1887

Abstract

A novel Gram-stain-negative, straight rod-shaped, non-pigmented, slightly halophilic and alkaliphilic bacterium, designated strain GBPy7, was isolated from a sample of the coastal-marine wetland Gomishan in Iran. Cells of strain GBPy7 were motile. Growth occurred on media with 1–15 % (w/v) NaCl (optimum 3 %), at pH 7–10 (optimum pH 8.5) and at 4–45 °C (optimum 37 °C). Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that strain GBPy7 belonged to the family . Its closest relatives were AIS (98.1 % 16S rRNA gene sequence similarity) and other species (95.9–94.2 %), together with CL-SP19 (94.3 %) and F23 (94.3 %). The major cellular fatty acids of the isolate were iso-C, iso-C, iso-Cω9 and Cω7 and its polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown aminophospholipid. Cells of strain GBPy7 contained ubiquinone Q-8. The G+C content of the genomic DNA of this strain was 51.6 mol%. The level of DNA–DNA relatedness between strain GBPy7 and IBRC-M 10414 was 21 %. The physiological, biochemical, genotypic and phylogenetic differences between strain GBPy7 and other previously described taxa indicate that the strain represents a novel species of the genus within the family , for which the name sp. nov. is proposed. The type strain is GBPy7 ( = IBRC-M 10763 = CECT 8339).

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2016-05-01
2019-10-15
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