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Abstract

A Gram-stain-negative, aerobic, non-flagellated, non-gliding and ovoid or rod-shaped bacterium, designated DB-3, was isolated from a tidal flat on the Yellow Sea in South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain DB-3 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain DB-3 fell within the clade comprising the type strains of species of the genus . Strain DB-3 exhibited 16S rRNA gene sequence similarities of 93.2–96.9 % to the type strains of species of the genus . Strain DB-3 contained MK-6 as the predominant menaquinone and iso-C, anteiso-C and iso-C 3-OH as the major fatty acids. The major or significant amounts of polar lipids detected in strain DB-3 were phosphatidylethanolamine, an unidentified aminolipid and seven unidentified lipids. The DNA G+C content was 34.7 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, revealed that strain DB-3 is separated from recognized species of the genus . On the basis of the data presented, strain DB-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DB-3 (=KCTC 52037=NBRC 111757).

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2016-06-10
2020-04-07
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References

  1. Barrow G. I., Feltham R. K. A.. 1993; Cowan and Steel’s Manual for the Identification of Medical Bacteria. , 3rd edn. Cambridge: Cambridge University Press;
  2. Bernardet J.-F.. 2011; Family I. Flavobacteriaceae Reichenbach 1992. In Bergey’s Manual of Systematic Bacteriology, 2nd edn.vol. 4 pp.106–111 Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B.. New York: Springer;
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B.. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  4. Bowman J. P., McCammon S. A., Lewis T., Skerratt J. H., Brown J. L., Nichols D. S., McMeekin T. A.. 1998; Psychroflexus torquisgen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense (Dobson et al. 1993) as Psychroflexus gondwanense gen. nov., comb. nov. Microbiology144:1601–1609 [CrossRef][PubMed]
    [Google Scholar]
  5. Bowman J. P.. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol50:1861–1868 [CrossRef][PubMed]
    [Google Scholar]
  6. Bruns A., Rohde M., Berthe-Corti L.. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol51:1997–2006 [CrossRef][PubMed]
    [Google Scholar]
  7. Chen Y. G., Cui X. L., Wang Y. X., Tang S. K., Zhang Y. Q., Li W. J., Liu J. H., Peng Q., Xu L. H.. 2009; Psychroflexus sediminis sp. nov., a mesophilic bacterium isolated from salt lake sediment in China. Int J Syst Evol Microbiol59:569–573 [CrossRef][PubMed]
    [Google Scholar]
  8. Chun J., Kang J. Y., Jahng K. Y.. 2014; Psychroflexus salarius sp. nov., isolated from Gomso salt pan. Int J Syst Evol Microbiol64:3467–3472 [CrossRef][PubMed]
    [Google Scholar]
  9. Dobson S. J., Colwell R. R., McMeekin T. A., Franzmann P. D.. 1993; Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol43:77–83 [CrossRef][PubMed]
    [Google Scholar]
  10. Donachie S. P., Bowman J. P., Alam M.. 2004; Psychroflexus tropicus sp. nov., an obligately halophilic Cytophaga–Flavobacterium–Bacteroides group bacterium from an Hawaiian hypersaline lake. Int J Syst Evol Microbiol54:935–940 [CrossRef][PubMed]
    [Google Scholar]
  11. Embley T. M., Wait R.. 1994; Structural lipids of eubacteria. In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics pp.121–161 Edited by Goodfellow M., O’Donnell A. G.. Chichester: Wiley;
    [Google Scholar]
  12. Komagata K., Suzuki K.. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol19:161–207 [CrossRef]
    [Google Scholar]
  13. Leifson E.. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol85:1183–1184[PubMed]
    [Google Scholar]
  14. Lányí B.. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol19:1–67 [CrossRef]
    [Google Scholar]
  15. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  16. Park S., Park D. S., Bae K. S., Yoon J. H.. 2014; Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol64:1378–1383 [CrossRef][PubMed]
    [Google Scholar]
  17. Reichenbach H.. 1992; The order Cytophagales . In The Prokaryotes. a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn. pp.3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H.. New York, NY: Springer;
    [Google Scholar]
  18. Sasser M.. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  19. Seiler H., Bleicher A., Busse H. J., Hüfner J., Scherer S.. 2012; Psychroflexus halocasei sp. nov., isolated from a microbial consortium on a cheese. Int J Syst Evol Microbiol62:1850–1856 [CrossRef][PubMed]
    [Google Scholar]
  20. Stackebrandt E., Goebel B. M.. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol44:846–849[CrossRef]
    [Google Scholar]
  21. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  22. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H.. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol46:502–505 [CrossRef]
    [Google Scholar]
  23. Yoon J. H., Lee S. T., Park Y. H.. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol48:Pt 1187–194 [CrossRef][PubMed]
    [Google Scholar]
  24. Yoon J. H., Kang K. H., Park Y. H.. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional korean fermented seafood. Int J Syst Evol Microbiol53:449–454 [CrossRef][PubMed]
    [Google Scholar]
  25. Yoon J. H., Kang S. J., Jung Y. T., Oh T. K.. 2009; Psychroflexus salinarum sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol59:2404–2407 [CrossRef][PubMed]
    [Google Scholar]
  26. Zhang H., Hosoi-Tanabe S., Nagata S., Ban S., Imura S.. 2010; Psychroflexus lacisalsi sp. nov., a moderate halophilic bacterium isolated from a hypersaline lake (Hunazoko-Ike) in Antarctica. J Microbiol48:160–164 [CrossRef][PubMed]
    [Google Scholar]
  27. Zhong Z.-P., Liu Y., Wang F., Zhou Y.-G., Liu H.-C., Liu Z.-P.. 2016; Psychroflexus salis sp. nov. and Psychroflexus planctonicus sp. nov. isolated from a salt lake. Int J Syst Evol Microbiol66:125–131[CrossRef]
    [Google Scholar]
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