Full text loading...
Abstract
A Gram-reaction-positive, strictly aerobic, capsule-forming, motile and rod-shaped bacterium, designated strain XD80T, was isolated from the soil of a native cave in Lichuan, Hubei province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XD80T was most closely related to Domibacillus iocasae CCTCC AB 2015183T (98.66 % sequence similarity), followed by Domibacillus robiginosus DSM 25058T(97.83 %), Domibacillus tundrae KCTC 33549T (97.70 %), Domibacillus enclensis CCTCC AB 2011121T (97.21 %) and Domibacillus indicus DSM 28032T (96.96 %). Levels of DNA–DNA relatedness between strain XD80T and D. iocasae CCTCC AB 2015183T, D. robiginosusDSM 25058T, D. tundrae KCTC 33549T and D. enclensis CCTCC AB 2011121T were 37.4 %, 53.8 %, 53.6 % and 52.7 %, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipid and two unknown phospholipids. The predominant fatty acids (>5 %) were iso-C15 : 0 (37.3 %), anteiso-C15 : 0 (10.8 %), C16 : 0 (10.4 %), iso-C17 : 0 (10.3 %), C16 : 1ω11c (9.6 %) and anteiso-C17 : 0 (7.4 %). MK-6 (86.4 %) was the major respiratory quinone. The DNA G+C content was 46.4 mol%. The cell-wall peptidoglycan contained meso-diaminopimelic acid (type A1γ). Ribose and glucose were the major whole-cell sugars. In addition, strain XD80T showed differential physiological characteristics from most members of the genus Domibacillus , encompassing hydrolysis of starch, acid production from inositol and raffinose, and production of valine arylamidase. The results of this polyphasic study indicated that strain XD80T represents a novel species of the genus Domibacillus , for which the name Domibacillus antri sp. nov. is proposed. The type strain is XD80T (=CCTCC AB 2015053T=KCTC 33636T).
- Received:
- Accepted:
- Published Online: