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Abstract

A novel bacterial strain designated MSr9521was isolated in 2014 from a soil sample collected in 1986 from the Philippines. The novel bacterium shows myxobacterial characteristics that include pseudoplasmodial swarming, fruiting body formation and predatory ability to lyse microorganisms. The strain is chemoheterotrophic, mesophilic and aerobic. Major fatty acids are CC2-OH and iso-C, and also contains trace amounts of omega-3/-6 polyunsaturated fatty acids. The G+C content of the genomic DNA is 70.4 mol%. The 16S rRNA gene sequence shows 95–96 % closest similarity to DSM 14627, Pl fu5, Pl t4 and By c2. The molecular phylogenetic analysis shows that the novel isolate forms a novel branch in the family , suborder S. Polyphasic taxonomic characterization suggests that the strain MSr9521represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is MSr9521 (=DSM 100773=NCCB 100574).

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2016-06-10
2020-04-05
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References

  1. Dawid W.. 2000; Biology and global distribution of myxobacteria in soils. FEMS Microbiol Rev24:403–427 [CrossRef][PubMed]
    [Google Scholar]
  2. Drummond A. J., Ashton B., Buxton S., Cheung M., Heled J., Kearse M., Moir R., Stones-Havas S., Sturrock S.. 2010; Geneious Pro 5.0.2. http://www.geneious.com
  3. Edgar R. C.. 2004; MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res32:1792–1797 [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J.. 1985; Confidence limits on phylogenies: An approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  5. Fudou R., Jojima Y., Iizuka T., Yamanaka S.. 2002; Haliangium ochraceum gen. nov., sp. nov. and haliangium tepidum sp. nov.: Novel moderately halophilic myxobacteria isolated from coastal saline environments. J Gen Appl Microbiol48:109–115 [CrossRef][PubMed]
    [Google Scholar]
  6. Garcia R., Gerth K., Stadler M., Dogma Jr, I. J., Müller R.. 2010; Expanded phylogeny of myxobacteria and evidence for cultivation of the ‘unculturables'. Mol Phylogenet Evol57:878–887 [CrossRef][PubMed]
    [Google Scholar]
  7. Garcia R., Pistorius D., Stadler M., Müller R.. 2011; Fatty acid-related phylogeny of myxobacteria as an approach to discover polyunsaturated omega-3/6 fatty acids. J Bacteriol193:1930–1942 [CrossRef][PubMed]
    [Google Scholar]
  8. Garcia R., Gemperlein K., Müller R.. 2014a; Minicystis rosea gen. nov., sp. nov., a polyunsaturated fatty acid-rich and steroid-producing soil myxobacterium. Int J Syst Evol Microbiol64:3733–3742 [CrossRef]
    [Google Scholar]
  9. Garcia R., Müller R.. 2014b; The Family Polyangiaceae . In The Prokaryotes Deltaproteobacteria and Epsilonproteobacteria, 4 edn. pp247–279 Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F.. Berlin, Heidelberg: Springer; [CrossRef]
    [Google Scholar]
  10. Garcia R. O., Krug D., Müller R.. 2009; Chapter 3. discovering natural products from myxobacteria with emphasis on rare producer strains in combination with improved analytical methods. Methods Enzymol458:59–91 [CrossRef][PubMed]
    [Google Scholar]
  11. Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.. (editors). 1981; Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Germperlein K., Rachid S., Garcia R. O., Wenzel S. C., Müller R.. 2014; Polyunsaturated fatty acid biosynthesis in myxobacteria: Different PUFA synthases and their product diversity. Chem Sci5:1733–1741 [CrossRef]
    [Google Scholar]
  13. Guindon S., Gascuel O.. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol52:696–704 [CrossRef][PubMed]
    [Google Scholar]
  14. Hall T. A.. 1999; BIOEDIT: A user-friendly biological sequence alignment editor and analysis program from Windows 95/98/NT. Nucleic Acids Symp41:95–98
    [Google Scholar]
  15. Higgs P. I, Hartzell P. L., Holkenbrink C., Hoiczyk E.. 2014; Myxococcus xanthus vegetative and developmental cell heterogeneity. In Myxobacteria: Genomics, Cellular and Molecular Biology pp51–77 Edited by Yang Z., Higgs P.. UK: Caister Academic;
    [Google Scholar]
  16. Iizuka T., Jojima Y., Fudou R., Hiraishi A., Ahn J. W., Yamanaka S.. 2003a; Plesiocystis pacifica gen. nov., sp. nov., a marine myxobacterium that contains dehydrogenated menaquinone, isolated from the pacific coasts of Japan. Int J Syst Evol Microbiol53:183–195 [CrossRef]
    [Google Scholar]
  17. Iizuka T., Jojima Y., Fudou R., Tokura M., Hiraishi A., Yamanaka S.. 2003b; Enhygromyxa salina gen. nov., sp. nov., a slightly halophilic myxobacterium isolated from the coastal areas of Japan. Syst Appl Microbiol26:189–196 [CrossRef]
    [Google Scholar]
  18. Jukes T. H., Cantor C. R.. 1969; Evolution of protein molecules. In Mammalian Protein Metabolismvol. 3 pp21–132 Edited by Munro H. N.. New York: Academic Press; [CrossRef]
    [Google Scholar]
  19. Lachnik J., Ackermann B., Bohrssen A., Maass S., Diephaus C., Puncken A., Stermann M., Bange F. C.. 2002; Rapid-cycle PCR and fluorimetry for detection of mycobacteria. J Clin Microbiol40:3364–3373 [CrossRef][PubMed]
    [Google Scholar]
  20. Lane D. J.. 1991; 16S/23S rDNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp.115–175 Stackebrandt E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  21. Lang E., Kroppenstedt R. M., Sträubler B., Stackebrandt E.. 2008; Reclassification of myxococcus flavescens yamanaka et al. 1990VP as a later synonym of myxococcus virescens thaxter 1892AL . Int J Syst Evol Microbiol58:2607–2609 [CrossRef][PubMed]
    [Google Scholar]
  22. Li G., Shimelis O., Zhou, X, Giese R. W.. 2003; Scaled-down nuclease p1 for scaled-up DNA digestion. Biotechnique34:908–909
    [Google Scholar]
  23. McCurdy H. D.. 1969; Studies on the taxonomy of the Myxobacterales. I. Record of Canadian isolates and survey of methods. Can J Microbiol15:1453–1461 [CrossRef][PubMed]
    [Google Scholar]
  24. Morgulis A., Coulouris G., Raytselis Y., Madden T. L., Agarwala R., Schäffer A. A.. 2008; Database indexing for production megablast searches. Bioinformatics24:1757–1764 [CrossRef][PubMed]
    [Google Scholar]
  25. Plaza A., Müller R.. 2014; Chapter 6, Myxobacteria: Chemical diversity and screening strategies. In Natural Products: Discourse, Diversity, and Design, 1st edn. pp.103–124 Edited by Osbourn A., Goss R. J., Carter G. T.. Hoboken, NJ: John Wiley & Sons, Inc;[CrossRef]
    [Google Scholar]
  26. Reichenbach H., Dworkin M.. 1992; The myxobacteria. In The Prokaryotes, 2nd edn. pp.3416–3487 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H.. Berlin: Springer; [CrossRef]
    [Google Scholar]
  27. Reichenbach H.. 2005; Order VIII. Myxococcales Tchan, Pochon and Prévot 1948, 398AL . In Bergey’s Manual of Systematic Bacteriology, 2nd edn.vol. 2C pp1059–1072 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M.. New York: Springer;
    [Google Scholar]
  28. Reichenbach H.. 2006a; The genus Lysobacter . In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn.vol. 6 pp939–957 Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E.. New York: Springer; [CrossRef]
    [Google Scholar]
  29. Reichenbach H., Lang E., Schumann P., Spröer C.. 2006b; Byssovorax cruenta gen. nov., sp. nov., nom. rev., a cellulose-degrading myxobacterium: Rediscovery of ‘myxococcus cruentus' thaxter 1897. Int J Syst Evol Microbiol56:2357–2363 [CrossRef]
    [Google Scholar]
  30. Saitou N., Nei M.. 1987; The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  31. Shimelis O., Giese R. W.. 2006; Nuclease P1 digestion/high-performance liquid chromatography, a practical method for DNA quantitation. J Chromatogr A1117:132–136 [CrossRef][PubMed]
    [Google Scholar]
  32. Sneath, P. H. A, Sokal, R. R. 1973; Unweighted pair group method with arithmetic mean. In Numerical Taxonomy pp230–234 San Francisco: Freeman;
    [Google Scholar]
  33. Weissman K. J., Müller R.. 2010; Myxobacterial secondary metabolites: Bioactivities and modes-of-action. Nat Prod Rep27:1276–1295 [CrossRef][PubMed]
    [Google Scholar]
  34. Wenzel S. C., Müller R.. 2009; The biosynthetic potential of myxobacteria and their impact in drug discovery. Curr Opin Drug Discov Devel12:220–230[PubMed]
    [Google Scholar]
  35. Zhang Z., Schwartz S., Wagner L., Miller W.. 2000; A greedy algorithm for aligning DNA sequences. J Comput Biol7:203–214 [CrossRef][PubMed]
    [Google Scholar]
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