429. [CrossRef] [PubMed]
    [Google Scholar]
  27. Reichenbach H. . ( 1992;). The order Cytophagales. . In The Prokaryotes. a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, , 2nd edn., pp. 3631–3675. Balows A. , Trüper H. G. , Dworkin M. , Harder. W. , Schleifer K. H. . New York:: Springer;.
    [Google Scholar]
  28. Sasser M. . ( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, Newark, DE:: MIDI Inc;.
    [Google Scholar]
  29. Sheu S. Y. , Lin K. Y. , Chou J. H. , Chang P. S. , Arun A. B. , Young C. C. , Chen W. M. . ( 2007;). Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. . Int J Syst Evol Microbiol 57: 1148–1153. [CrossRef] [PubMed]
    [Google Scholar]
  30. Stackebrandt E. , Goebel B. M. . ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44: 846–849. [CrossRef]
    [Google Scholar]
  31. Suzuki M. , Nakagawa Y. , Harayama S. , Yamamoto S. . ( 2001;). Phylogenetic analysis and taxonomic study of marine cytophaga-like bacteria: Proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and T enacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. . Int J Syst Evol Microbiol 51: 1639–1652.[CrossRef]
    [Google Scholar]
  32. Tamaoka J. , Komagata K. . ( 1984;). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25: 125–128. [CrossRef]
    [Google Scholar]
  33. Wang J. T. , Chou Y. J. , Chou J. H. , Chen C. A. , Chen W. M. . ( 2008;). Tenacibaculum aiptasiae sp. nov., isolated from a sea anemone aiptasia pulchella. . Int J Syst Evol Microbiol 58: 761–766.[CrossRef]
    [Google Scholar]
  34. Wayne L. G. , Colwell R. R. , Brenner D. J. . ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37: 463–464. [CrossRef]
    [Google Scholar]
  35. Yoon J.-H. , Kim H. , Kim S.-B. , Kim H.-J. , Kim W. Y. , Lee S. T. , Goodfellow M. , Park Y.-H. . ( 1996;). Identification of saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46: 502–505.[CrossRef]
    [Google Scholar]
  36. Yoon J. H. , Lee S. T. , Park Y. H. . ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus nocardioides and related taxa based on 16S rRNA sequences. . Int J Syst Bacteriol 48: 187–194.[CrossRef]
    [Google Scholar]
  37. Yoon J. H. , Kim I. G. , Shin D. Y. , Kang K. H. , Park Y. H. . ( 2003;). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a korean salt marsh. . Int J Syst Evol Microbiol 53: 53–57. [CrossRef] [PubMed]
    [Google Scholar]
  38. Yoon J. H. , Kang S. J. , Oh T. K. . ( 2005;). Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the yellow sea, Korea. . Int J Syst Evol Microbiol 55: 793–798. [CrossRef] [PubMed]
    [Google Scholar]
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