1887

Abstract

Bacillus axarquiensis and Bacillus malacitensis were previously reported to be later heterotypic synonyms of Bacillus mojavensis , based primarily on DNA–DNA relatedness values. We have sequenced draft genomes of Bacillus axarquiensis NRRL B-41617 and Bacillus malacitensis NRRL B-41618. Comparative genomics and DNA–DNA relatedness calculations showed that while Bacillus axarquiensis and Bacillus malacitensis are synonymous with each other, they are not synonymous with Bacillus mojavensis . In addition, a draft genome was completed for Brevibacterium halotolerans , a strain long suspected of being a Bacillus subtilis group member based on 16S rRNA similarities (99.8 % with Bacillus mojavensis ). Comparative genomics and DNA–DNA relatedness calculations showed that Brevibacterium halotolerans is synonymous with Bacillus axarquiensis and Bacillus malacitensis . The pairwise in silico DNA–DNA hybridization values calculated in comparisons between the three conspecific strains were all greater than 92 %, which is well above the standard species threshold of 70 %. While the pairwise in silico DNA–DNA hybridization values calculated in comparisons of the three conspecific strains with Bacillus mojavensis were all less than 65 %. The combined results of our genotype and phenotype studies showed that Bacillus axarquiensis, Bacillus malacitensis , and Brevibacterium halotolerans are conspecific and distinct from Bacillus mojavensis . Because the valid publication of the name Bacillus axarquiensis predates the publication of the name Bacillus malacitensis , we propose that Bacillus malacitensis be reclassified as a synonym of Bacillus axarquiensis . In addition, we propose to reclassify Brevibacterium halotolerans as a synonym of Bacillus axarquiensis . An amended description of Bacillus axarquiensis is provided.

Keyword(s): bacillaene and nep1
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2016-06-10
2019-10-16
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