1887

Abstract

A novel actinomycete, strain KC-112, was isolated from soil collected from Similan Islands, Phang-Nga Province, Thailand. The strain exhibited morphological and chemotaxonomic characteristics consistent with those of members of the genus . The formation of smooth spiral spore chains was observed on aerial mycelia. -Diaminopimelic acid was detected in whole-cell hydrolysates, but no diagnostic sugars were detected and the strain lacked mycolic acids. The -acyl type of muramic acid was acetyl. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The predominant cellular fatty acids were anteiso-C, anteiso-C, iso-C and C. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unknown phospholipid, an unknown aminolipid, unknown lipids and an unknown glycolipid. The DNA G+C content was 73 mol%. On the basis of 16S rRNA gene sequence analysis, strain KC-112 was closely related to NBRC 13042 (98.8 % 16S rRNA gene sequence similarity), NBRC 13438 (98.8 %) and NBRC 14222 (98.8 %). DNA–DNA relatedness values among strain KC-112 and type strains of closely related species were lower than 70 %. On the basis of evidence from this taxonomic study using a polyphasic approach, strain KC-112 represents a novel species of the genus , namely sp. nov. The type strain is KC-112 ( = KCTC 29502 = NBRC 110085 = PCU 347 = TISTR 2401).

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2016-05-01
2024-12-07
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