1887

Abstract

A novel bacterium, strain 7515T-26, was isolated from an air sample collected in Taean region, Republic of Korea. Cells were aerobic, Gram-stain-positive, non-flagellated cocci, growing in the temperature, pH and NaCl ranges of 10–33 °C, pH 5.0–9.0 and 0–2 % (w/v). It shared high 16S rRNA gene sequence similarity with EL-17A (97.6 %), FA2 (96.9 %) and FA1 (96.9 %), showing high sequence similarities of 96.5–97.6 % with members of the genus . Phylogenetic trees based on 16S rRNA gene sequences showed that strain 7515T-26 and members of the genus formed a compact cluster separable from other genera. The isolate contained anteiso-C, iso-C 3-OH and iso-C as the major cellular fatty acids, and MK-9(H) as the predominant isoprenoid quinone. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unknown phospholipids and one unknown lipid, and the DNA G+C content was 73.1 mol%. The peptidoglycan type was A3γ. It showed DNA–DNA hybridization values of less than 70 % with EL-17A. On the basis of the evidence from this polyphasic study, a novel species, sp. nov., is proposed. The type strain is 7515T-26 ( = KACC 17306 = DSM 27139).

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2016-05-01
2019-10-22
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References

  1. Breznak J. A., Costilow R. N.. ( 1994;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  2. Collins M. D., Goodfellow M., Minnikin D. E.. ( 1980;). Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol 118: 29–37 [PubMed].
    [Google Scholar]
  3. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  4. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  5. Gonzalez J. M., Saiz-Jimenez C.. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4: 770–773 [CrossRef] [PubMed].
    [Google Scholar]
  6. Hamada M., Tamura T., Yamamura H., Suzuki K., Hayakawa M.. ( 2012;). Lysinimicrobium mangrovi gen. nov., sp. nov., an actinobacterium isolated from the rhizosphere of a mangrove. Int J Syst Evol Microbiol 62: 1731–1735 [CrossRef] [PubMed].
    [Google Scholar]
  7. Iwai K., Aisaka K., Suzuki M.. ( 2010;). Friedmanniella luteola sp. nov., Friedmanniella lucida sp. nov., Friedmanniella okinawensis sp. nov. and Friedmaniella sagamiharensis sp. nov., isolated from spiders. Int J Syst Evol Microbiol 60: 113–120 [CrossRef] [PubMed].
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  9. Lawson P. A., Collins M. D., Schumann P., Tindal B. J., Hirsch P., Labrenz M.. ( 2000;). New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp. nov. and Friedmanniella [correction of Friedmannielly] lacustris sp. nov. Syst Appl Microbiol 23: 219–229 [CrossRef] [PubMed].
    [Google Scholar]
  10. Maszenan A. M., Seviour R. J., Patel B.K.C., Schumann P., Burghardt J., Webb R. I., Soddell J. A., Rees G. N.. ( 1999;). Friedmanniella spumicola sp. nov. and Friedmanniella capsulata sp. nov. from activated sludge foam: gram-positive cocci that grow in aggregates of repeating groups of cocci. Int J Syst Bacteriol 49: 1667–1680 [CrossRef] [PubMed].
    [Google Scholar]
  11. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  12. Pruesse E., Peplies J., Glöckner F. O.. ( 2012;). SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28: 1823–1829 [CrossRef] [PubMed].
    [Google Scholar]
  13. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  14. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  15. Schleifer K. H., Seidl P. H.. ( 1985;). Chemical composition and structure of murein. . In Chemical Methods in Bacterial Systematics, pp. 201–219. Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;.
    [Google Scholar]
  16. Schumann P., Prauser H., Rainey F. A., Stackebrandt E., Hirsch P.. ( 1997;). Friedmanniella antarctica gen. nov., sp. nov., an ll-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 47: 278–283 [CrossRef] [PubMed].
    [Google Scholar]
  17. Seldin L., Dubnau D.. ( 1985;). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35: 151–154 [CrossRef].
    [Google Scholar]
  18. Tamura K., Nei M.. ( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10: 512–526 [PubMed].
    [Google Scholar]
  19. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  20. Waksman S. A.. ( 1961;). The Actinomycetes. Classification, Identification, Descriptions of Genera and Speciesvol. 2 Baltimore: Williams & Wilkins;.
    [Google Scholar]
  21. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173: 697–703 [PubMed].
    [Google Scholar]
  22. Zhang X., Zhang J., Zhang Y., Xin Y., He H.. ( 2013;). Friedmanniella flava sp. nov., a soil actinomycete. Int J Syst Evol Microbiol 63: 1771–1775 [CrossRef] [PubMed].
    [Google Scholar]
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