1887

Abstract

A Gram-stain-negative, aerobic, motile rod-shaped bacterium, designated 6-67, was isolated from a soil sample collected from Longyearbyen, Svalbard. Its taxonomic position was investigated by genotypic, phenotypic and chemotaxonomic analyses. This isolate grew at 4–28 °C (optimum, 20 °C), at pH 5.0–9.0 (optimum, pH 7.0) and with 0–0.2 % (w/v) NaCl. Strain 6-67 contained Q-8 as a major respiratory quinone and MK-7 as a minor component; the major fatty acids were summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and C16 : 0. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified phospholipids and one unidentified aminophospholipid. The polyamines were putrescine and 2-hydroxyputrescine. 16S rRNA gene sequence analysis indicated that the novel strain 6-67 belonged to the family Oxalobacteraceae within the class Betaproteobacteria . The DNA G+C content of strain 6-67 was 56.21 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain 6-67 is considered to represent a novel species of the genus Undibacterium , for which the name Undibacterium arcticum sp. nov. is proposed. The type strain is 6-67 (=CCTCC AB 2015162=KCTC 42986).

Erratum
This article contains a correction applying to the following content:
Undibacterium arcticum sp. nov., isolated from arctic alpine soil
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001056
2016-08-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/8/2797.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001056&mimeType=html&fmt=ahah

References

  1. Bauer A. W. , Kirby W. M. , Sherris J. C. , Turck M. . ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45: 493–496.
    [Google Scholar]
  2. Bowman J. P. . ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. . Int J Syst Evol Microbiol 50: 1861–1868.[CrossRef]
    [Google Scholar]
  3. Busse H.-J. , Auling G. . ( 1988;). Polyamine pattern as a chemotaxonomic marker within the proteobacteria. . Syst Appl Microbiol 11: 1–8.[CrossRef]
    [Google Scholar]
  4. Busse H.-J. , Bunka S. , Hensel A. , Lubitz W. . ( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. . Int J Syst Bacteriol 47: 698–708.[CrossRef]
    [Google Scholar]
  5. Cowan S. T. . ( 1965;). Development of coding schemes for microbial taxonomy. . Adv Appl Microbiol 7: 139–167.[CrossRef]
    [Google Scholar]
  6. Doetsch R. N. . ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology , pp. 21–33. Edited by Gerhardt P. , Murray R. G. E. , Costilow R. N. , Nester E. W. , Wood W. A. , Krieg N. R. , Phillips G. H. , Washington D. C. .
    [Google Scholar]
  7. Du J. , Akter S. , Won K. , Singh H. , Yin C. S. , Kook M. , Shik Yin C. , Yi T.-H. . ( 2015;). Undibacterium aquatile sp. nov., isolated from a waterfall. . Int J Syst Evol Microbiol 65: 4128–4133.[CrossRef]
    [Google Scholar]
  8. Eder W. , Wanner G. , Ludwig W. , Busse H.-J. , Ziemke-Kägeler F. , Lang E. . ( 2011;). Description of Undibacterium oligocarboniphilum sp. nov., isolated from purified water, and Undibacterium pigrum strain CCUG 49012 as the type strain of Undibacterium parvum sp. nov., and emended descriptions of the genus Undibacterium and the species Undibacterium pigrum . . Int J Syst Evol Microbiol 61: 384–391.[CrossRef]
    [Google Scholar]
  9. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17: 368–376.[CrossRef]
    [Google Scholar]
  10. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39: 783–791.[CrossRef]
    [Google Scholar]
  11. Fitch W. M. . ( 1971;). Towards defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20: 406–416.[CrossRef]
    [Google Scholar]
  12. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kim S. J. , Moon J. Y. , Weon H. Y. , Hong S. B. , Seok S. J. , Kwon S. W. . ( 2014;). Undibacterium jejuense sp. nov. and Undibacterium seohonense sp. nov., isolated from soil and freshwater, respectively. . Int J Syst Evol Microbiol 64: 236–241.[CrossRef]
    [Google Scholar]
  14. Kimura M. . ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16: 111–120.[CrossRef]
    [Google Scholar]
  15. Kovacs N. . ( 1956;). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178: 703.[CrossRef]
    [Google Scholar]
  16. Kämpfer P. , Rosselló-Mora R. , Hermansson M. , Persson F. , Huber B. , Falsen E. , Busse H. J. . ( 2007;). Undibacterium pigrum gen. nov., sp. nov., isolated from drinking water. . Int J Syst Evol Microbiol 57: 1510–1515.[CrossRef]
    [Google Scholar]
  17. Liu Y. Q. , Wang B. J. , Zhou N. , Liu S. J. . ( 2013;). Undibacterium terreum sp. nov., isolated from permafrost soil. . Int J Syst Evol Microbiol 63: 2296–2300.[CrossRef]
    [Google Scholar]
  18. Mesbah M. , Whitman W. B. . ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr 479: 297–306.[CrossRef]
    [Google Scholar]
  19. Murray R. G. E. , Doetsch R. N. , Robinow C. F. . ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology , pp. 21–41. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  20. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406–425.
    [Google Scholar]
  21. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  22. Sheu S. Y. , Lin Y. S. , Chen J. C. , Chen W. M. . ( 2014;). Undibacterium macrobrachii sp. nov., isolated from a freshwater shrimp culture pond. . Int J Syst Evol Microbiol 64: 1036–1042.[CrossRef]
    [Google Scholar]
  23. Sheu S. Y. , Lin Y. S. , Chen J. C. , Kwon S. W. , Chen W. M. . ( 2014;). Undibacterium squillarum sp. nov., isolated from a freshwater shrimp culture pond. . Int J Syst Evol Microbiol 64: 3459–3466.[CrossRef]
    [Google Scholar]
  24. Stackebrandt E. , Goebel B. M. . ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44: 846–849.[CrossRef]
    [Google Scholar]
  25. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28: 2731–2739.[CrossRef]
    [Google Scholar]
  26. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997;). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25: 4876–4882.[CrossRef]
    [Google Scholar]
  27. Tindall B. J. . ( 1990;). Lipid composition of Halobacterium lacusprofundi. . Microbiol Lett 66: 199–202.[CrossRef]
    [Google Scholar]
  28. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173: 697–703.
    [Google Scholar]
  29. Wilson K. H. , Blitchington R. B. , Greene R. C. . ( 1990;). Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction. . J Clin Microbiol 28: 1942–1946.
    [Google Scholar]
  30. Xie C. H. , Yokota A. . ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49: 345–349.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001056
Loading
/content/journal/ijsem/10.1099/ijsem.0.001056
Loading

Data & Media loading...

Supplements

Supplementary File 1



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error