1887

Abstract

A Gram-staining-negative bacterium, strain X14M-14, was isolated from a salt lake (Lake Xiaochaidan) in Qaidam basin, Qinghai Province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain X14M-14 were non-spore-forming, non-motile rods. Strain X14M-14 was strictly heterotrophic and aerobic, catalase-positive and oxidase-negative. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain X14M-14 belonged to the family and formed a distinct lineage that was independent of the most closely related genera: (16S rRNA gene sequence similarities, 91.8–93.1 %) and (91.5–92.4 %). Strain X14M-14 contained MK-6 as the major respiratory quinone, and phosphatidylethanolamine and two unknown lipids as the major polar lipids. The major cellular fatty acids were iso-C, iso-C G and iso-C 3-OH. The presence of iso-C G as a predominant fatty acid could distinguish this strain clearly from the most closely related genera in the family . The DNA G+C content was 36.6 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain X14M-14 represents a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is X14M-14 ( = CGMCC 1.12924 = KCTC 42675).

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2016-05-01
2019-12-08
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990;). Basic local alignment search tool. J Mol Biol 215: 403–410 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bernardet J. F. , Segers P. , Vancanneyt M. , Berthe F. , Kersters K. , Vandamme P. . ( 1996;). Cutting a gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46: 128–148 [CrossRef].
    [Google Scholar]
  3. Bernardet J. F. , Nakagawa Y. , Holmes B. . Subcommittee on the taxonomy of Flavobacterium Cytophaga-like bacteria of the International Committeeon Systematics of Prokaryotes . ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [PubMed].
    [Google Scholar]
  4. Biebl H. , Pukall R. , Lünsdorf H. , Schulz S. , Allgaier M. , Tindall B. J. , Wagner-Döbler I. . ( 2007;). Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium hamelinense . Int J Syst Evol Microbiol 57: 1095–1107 [CrossRef] [PubMed].
    [Google Scholar]
  5. Bischel M. D. , Austin J. H. . ( 1963;). A modified benzidine method for the chromatographic detection of sphingolipids and acid polysaccharides. Biochim Biophys Acta 70: 598–600 [CrossRef] [PubMed].
    [Google Scholar]
  6. Bowman J. P. , Nichols D. S. . ( 2002;). Aequorivita gen. nov., a member of the family Flavobacteriaceae isolated from terrestrial and marine Antarctic habitats. Int J Syst Evol Microbiol 52: 1533–1541 [PubMed].
    [Google Scholar]
  7. Chen Y. G. , Cui X. L. , Zhang Y. Q. , Li W. J. , Wang Y. X. , Kim C. J. , Lim J. M. , Xu L. H. , Jiang C. L. . ( 2008;). Salinimicrobium terrae sp. nov., isolated from saline soil, and emended description of the genus Salinimicrobium . Int J Syst Evol Microbiol 58: 2501–2504 [CrossRef] [PubMed].
    [Google Scholar]
  8. Dong X. Z. , Cai M. Y. . ( 2001;). Determinative Manual for Routine Bacteriology Beijing: Scientific Press; (English translation).
    [Google Scholar]
  9. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  10. Fitch W. M. . ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  11. Garrity G. M. , Holt J. G. . ( 2001;). The road map to the Manual. [CrossRef] In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 1, pp. 119–166. Edited by Boone D. R. , Castenholz R. W. , Garrity G. M. . New York: Springer;.
    [Google Scholar]
  12. Gunstone F. D. , Jacobsberg F. R. . ( 1972;). Fatty acids, part 35: the preparation and properties of a complete series of methyl epoxyoctadecanoates. Chem Phys Lipids 9: 26–34 [CrossRef].
    [Google Scholar]
  13. Jooste , Kates M. . ( 1986;). Techniques of Lipidology , 2nd edn. Amsterdam: Elsevier;.
    [Google Scholar]
  14. Kim J. H. , Kim K. Y. , Hahm Y. T. , Kim B. S. , Chun J. , Cha C. J. . ( 2008;). Actibacter sediminis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. Int J Syst Evol Microbiol 58: 139–143 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  16. Kimura M. . ( 1983;). The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  17. Komagata K. , Suzuki K. . ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19: 161–207 [CrossRef].
    [Google Scholar]
  18. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. , other authors . ( 2007;). Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947–2948 [CrossRef] [PubMed].
    [Google Scholar]
  19. Lee S. Y. , Park S. , Oh T. K. , Yoon J. H. . ( 2012;). Salinimicrobium gaetbulicola sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 62: 1027–1031 [CrossRef] [PubMed].
    [Google Scholar]
  20. Lim J. M. , Jeon C. O. , Lee S. S. , Park D. J. , Xu L. H. , Jiang C. L. , Kim C. J. . ( 2008;). Reclassification of Salegentibacter catena Ying et al. 2007 as Salinimicrobium catena gen. nov., comb. nov. and description of Salinimicrobium xinjiangense sp. nov., a halophilic bacterium isolated from Xinjiang province in China. Int J Syst Evol Microbiol 58: 438–442 [CrossRef] [PubMed].
    [Google Scholar]
  21. Liu J. J. , Zhang X. Q. , Pan J. , Sun C. , Zhang Y. , Li C. Q. , Zhu X. F. , Wu M. . ( 2013;). Aequorivita viscosa sp. nov., isolated from an intertidal zone, and emended descriptions of Aequorivita antarctica and Aequorivita capsosiphonis . Int J Syst Evol Microbiol 63: 3192–3196 [CrossRef] [PubMed].
    [Google Scholar]
  22. Marmur J. , Doty P. . ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118 [CrossRef] [PubMed].
    [Google Scholar]
  23. McBride M. . ( 2014;). The family Flavobacteriaceae . . In The Prokaryotes, pp. 643–676. Edited by Rosenberg E. , DeLong E. , Lory S. , Stackebrandt E. , Thompson F. . Berlin: Springer;.
    [Google Scholar]
  24. Nedashkovskaya O. I. , Vancanneyt M. , Kim S. B. , Han J. , Zhukova N. V. , Shevchenko L. S. . ( 2010;). Salinimicrobium marinum sp. nov., a halophilic bacterium of the family Flavobacteriaceae, and emended descriptions of the genus Salinimicrobium and Salinimicrobium catena . Int J Syst Evol Microbiol 60: 2303–2306 [CrossRef] [PubMed].
    [Google Scholar]
  25. Nokhal T. H. , Schlegel H. G. . ( 1983;). Taxonomic study of Paracoccus denitrificans . Int J Syst Bacteriol 33: 26–37 [CrossRef].
    [Google Scholar]
  26. Park S. C. , Baik K. S. , Kim M. S. , Kim S. S. , Kim S. R. , Oh M. J. , Kim D. , Bang B. H. , Seong C. N. . ( 2009;). Aequorivita capsosiphonis sp. nov., isolated from the green alga Capsosiphon fulvescens, and emended description of the genus Aequorivita . Int J Syst Evol Microbiol 59: 724–728 [CrossRef] [PubMed].
    [Google Scholar]
  27. Park S. C. , Hwang Y. M. , Lee J. H. , Baik K. S. , Seong C. N. . ( 2013;). Algibacter agarivorans sp. nov. and Algibacter agarilyticus sp. nov., isolated from seawater, reclassification of Marinivirga aestuarii as Algibacter aestuarii comb. nov. and emended description of the genus Algibacter . Int J Syst Evol Microbiol 63: 3494–3500 [CrossRef] [PubMed].
    [Google Scholar]
  28. Reichenbach H. . ( 1992;). The order Cytophagales . . [CrossRef] In The Prokaryotes, pp. 3631–3675. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K. H. . New York: Springer;.
    [Google Scholar]
  29. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  30. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids Technical Note 101 Newark, DE: MIDI;.
    [Google Scholar]
  31. Shakeela Q. , Shehzad A. , Tang K. , Zhang Y. , Zhang X. H. . ( 2015;). Ichthyenterobacterium magnum gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from olive flounder (Paralichthys olivaceus). Int J Syst Evol Microbiol 65: 1186–1192 [CrossRef] [PubMed].
    [Google Scholar]
  32. Subhash Y. , Sasikala Ch. , Ramana Ch. V. . ( 2014;). Salinimicrobium sediminis sp. nov., isolated from a deep-sea sediment. Int J Syst Evol Microbiol 64: 984–988 [CrossRef] [PubMed].
    [Google Scholar]
  33. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  34. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173: 697–703 [PubMed].
    [Google Scholar]
  35. Ying J. Y. , Liu Z. P. , Wang B. J. , Dai X. , Yang S. S. , Liu S. J. . ( 2007;). Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter . Int J Syst Evol Microbiol 57: 219–222 [CrossRef] [PubMed].
    [Google Scholar]
  36. Zhang X. Y. , Liu A. , Liu C. , Li H. , Li G. W. , Xu Z. , Chen X. L. , Zhou B. C. , Zhang Y. Z. . ( 2013;). Arenitalea lutea gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from intertidal sand. Int J Syst Evol Microbiol 63: 2853–2858 [CrossRef] [PubMed].
    [Google Scholar]
  37. Zhang R. G. , Tan X. , Liang Y. , Meng T. Y. , Liang H. Z. , Lv J. . ( 2014;). Description of Chishuiella changwenlii gen. nov., sp. nov., isolated from freshwater, and transfer of Wautersiella falsenii to the genus Empedobacter as Empedobacter falsenii comb. nov. Int J Syst Evol Microbiol 64: 2723–2728 [CrossRef] [PubMed].
    [Google Scholar]
  38. Zhong Z. P. , Liu Y. , Liu H. C. , Wang F. , Zhou Y. G. , Liu Z. P. . ( 2014;). Roseibium aquae sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 64: 2812–2818 [CrossRef] [PubMed].
    [Google Scholar]
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