1887

Abstract

A novel actinomycete, strain KC-112, was isolated from soil collected from Similan Islands, Phang-Nga Province, Thailand. The strain exhibited morphological and chemotaxonomic characteristics consistent with those of members of the genus . The formation of smooth spiral spore chains was observed on aerial mycelia. -Diaminopimelic acid was detected in whole-cell hydrolysates, but no diagnostic sugars were detected and the strain lacked mycolic acids. The -acyl type of muramic acid was acetyl. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The predominant cellular fatty acids were anteiso-C, anteiso-C, iso-C and C. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unknown phospholipid, an unknown aminolipid, unknown lipids and an unknown glycolipid. The DNA G+C content was 73 mol%. On the basis of 16S rRNA gene sequence analysis, strain KC-112 was closely related to NBRC 13042 (98.8 % 16S rRNA gene sequence similarity), NBRC 13438 (98.8 %) and NBRC 14222 (98.8 %). DNA–DNA relatedness values among strain KC-112 and type strains of closely related species were lower than 70 %. On the basis of evidence from this taxonomic study using a polyphasic approach, strain KC-112 represents a novel species of the genus , namely sp. nov. The type strain is KC-112 ( = KCTC 29502 = NBRC 110085 = PCU 347 = TISTR 2401).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000987
2016-05-01
2020-04-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/5/2030.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000987&mimeType=html&fmt=ahah

References

  1. Acar J. F., Goldstein F. W.. 1991; Disk susceptibility test. In Antibiotics in Laboratory Medicine, 3rd edn. pp17–52Edited by Lorian V.. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  2. Arai T.. 1975; Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan;
    [Google Scholar]
  3. Becker B., Lechevalier M. P., Lechevalier H. A.. 1965; Chemical composition of cell-wall preparation from strains of various from-genera of aerobic actinomycetes. Appl Microbiol13:236–243[PubMed]
    [Google Scholar]
  4. Bérdy J.. 2005; Bioactive microbial metabolites. J Antibiot (Tokyo)58:1–26 [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39:224–229 [CrossRef]
    [Google Scholar]
  7. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  9. Kämpfer P.. 2012; Genus I Streptomyces Waksman and Henrici 1943, 339AL emend. Witt and Stackebrandt 1990, 370 emend. Witt and Stackebrandt 1990, 370 emend. Wellington, Stackebrandt, Sanders, Wolstrup and Jorgensen 1992, 159. In Bergey's Manual of Systematic Bacteriologyvol. 5, 2nd edn. pp1455–1767Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K., Ludwig W., Whitman W. B.. New York: Springer;[CrossRef]
    [Google Scholar]
  10. Kelly K. L.. 1964; Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  12. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  13. Kluge A. G., Farris J. S.. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool18:1–32 [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  15. Parte A. C.. 2015; List of prokaryotic names with standing in nomenclature: a folder available on the Internet. [Last full update 24 November 2015]. http://www.bacterio.net
  16. Saito H., Miura K. I.. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta72:619–629 [CrossRef][PubMed]
    [Google Scholar]
  17. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  18. Sasser M.. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  19. Shirling E. B., Gottlieb D.. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol16:313–340 [CrossRef]
    [Google Scholar]
  20. Sripreechasak P., Suwanborirux K., Tanasupawat S.. 2014; Characterization and antimicrobial activity of Streptomyces strains from soils in southern Thailand. J App Pharm Sci4:24–31 [CrossRef]
    [Google Scholar]
  21. Takahashi Y., Matsumoto A., Seino A., Ueno J., Iwai Y., Omura S.. 2002; Streptomyces avermectinius sp. nov., an avermectin-producing strain. Int J Syst Evol Microbiol52:2163–2168[PubMed]
    [Google Scholar]
  22. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  23. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  24. Tomiyasu I.. 1982; Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . J Bacteriol151:828–837[PubMed]
    [Google Scholar]
  25. Uchida K., Aida K.. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol37:131–134 [CrossRef]
    [Google Scholar]
  26. Waksman S. A., Henrici A. T.. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol46:337–341[PubMed]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P.A.D., Kandler O., Krichevsky M. I., Moore L. H., Moore W.E.C., Murray R.G.E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000987
Loading
/content/journal/ijsem/10.1099/ijsem.0.000987
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error