1887

Abstract

A novel actinobacterial strain, designated R1-20, was isolated during a study of the bacterial diversity of the soil at a white heron nesting site. The isolate was non-motile, Gram-stain-positive and short rod-shaped. Colonies were dull white and convex with entire margin during the early stages of growth, and gradually became yellow. 16S rRNA gene sequence analysis indicated that the isolate belongs to the genus of the family , as sequence similarity with its nearest neighbours was 97.16 % with D7-27and 96.44 % with DSM 18994. However, the combination of cultural and physiological as well as chemotaxonomic properties clearly distinguished strain R1-20 from other species. The DNA G+C content of strain R1-20 was 65.5 mol%, and the major respiratory isoprenoid quinone was menaquinone MK-11. The acyl group of the peptidoglycan was of acetyl type, and the diagnostic diamino acid was 2,4-diaminobutyric acid. Glutamic acid, alanine and glycine were also present in the cell wall. The major fatty acids of strain R1-20 were anteiso-C, iso-C and anteiso-C. On the basis of phylogenetic, phenotypic and chemotaxonomic analysis, strain R1-20 merits recognition as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R1-20 (=KCTC 39614=JCM 31015).

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2016-07-01
2020-01-22
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