1887

Abstract

A novel actinomycete, strain KC-112, was isolated from soil collected from Similan Islands, Phang-Nga Province, Thailand. The strain exhibited morphological and chemotaxonomic characteristics consistent with those of members of the genus . The formation of smooth spiral spore chains was observed on aerial mycelia. -Diaminopimelic acid was detected in whole-cell hydrolysates, but no diagnostic sugars were detected and the strain lacked mycolic acids. The -acyl type of muramic acid was acetyl. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The predominant cellular fatty acids were anteiso-C, anteiso-C, iso-C and C. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unknown phospholipid, an unknown aminolipid, unknown lipids and an unknown glycolipid. The DNA G+C content was 73 mol%. On the basis of 16S rRNA gene sequence analysis, strain KC-112 was closely related to NBRC 13042 (98.8 % 16S rRNA gene sequence similarity), NBRC 13438 (98.8 %) and NBRC 14222 (98.8 %). DNA–DNA relatedness values among strain KC-112 and type strains of closely related species were lower than 70 %. On the basis of evidence from this taxonomic study using a polyphasic approach, strain KC-112 represents a novel species of the genus , namely sp. nov. The type strain is KC-112 ( = KCTC 29502 = NBRC 110085 = PCU 347 = TISTR 2401).

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2016-05-01
2019-10-15
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References

  1. Acar J. F. , Goldstein F. W. . ( 1991;). Disk susceptibility test. . In Antibiotics in Laboratory Medicine , 3rd edn., pp. 17–52. Edited by Lorian V. . Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  2. Arai T. . ( 1975;). Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan;.
    [Google Scholar]
  3. Becker B. , Lechevalier M. P. , Lechevalier H. A. . ( 1965;). Chemical composition of cell-wall preparation from strains of various from-genera of aerobic actinomycetes. Appl Microbiol 13: 236–243 [PubMed].
    [Google Scholar]
  4. Bérdy J. . ( 2005;). Bioactive microbial metabolites. J Antibiot (Tokyo) 58: 1–26 [CrossRef] [PubMed].
    [Google Scholar]
  5. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100: 221–230 [CrossRef] [PubMed].
    [Google Scholar]
  6. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39: 224–229 [CrossRef].
    [Google Scholar]
  7. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  8. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  9. Kämpfer P. . ( 2012;). Genus I Streptomyces Waksman and Henrici 1943, 339AL emend. Witt and Stackebrandt 1990, 370 emend. Witt and Stackebrandt 1990, 370 emend. Wellington, Stackebrandt, Sanders, Wolstrup and Jorgensen 1992, 159. . In Bergey's Manual of Systematic Bacteriology vol. 5 , 2nd edn., pp. 1455–1767. Edited by Goodfellow M. , Kämpfer P. , Busse H.-J. , Trujillo M. E. , Suzuki K. , Ludwig W. , Whitman W. B. . New York: Springer;.[CrossRef]
    [Google Scholar]
  10. Kelly K. L. . ( 1964;). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;.
    [Google Scholar]
  11. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kimura M. . ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kluge A. G. , Farris J. S. . ( 1969;). Quantitative phyletics and the evolution of anurans. Syst Zool 18: 1–32 [CrossRef].
    [Google Scholar]
  14. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  15. Parte A. C. . ( 2015;). List of prokaryotic names with standing in nomenclature: a folder available on the Internet. [Last full update 24 November 2015]. http://www.bacterio.net .
  16. Saito H. , Miura K. I. . ( 1963;). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72: 619–629 [CrossRef] [PubMed].
    [Google Scholar]
  17. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  18. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  19. Shirling E. B. , Gottlieb D. . ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16: 313–340 [CrossRef].
    [Google Scholar]
  20. Sripreechasak P. , Suwanborirux K. , Tanasupawat S. . ( 2014;). Characterization and antimicrobial activity of Streptomyces strains from soils in southern Thailand. J App Pharm Sci 4: 24–31 [CrossRef].
    [Google Scholar]
  21. Takahashi Y. , Matsumoto A. , Seino A. , Ueno J. , Iwai Y. , Omura S. . ( 2002;). Streptomyces avermectinius sp. nov., an avermectin-producing strain. Int J Syst Evol Microbiol 52: 2163–2168 [PubMed].
    [Google Scholar]
  22. Tamaoka J. , Komagata K. . ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25: 125–128 [CrossRef].
    [Google Scholar]
  23. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  24. Tomiyasu I. . ( 1982;). Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . J Bacteriol 151: 828–837 [PubMed].
    [Google Scholar]
  25. Uchida K. , Aida K. . ( 1984;). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 37: 131–134 [CrossRef].
    [Google Scholar]
  26. Waksman S. A. , Henrici A. T. . ( 1943;). The nomenclature and classification of the actinomycetes. J Bacteriol 46: 337–341 [PubMed].
    [Google Scholar]
  27. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P.A.D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W.E.C. , Murray R.G.E. , other authors . ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
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