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Abstract

A novel Gram-stain-negative, rod-shaped (1.0–1.2×2.0–8.0 µm), non-motile without flagella strain, designated HSF7, was isolated from deep seawater. Strain HSF7 was able to grow at 20–40 °C (optimum 35 °C), pH 5.5–9.0 (optimum pH 6.5) and 0–10 % (w/v) NaCl (optimum 2 %). The G+C content of the genomic DNA was 69 mol%. Bacteriochlorophyll a and poly-β-hydroxybutyrate (PHB) granules were not found. The major fatty acids were C18 : 1ω7c (69.3 %), C16 : 0 (9.1 %) and C19 : 0 cyclo ω8c (6.6 %). The polar lipids were phosphatidylglycerol, three unknown aminophospholipids, an unknown phospholipid, an unknown aminolipid and two unknown lipids. The only isoprenoid quinone was Q-10. 16S rRNA gene sequence analysis revealed that strain HSF7 was most closely related toThalassobaculum salexigens DSM 19539, Thalassobaculum litoreum DSM 18839, Nisaeadenitrificans DSM 18348 and Oceanibaculum indicum MCCC 1A02083with pairwise sequence similarities of 95.56 %, 95.21 %, 93.64 % and 92.65 %, respectively. On the basis of genotypic, phenotypic, phylogenetic and chemotaxonomic characteristics, strain HSF7 represents a novel species of the genus Thalassobaculum , or which the name Thalassobaculum fulvum sp. nov. is proposed. The type strain is HSF7(=KCTC 42651=MCCC 1K01158).

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2016-06-10
2019-10-22
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