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Abstract

A bacterial strain, designated JN14-9, was isolated from surface sediment of the Jiulong River and characterized in a taxonomic study using a polyphasic approach. Strain JN14-9 was Gram-stain-negative, non-motile, rod-shaped and orange-pigmented. It can grow at 15–37 °C (optimum 25–30 °C), at pH 7–8 (optimum pH 7) and in 2–6 % (w/v) NaCl (optimum 3–4 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain JN14-9 formed an independent lineage related to the family Cytophagaceae with low 16S rRNA gene sequence similarities (<92.5 %) to members of other genera with validly published names. The principal fatty acids of strain JN14-9 were summed feature 3 (C16:1ω6c/C16:1ω7c) and iso-C15:0. The isoprenoid quinone was identified as MK-7. The major polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, three aminophospholipids and five unidentified phospholipids. The DNA G+C content was 41.6 mol%. Results of phenotypic, phylogenetic and chemotaxonomic analyses clearly indicate that strain JN14-9 represents a novel species of a new genus within the family Cytophagaceae , for which the name Jiulongibacter sediminis gen. nov., sp. nov. is proposed. The type strain of the type species is JN14-9 (=MCCC 1A00733=KCTC 42153).

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2016-06-10
2019-10-20
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