1887

Abstract

A halotolerant actinobacterial strain, designated EGI 80423, was isolated from a desert soil of Xinjiang, north-west China, and subjected to a polyphasic taxonomic characterization. Strain EGI 80423 grew at pH 7.0–10.0 and with 0–14.0 % (w/v) NaCl, optimally at pH 8.0–9.0 and with 2.0–4.0 % (w/v) NaCl. Cells of strain EGI 80423 were Gram-stain-positive, non-motile cocci with diameters of 0.6–0.8 μm. The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was Orn ← Glu. The major fatty acids identified were iso-Cω9, iso-C and iso-C. The predominant menaquinone was MK-8(H), while the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unknown phospholipids, two unknown glycolipids, six unknown phosphoglycolipids and five unknown polar lipids. The G+C content of the genomic DNA was 72.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EGI 80423 clustered with the single member of the genus . Sequence similarity between strain EGI 80423 and NBRC 16434. Because the type strain has been provided by NBRC, Japan was 97.7 %. The DNA–DNA relatedness value between strain EGI 80423 and NBRC 16434 was 36.84 %. Based on morphological, chemotaxonomic and phylogenetic characteristics, and DNA–DNA hybridization data, strain EGI 80423 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 80423 ( = CGMCC 1.14989 = KCTC 39700). The description of the genus has also been emended.

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2016-04-01
2022-01-23
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