1887

Abstract

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped bacterium, designated strain GYP-24, was isolated from the culture broth of a marine microalga, sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GYP-24 forms a robust cluster with KCTC 32177(95.8 % sequence similarity) in the family . Growth of strain GYP-24 was observed at 15, 22, 28, 30, 33 and 37 °C (optimal 30–33 °C), pH 6.0–10.0 (optimal pH 7.0–8.0) and in the presence of 0.5–4 % (w/v) NaCl (optimal 2–3 %). The only menaquinone of strain GYP-24 was MK-6, and the G+C content of the genomic DNA was 36.9 mol%. The major fatty acid profile comprised iso-C 3-OH, summed feature 3 (Cω7/ω6), iso-C G and iso-C. The major polar lipids of strain GYP-24 were phosphatidylethanolamine, one unidentified phospholipid, three unidentified aminolipids and three unidentified lipids. Comprehensive analyses based on polyphasic characterization of GYP-24 indicated that it represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is GYP-24(=KCTC 42903=MCCC 1K01730). An emended description of the genus is also given.

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2016-08-01
2020-03-28
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References

  1. Bernardet J. F., Nakagawa Y., Holmes B.. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  2. Chevanton M. L., Garnier M., Bougaran G., Schreiber N., Lukomska E., Bérard J.-B., Fouilland E., Bernard O., Cadoret J. P.. 2013; Screening and selection of growth-promoting bacteria for Dunaliella cultures. Algal Research2:212–222 [CrossRef]
    [Google Scholar]
  3. Cho D. H., Ramanan R., Heo J., Lee J., Kim B. H., Oh H. M., Kim H. S.. 2015; Enhancing microalgal biomass productivity by engineering a microalgal-bacterial community. Bioresour Technol175:578–585 [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D.. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp.345–401 Edited by Goodfellow M., O’Donnell A. G.. Chichester: John Wiley & Sons;
    [Google Scholar]
  5. Dong X. Z., Cai M. Y.. 2001; Determinative Manual for Routine Bacteriology Beijing: Scientific Press;
    [Google Scholar]
  6. Fandino L. B., Riemann L., Steward G. F., Long R. A., Azam F.. 2001; Variations in bacterial community structure during a dinoflagellate bloom analyzed by DGGE and 16S rDNA sequencing. Aquat Microb Ecol23:119–130 [CrossRef]
    [Google Scholar]
  7. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  9. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. (editors) 1994; Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Goodfellow M., Orchard V. A.. 1974; Antibiotic sensitivity of some nocardioform bacteria and its value as a criterion for taxonomy. J Gen Microbiol83:375–387 [CrossRef][PubMed]
    [Google Scholar]
  11. Gutierrez T., Green D. H., Whitman W. B., Nichols P. D., Semple K. T., Aitken M. D.. 2012; Algiphilus aromaticivorans gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium isolated from a culture of the marine dinoflagellate Lingulodinium polyedrum, and proposal of Algiphilaceae fam. nov. Int J Syst Evol Microbiol62:2743–2749 [CrossRef][PubMed]
    [Google Scholar]
  12. Gutierrez T., Green D. H., Nichols P. D., Whitman W. B., Semple K. T., Aitken M. D.. 2013; Polycyclovorans algicola gen. nov., sp. nov., an aromatic-hydrocarbon-degrading marine bacterium found associated with laboratory cultures of marine phytoplankton. Appl Environ Microbiol79:205–214 [CrossRef][PubMed]
    [Google Scholar]
  13. Jung Y. T., Lee J. S., Yoon J. H.. 2013; Hwangdonia seohaensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a tidal flat sediment. Int J Syst Evol Microbiol63:3186–3191 [CrossRef][PubMed]
    [Google Scholar]
  14. Kamekura M.. 1993; Lipids of extreme halophiles. In The Biology of Halophilic Bacteria pp135–161 Edited by Vreeland R. H., Hochstein L. I.. Boca Raton: CRC Press;
    [Google Scholar]
  15. Kim B. H., Ramanan R., Cho D. H., Oh H. M., Kim H. S.. 2014; Role of Rhizobium, a plant growth promoting bacterium, in enhancing algal biomass through mutualistic interaction. Biomass and Bioenergy69:95–105 [CrossRef]
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  17. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  18. Komagata K., Suzuki K.. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol19:161–207[CrossRef]
    [Google Scholar]
  19. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp.115–175 Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  20. Lichtenthaler H. K., Buschmann C.. 2001; Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. In Current Protocols in Food Analytical Chemistry pp.F4.3.1–F4.3.8 Edited by Wrolstad R. E., Acree T. E., Decker E. A., Penner M. H., Reid D. S., Schwartz S. J., Shoemaker C. F., Smith D. M., Sporns P.. New York: John Wiley & Sons;
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39:159–167 [CrossRef]
    [Google Scholar]
  22. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V.. 2003; Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum Cytophaga-Flavobacterium-Bacteroides . Int J Syst Evol Microbiol53:81–85 [CrossRef][PubMed]
    [Google Scholar]
  23. Pruesse E., Peplies J., Glöckner F. O.. 2012; SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics28:1823–1829 [CrossRef][PubMed]
    [Google Scholar]
  24. Rzhetsky A., Nei M.. 1992; A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol9:945–967
    [Google Scholar]
  25. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol4:406–425
    [Google Scholar]
  26. Swofford D. L.. 1993; PAUP: Phylogenetic Analysis Using Parsimony, Version 3.1.1 Champaign, IL: Illinois Natural History Survey;
    [Google Scholar]
  27. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  28. Tindall B. J.. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett66:199–202 [CrossRef]
    [Google Scholar]
  29. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R.. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology pp330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R.. Washington DC: American Society for Microbiology;
    [Google Scholar]
  30. Wang G., Fan J., Wu H., Zhang X., Li G., Zhang H., Yang X., Ye F., Xiang W. et al. 2013; Nonhongiella spirulinensis gen. nov., sp. nov., a bacterium isolated from a cultivation pond of Spirulina platensis in Sanya, China. Antonie Van Leeuwenhoek106:591–592 [CrossRef]
    [Google Scholar]
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