1887

Abstract

A Gram-stain-negative and non-motile bacterial strain designated 9O-5 was isolated from an abandoned lead–zinc mine in Meizhou, Guangdong Province, southern China. The isolate was orange-pigmented, aerobic, and oxidase- and catalase-positive. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 9O-5 belongs to the genus and was closely related to DSM 15867 (97.6 % similarity), FA2 (96.9 %) and 9NM-8 (96.8 %). Mean DNA–DNA relatedness between strain 9O-5 and DSM 15867 was only 47.1 ± 4.9 %. The major fatty acids were Cω7, C 2-OH and summed feature 3 (Cω7 and/or Cω6). It contained Q-10 as the predominant respiratory quinone and -homospermidine as the major polyamine. The polar lipids were sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, five unidentified phospholipids and six unidentified lipids. The genomic DNA G+C content of strain 9O-5 was 69.1 ± 0.1 mol%. Based on the data from this polyphasic taxonomic study, strain 9O-5 should be considered as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 9O-5 ( = CGMCC 1.15330 = KCTC 42759).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000989
2016-05-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/5/2046.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000989&mimeType=html&fmt=ahah

References

  1. Akbar A., Chen C., Zhu L., Xin K., Cheng J., Yang Q., Zhao L., Zhang L., Shen X. 2015; Sphingomonas hylomeconis sp. nov., isolated from the stem of Hylomecon japonica . Int J Syst Evol Microbiol 65:4025–4031 [View Article][PubMed]
    [Google Scholar]
  2. Busse H. J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [View Article]
    [Google Scholar]
  3. Busse H. J., Denner E.B.M., Buczolits S., Salkinoja-Salonen M., Bennasar A., Kämpfer P. 2003; Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas . Int J Syst Evol Microbiol 53:1253–1260 [View Article][PubMed]
    [Google Scholar]
  4. Busse H. J., Hauser E., Kämpfer P. 2005; Description of two novel species, Sphingomonas abaci sp. nov. and Sphingomonas panni sp. nov. Int J Syst Evol Microbiol 55:2565–2569 [View Article][PubMed]
    [Google Scholar]
  5. Chen H., Jogler M., Rohde M., Klenk H. P., Busse H. J., Tindall B. J., Spröer C., Overmann J. 2012; Reclassification and emended description of Caulobacter leidyi as Sphingomonas leidyi comb. nov., and emendation of the genus Sphingomonas . Int J Syst Evol Microbiol 62:2835–2843 [View Article][PubMed]
    [Google Scholar]
  6. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  8. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  9. Feng G. D., Yang S. Z., Wang Y. H., Zhao G. Z., Deng M. R., Zhu H. H. 2014a; Sphingomonas gimensis sp. nov., a novel Gram-negative bacterium isolated from abandoned lead-zinc ore mine. Antonie van Leeuwenhoek 105:1091–1097 [View Article][PubMed]
    [Google Scholar]
  10. Feng G. D., Yang S. Z., Wang Y. H., Zhang X. X., Zhao G. Z., Deng M. R., Zhu H. H. 2014b; Description of a Gram-negative bacterium, Sphingomonas guangdongensis sp. nov. Int J Syst Evol Microbiol 64:1697–1702 [View Article][PubMed]
    [Google Scholar]
  11. Feng G. D., Wang Y. H., Li Y. X., Zhu H. H. 2015; Deinococcus metalli sp. nov., isolated from abandoned lead-zinc mine. Int J Syst Evol Microbiol 65:3457–3461 [View Article][PubMed]
    [Google Scholar]
  12. Han S. I., Lee J. C., Ohta H., Whang K. S. 2014; Sphingomonas oligoaromativorans sp. nov., an oligotrophic bacterium isolated from a forest soil. Int J Syst Evol Microbiol 64:1679–1684 [View Article][PubMed]
    [Google Scholar]
  13. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [View Article]
    [Google Scholar]
  14. Kämpfer P., Busse H. J., McInroy J. A., Glaeser S. P. 2015; Sphingomonas zeae sp. nov., isolated from the stem of Zea mays . Int J Syst Evol Microbiol 65:2542–2548 [View Article][PubMed]
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  16. Kim S. J., Moon J. Y., Lim J. M., Ahn J. H., Weon H. Y., Ahn T. Y., Kwon S. W. 2014; Sphingomonas aerophila sp. nov. and Sphingomonas naasensis sp. nov., isolated from air and soil, respectively. Int J Syst Evol Microbiol 64:926–932 [View Article][PubMed]
    [Google Scholar]
  17. Kim J. H., Kim S. H., Kim K. H., Lee P. C. 2015; Sphingomonas lacus sp. nov., an astaxanthin-dideoxyglycoside-producing species isolated from soil near a pond. Int J Syst Evol Microbiol 65:2824–2830 [View Article][PubMed]
    [Google Scholar]
  18. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  19. Lee K. C., Kim K. K., Kim J. S., Kim D. S., Ko S. H., Yang S. H., Kim S. G., Lee J. S. 2015; Sphingomonas vulcanisoli sp. nov., isolated from soil of a lava forest. Int J Syst Evol Microbiol 65:3320–3325 [View Article][PubMed]
    [Google Scholar]
  20. Liu Q., Liu H. C., Zhang J. L., Zhou Y. G., Xin Y. H. 2015a; Sphingomonas psychrolutea sp. nov., a psychrotolerant bacterium isolated from glacier ice. Int J Syst Evol Microbiol 65:2955–2959 [View Article][PubMed]
    [Google Scholar]
  21. Liu Y., Yao S., Lee Y. J., Cao Y., Zhai L., Zhang X., Su J., Ge Y., Kim S. G., Cheng C. 2015b; Sphingomonas morindae sp. nov., isolated from Noni (Morinda citrifolia L.) branch. Int J Syst Evol Microbiol 65:2817–2823 [View Article][PubMed]
    [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  24. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  25. Scherer P., Kneifel H. 1983; Distribution of polyamines in methanogenic bacteria. J Bacteriol 154:1315–1322[PubMed]
    [Google Scholar]
  26. Sheu S. Y., Chen Y. L., Chen W. M. 2015; Sphingomonas fonticola sp. nov., isolated from spring water. Int J Syst Evol Microbiol 65:4495–4502 [View Article][PubMed]
    [Google Scholar]
  27. Takeuchi M., Hamana K., Hiraishi A. 2001; Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417 [View Article][PubMed]
    [Google Scholar]
  28. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  29. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology pp 330–393Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P.A.D., Kandler O., Krichevsky M. I., Moore L. H., Moore W.E.C., Murray R.G.E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  31. Wei S., Wang T., Liu H., Zhang C., Guo J., Wang Q., Liang K., Zhang Z. 2015; Sphingomonas hengshuiensis sp. nov., isolated from lake wetland. Int J Syst Evol Microbiol 65:4644–4649 [View Article]
    [Google Scholar]
  32. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703[PubMed]
    [Google Scholar]
  33. Yabuuchi E., Yano I., Oyaizu H., Hashimoto Y., Ezaki T., Yamamoto H. 1990; Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas . Microbiol Immunol 34:99–119 [View Article][PubMed]
    [Google Scholar]
  34. Yabuuchi E., Kosako Y., Fujiwara N., Naka T., Matsunaga I., Ogura H., Kobayashi K. 2002; Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola . Int J Syst Evol Microbiol 52:1485–1496[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000989
Loading
/content/journal/ijsem/10.1099/ijsem.0.000989
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error