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Abstract

A novel Gram-stain-negative, rod-shaped (1.0–1.2×2.0–8.0 µm), non-motile without flagella strain, designated HSF7, was isolated from deep seawater. Strain HSF7 was able to grow at 20–40 °C (optimum 35 °C), pH 5.5–9.0 (optimum pH 6.5) and 0–10 % (w/v) NaCl (optimum 2 %). The G+C content of the genomic DNA was 69 mol%. Bacteriochlorophyll and poly--hydroxybutyrate (PHB) granules were not found. The major fatty acids were Cω7 (69.3 %), C (9.1 %) and C cyclo ω8 (6.6 %). The polar lipids were phosphatidylglycerol, three unknown aminophospholipids, an unknown phospholipid, an unknown aminolipid and two unknown lipids. The only isoprenoid quinone was Q-10. 16S rRNA gene sequence analysis revealed that strain HSF7 was most closely related to DSM 19539, DSM 18839, DSM 18348 and MCCC 1A02083with pairwise sequence similarities of 95.56 %, 95.21 %, 93.64 % and 92.65 %, respectively. On the basis of genotypic, phenotypic, phylogenetic and chemotaxonomic characteristics, strain HSF7 represents a novel species of the genus , or which the name sp. nov. is proposed. The type strain is HSF7(=KCTC 42651=MCCC 1K01158).

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2016-06-10
2021-08-02
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